Isolation of Serologically Active Fucose-Containing Oligosaccharides from Human Blood-Group H Substance

Rege, Vaijayanti P.; Painter, Terence; Watkins, Winifred M.; Morgan, W. T. J.

doi:10.1038/203360a0

Cited by 100 publications

(29 citation statements)

References 19 publications

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“…These enzymes require a precursor oligosaccharide substrate (Fucal -* 2Galf-) known as the H blood group antigen, constructed by a-(1,2)-fucosyltransferase(s) [a(1,2)FTs; GDP-L-fucose:43D-galactoside 2-a-L-fucosyltransferase, EC 2.4.1.69]. Genetic and biochemical observations are consistent with a hypothesis that the H and Secretor (SE) blood group loci (also known as FUTI and FUT2, respectively) correspond to distinct a(1,2)FT genes with tissue-specific expression patterns and close genetic linkage on chromosome 19 (1)(2)(3)(4)(5)(6). Under this two-locus model (2), the human H blood group locus determines expression of the H antigen (as well as A and/or B antigens) in the erythroid lineage, whereas the SE locus controls H expression (and thus A or B antigen expression) in a variety of secretory epithelia and in saliva (Table 1).…”

supporting

confidence: 63%

Molecular basis for H blood group deficiency in Bombay (Oh) and para-Bombay individuals.

Kelly

Эрнст

Larsen

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

169

121

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The penultimate step in the biosynthesis of the human ABO blood group oligosaccharide antigens is catalyzed by a-(1,2)-fucosyltransferase(s) (GDP-L-fucose:flDgalactoside 2-a-L-fucosyltransferase, EC 2.4.1.69), whose expression is determined by the H and Secretor (SE) blood group loci (also known as FUTI and FUT2, respectively). These enzymes construct Fucal -) 2Galh3-linkages, known as H determinants, which are essential precursors to the A and B antigens. Erythrocytes from individuals with the rare Bombay and para-Bombay blood group phenotypes are deficient in H determinants, and thus A and B determinants, as a consequence of apparent homozygosity for null alleles at the H locus.

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supporting

confidence: 63%

Molecular basis for H blood group deficiency in Bombay (Oh) and para-Bombay individuals.

Kelly

Эрнст

Larsen

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

169

121

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“…About 10-60 times as much haptenic substance as isolated antigen (weight basis) was required to give the same extent of inhibition. This agrees well with the proportions of inhibition due to haptens compared to those given by intact ABH(O) antigens [16,24,31]. However, the in vitro activity of ABH(O) glycoproteins is usually 10-100 times as high as that of the human blood group N antigens.…”

Section: Discussionsupporting

confidence: 75%

Anti-N Reagents in Elucidation of the Genetical Basis of Human Blood-Group MN Specificities

Springer¹,

Tegtmeyer²,

Huprikar³

1972

Vox Sanguinis

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Studies of the reactivity of the various anti-human blood group N reagents with isolated human blood group M and N antigens and smaller molecules revealed besides the expected interaction with N antigens that M antigens inhibited all rabbit anti-N sera, Vicia graminea extract and most human anti-N sera. Mild acid hydrolysis of M antigens, which released sialic acid only, led to a large increase or de novo appearance of this inhibitory capacity. The partially hydrolyzed M antigens became temporarily indistinguishable from N antigens, indicating that the product of the N gene is the immediate precursor of the product of the M gene and that the allele to the M gene is an amorph. In the NM biosynthetic pathway, the U'c/a-reactive structure seems to precede the N-specific molecule. Uncovering of N-specific structures paralleled the appearance of terminal β-D-galactopyranosyl structures; their exposure to β-galactosidase resulted in destruction of N specificity. Galactose and some β-D-galactopyranosyl derivatives inhibited the N erythrocyte agglutination by some anti-N sera. Ganglioside I and ‘asialoganglioside’ were inhibitors of anti-N reagents and anti-ganglioside serum reacted preferentially with human blood group N antigens.

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“…%-Configuration of fucose residues in blood-group substances has been determined earlier [15] and especially proved for penta and hexasaccharides described in our previous paper [4]. 8-Galactosidase has completely split terminal galactose residues from oligosaccharides and their fragments and the respective degradation products were formed with a high yield; this has proved unambiguously the P-configuration of galactose residues.…”

Section: The Structure Of Oligosaccharidesmentioning

confidence: 93%

The Structure of Carbohydrate Chains of Blood‐Group Substance

Derevitskaya

Arbatsky

Kochetkov

1978

European Journal of Biochemistry

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Twenty individual higher reduced oligosaccharides, having from seven to eleven monosaccharide units, were isolated after sodium borohydride degradation of blood-group substance H from pig stomach linings. Anion-exchange high-pressure liquid chromatography appears to be a very convenient and effective method for this kind of higher oligosaccharide mixtures separation. The oligosaccharide structures were determined by means of periodate oxidation, methylation analysis, partial acid and enzymic hydrolysis. It has been found that all the oligosaccharides investigated can be divided into four series. The oligosaccharides belonging to each series have the common oligosaccharide fragment to which terminal L-fucose and/or N-acetyl-D-glucosamine residues are attached. Comparison of all the oligosaccharide structures, including tri, penta and hexasaccharides described earlier, shows that .the lower oligosaccharides represent the structural element of the higher oligosaccharides.Recently a high degree of heterogeneity of carbohydrate chains of blood-group substances from different sources has been demonstrated [l -61. The treatment of glycoproteins with alkaline sodium borohydride [7] results in the cleavage of practically undegraded carbohydrate chains as oligosaccharides (see, however [S]). The separation of a complicated oligosaccharide mixture using anion-exchange chromatography in borate buffer 191 gives rise to individual oligosaccharides. The elucidation of their structure makes it possible to draw a more definite conclusion about the carbohydrate chains of bloodgroup substances.Earlier we have reported the structure of trisaccharides [3], penta and hexasaccharides [4] isolated 'from pig blood-group substance H. This paper is concerned with elucidation of the structure of more complex oligosaccharides, having from seven to eleven monosaccharides units, isolated after degradation of the same glycoprotein H.

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Isolation of Serologically Active Fucose-Containing Oligosaccharides from Human Blood-Group H Substance

Cited by 100 publications

References 19 publications

Molecular basis for H blood group deficiency in Bombay (Oh) and para-Bombay individuals.

Molecular basis for H blood group deficiency in Bombay (Oh) and para-Bombay individuals.

Anti-N Reagents in Elucidation of the Genetical Basis of Human Blood-Group MN Specificities

The Structure of Carbohydrate Chains of Blood‐Group Substance

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