Isolation of Staphylococcus schleiferi from healthy dogs and dogs with otitis, pyoderma, or both

May, Elizabeth; Hnilica, Keith A.; Frank, Linda A.; Jones, Rebekah D.; Bemis, David A.

doi:10.2460/javma.2005.227.928

Cited by 66 publications

(62 citation statements)

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“…foi isolado no conduto auditivo de 15/29 (51,7%) dos animais analisados, sendo encontrado em todas as espécies pesquisadas com exceção de E. barbara. Esta bactéria é frequentemente isolada de diferentes espécies de animais selvagens e domésticos e é uma das bactérias mais importantes para a medicina veterinária devido a sua associação com várias enfermidades e sua alta resistência a antibióticos (Mangini et al 2002, Vintov et al 2003, May et al 2005, Verona & Pissinatti 2006.…”

Section: Discussionunclassified

Microbiologia de swabs retais e otológicos em carnívoros silvestres do zoológico do Parque Estadual de Dois Irmãos, Pernambuco

et al. 2012

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Pesq. Vet. Bras. 32(2):159-164, fevereiro 2012 159 RESUMO.-O estudo epidemiológico de patógenos em populações selvagens no ambiente in situ e ex situ é fundamental para implementação de programas de prevenção, controle e monitoramento de enfermidades e para elabo- The epidemiological study of pathogens in wild populations in the environment in situ and ex situ is essential for implementation of prevention, control and monitoring of diseases and for development of public policies for public and animal health. The study was conducted at the Zoo of Parque Estadual de Dois Irmãos, Recife/PE, Brazil, from January to July 2011, where we collected anal and otological swabs from 29 Zoo's captive wild carnivores. The analysis of the otological swabs showed that 1/29 (3.4%) were positive for Malassezia pachydermatis on direct examination. In the microbiological examination of otologic swabs we found mainly bacteriae of the genus Bacillus 16/29 (55.2%), followed by Staphylococcus 15/29 (51.7%), Escherichia coli 7/29 (24.1%), Streptobacillus 1/29 (3.4%), Micrococcus 1/29 (3.4%) and Klebsiella 1/29 (3.4%). Regarding the presumptive test for the genus Salmonella from rectal swabs, positive samples were observed in six foxes (Cerdocyon thous) and a raccoon (Procyon cancrivorus). The isolation of Salmonella spp. in C. thous and P. cancrivorus indicates risks to public health, especially for zoo professionals who work directly with these animals. This research reinforces the importance of establishing surveillance strategies aimed at prevention, control and monitoring of potential reservoirs of the etiologic agents of infectious and parasitic diseases in the environment of zoos. Microbiologia de INTRODUÇÃOO estudo epidemiológico de patógenos em populações selvagens no ambiente in situ e ex situ é fundamental para implementação de programas de prevenção, controle e monitoramento de enfermidades e para elaboração de políticas públicas de saúde pública e animal (Jorge et al. 2010). Salmonella spp. são bactérias Gram-negativas, móveis, que podem infectar a maioria das espécies de animais, entre mamíferos, aves e répteis, inclusive os humanos, sendo considerada uma zoonose de grande importância fecal--oral, ocorrendo principalmente pelo conta to direto ou indireto com animais infectados ou pela ingestão de alimentos ou água contaminada (Carvalho 2006).A otite é uma enfermidade multifatorial resultante do processo inϐlamatório da cavidade auditiva tendo origem na região externa e podendo prolongar-se até o sistema vestíbulo-coclear. Esse processo migratório geralmente é acompanhado por complicações mais graves e extensas (Greene 1993). Malassezia pachydermatis é uma levedura de natureza oportunista, tornando-se patogênica quando há uma alteração no ambiente microbiológico da superϐície da pele ou no sistema imunológico do hospedeiro levando a um quadro de otite (Mendes et al. 2011). Outras espécies que também podem provocar otite são Malassezia globosa e M. sympodialis (Coutinho et al. 2006).Objetivou-se com este trabalho realizar um...

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Section: Discussionunclassified

Microbiologia de swabs retais e otológicos em carnívoros silvestres do zoológico do Parque Estadual de Dois Irmãos, Pernambuco

et al. 2012

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“…are being increasingly recovered from canine clinical specimens (2,5,14,16,17,21,23). Interpretation of oxacillin resistance in coagulase-positive Staphylococcus pathogens of the Staphylococcus intermedius-S. schleiferi-S. delphini-S. hyicus clade (13) has been an uncertainty for veterinary diagnosticians, because guidelines are predicated on data derived from human clinical studies with S. aureus and coagulase-negative Staphylococcus spp.…”

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“…Procedures to isolate and identify S. aureus, S. intermedius, and S. schleiferi were as previously described (1,5,17). A PBP2a latex agglutination test kit (penicillin-binding protein [PBP2Ј] latex agglutination test; Oxoid Ltd., Basingstoke, Hampshire, England) was applied, following oxacillin induction, in accordance with the manufacturer's instructions.…”

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Comparison of Tests To Detect Oxacillin Resistance in Staphylococcus intermedius , Staphylococcus schleiferi , and Staphylococcus aureus Isolates from Canine Hosts

Bemis¹,

Jones²,

Hiatt³

et al. 2006

J Clin Microbiol

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“…coagulans wurde 1990 durch Igimi et al aufgrund von DNA-DNA-Hybridisierungsversuchen als Subspecies von S. schleiferi beschrieben. Der Erreger steht in engem Zusammenhang mit Otitiden des Hundes (Igimi et al, 1990;Zdovc et al, 2004;May et al, 2005), wurde inzwischen aber auch beim Menschen in der Mundhöhle (Aparecida de Paiva Martins et al, 2002) bzw. als mutmaßlicher Wundinfektionserreger nachgewiesen (Vandenesch et al, 1994).…”

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Staphylokokken-Enterotoxine: Bildung, Eigenschaften und Nachweis

2007

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Zusammenfassung: Staphylococcus aureus kann sich unter geeigneten Bedingungen in Lebensmitteln vermehren und dabei Enterotoxine bilden, die beim Konsumenten Erbrechen und Durchfall auslösen. Im Gegensatz zu dem Erreger selbst sind diese Toxine sehr widerstandsfähig insbesondere gegenüber hohen Temperaturen und können durch eine küchenmäßige Bearbeitung des sie enthaltenden Lebensmittels im allgemeinen nicht mehr inaktiviert werden. Da demnach die Anwesenheit der Staphylokokken zur Auslösung der Erkrankung nicht mehr erforderlich ist, handelt es sich bei dieser um eine typische Lebensmittelintoxikation. Die Abschätzung einer potentiellen Gesundheitsgefährdung über den Erregernachweis ist daher nur bei rohen Lebensmitteln sinnvoll. Bei Produkten, die im Verlauf ihrer Herstellung einem für die Keime abträglichen Verfahren, z. B. einer Wärmebehandlung, unterzogen wurden, kann der Thermonukleasetest (indirekter Nachweis einer Kontamination mit hoher Anzahl an Staphylokokken, unabhängig davon, ob die Erreger zum Zeitpunkt der Untersuchung noch präsent sind) oder der direkte Enterotoxinnachweis durchgeführt werden. Der Nachweis der Toxingene mit molekularbiologischen Verfahren hat in diesem Zusammenhang nur unterstützende Funktion, da im Fall eines positiven Ergebnisses aus diesem nicht geschlossen werden kann, daß die Gene auch exprimiert wurden, also Toxine im Lebensmittel vorhanden sind. In der vorliegenden Übersichtsarbeit werden der Erreger und seine Enterotoxine sowie die verschiedenen Nachweisverfahren unter besonderer Berücksichtigung der molekularbiologischen Methoden besprochen.Abstract: Under appropriate conditions, Staphylococcus aureus can grow in food and produce enterotoxins which cause vomiting and diarrhoea when ingested. In contrast to the staphylococci these toxins are resistant especially to high temperatures and are not inactivated by usual kitchen practice. As the presence of staphylococci is not essential to cause illness, this condition is a typical food poisoning. The estimation of a potential health risk by targeting the organism is therefore only appropriate to raw food. For products which underwent a procedure detrimental to the bacteria, e. g. heat treatment, testing for thermonuclease (indirect detection of a contamination with high numbers of staphylococci, irrespective of the presence of living bacteria at the time of testing) or the detection of enterotoxins can be applied. In this context, the detection of enterotoxin genes using molecular biological tests provides only supplemental information, as positive results are not evidentiary for gene expression and thus for the presence of toxins in the food. In this review, the causative organism and its toxins as well as methods of detection are discussed with special emphasis on molecular biological methods.

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Isolation of Staphylococcus schleiferi from healthy dogs and dogs with otitis, pyoderma, or both

Abstract: S schleiferi subsp schleiferi was detected in healthy dogs and dogs with otitis and pyoderma. Methicillin-resistant and -susceptible S schleiferi subsp schleiferi and S schleiferi subsp coagulans were detected as the predominant organisms in dogs with otitis.

Cited by 66 publications

References 21 publications

Microbiologia de swabs retais e otológicos em carnívoros silvestres do zoológico do Parque Estadual de Dois Irmãos, Pernambuco

Microbiologia de swabs retais e otológicos em carnívoros silvestres do zoológico do Parque Estadual de Dois Irmãos, Pernambuco

Comparison of Tests To Detect Oxacillin Resistance in Staphylococcus intermedius , Staphylococcus schleiferi , and Staphylococcus aureus Isolates from Canine Hosts

Staphylokokken-Enterotoxine: Bildung, Eigenschaften und Nachweis

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