Isolation of salmonellae using two forms of Rappaport-Vassiliadis medium and brilliant green agar

McGibbon, Loraine; Quail, Elaine; Fricker, C. R.

doi:10.1016/0168-1605(84)90013-8

Cited by 11 publications

(7 citation statements)

References 20 publications

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“…Cox & Mercuri (1978) compared the use of four media for direct enrichment of poultry samples for salmonellas and showed that selenite-cystine and selenite-brilliant green broths were equally effective. Similar results were reported by Miller & Koburger (1984), who showed that direct enrichment of oysters in selenite cystine broth at elevated temperature (41"43"C) gave more salmonella isolations than did use of the same medium or tetrathionate broth after pre-enrichment in lactose broth.…”

Section: Resuscitation or Pre-enrichment Conditionssupporting

confidence: 86%

“…The media in common use are prepared using a rich basal medium, Lysed blood and various combinations of antimicrobial agents. There have been comparisons of different enrichment broths for the isolation of campylobacters, although both Preston broth Doyle &Romans' (1982) medium have been shown to be satisfactory (Fricker et al 1983b;Fricker 1984~). Microaerobic incubation of enrichment cultures for campylobacters is essential for optimal sensitivity (Fricker 1984c;Fricker et al 1984) and incubation should be continued for at least 48 h (Fricker 1984c;Fricker & Metcalfe 1984) before a negative result is assumed.…”

Section: Enrichment Mediamentioning

confidence: 99%

“…There have been comparisons of different enrichment broths for the isolation of campylobacters, although both Preston broth Doyle &Romans' (1982) medium have been shown to be satisfactory (Fricker et al 1983b;Fricker 1984~). Microaerobic incubation of enrichment cultures for campylobacters is essential for optimal sensitivity (Fricker 1984c;Fricker et al 1984) and incubation should be continued for at least 48 h (Fricker 1984c;Fricker & Metcalfe 1984) before a negative result is assumed. Knowledge of the optimal isolation procedures for campylobacters from sources other than human faeces is extremely limited, however, and there is a need for more research to develop sensitive and universally accepted enrichment procedures, particularly for samples in which sublethally damaged campylobacters are likely to be present.…”

Section: Enrichment Mediamentioning

confidence: 99%

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The isolation of salmonellas and campylobacters

Fricker

1987

Journal of Applied Bacteriology

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Section: Resuscitation or Pre-enrichment Conditionssupporting

confidence: 86%

Section: Enrichment Mediamentioning

confidence: 99%

Section: Enrichment Mediamentioning

confidence: 99%

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The isolation of salmonellas and campylobacters

Fricker

1987

Journal of Applied Bacteriology

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“…One reason RV is favored in enrichment protocols is that the combination of inhibitors and the lower nutrient concentration reduces the numbers of competing bacteria such as Proteus that might be present in some samples [7]. The addition of soya peptone to RV was shown to increase the growth of Salmonella and give slightly better recovery of Salmonella in general [25]. In the present study RVS provided a more equivalent distribution of all of the strains than did RV for the mixtures in both the Salmonella -only and the added fecal bacteria enrichments.…”

Section: Discussionmentioning

confidence: 99%

Selective Enrichment Media Bias the Types of Salmonella enterica Strains Isolated from Mixed Strain Cultures and Complex Enrichment Broths

Gorski

2012

PLoS ONE

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For foodborne outbreak investigations it can be difficult to isolate the relevant strain from food and/or environmental sources. If the sample is contaminated by more than one strain of the pathogen the relevant strain might be missed. In this study mixed cultures of Salmonella enterica were grown in one set of standard enrichment media to see if culture bias patterns emerged. Nineteen strains representing four serogroups and ten serotypes were compared in four-strain mixtures in Salmonella-only and in cattle fecal culture enrichment backgrounds using Salmonella enrichment media. One or more strain(s) emerged as dominant in each mixture. No serotype was most fit, but strains of serogroups C2 and E were more likely to dominate enrichment culture mixtures than strains of serogroups B or C1. Different versions of Rappaport-Vassiliadis (RV) medium gave different patterns of strain dominance in both Salmonella-only and fecal enrichment culture backgrounds. The fittest strains belonged to serogroups C1, C2, and E, and included strains of S. Infantis, S. Thompson S. Newport, S. 6,8:d:-, and S. Give. Strains of serogroup B, which included serotypes often seen in outbreaks such as S. Typhimurium, S. Saintpaul, and S. Schwarzengrund were less likely to emerge as dominant strains in the mixtures when using standard RV as part of the enrichment. Using a more nutrient-rich version of RV as part of the protocol led to a different pattern of strains emerging, however some were still present in very low numbers in the resulting population. These results indicate that outbreak investigations of food and/or other environmental samples should include multiple enrichment protocols to ensure isolation of target strains of Salmonella.

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“…In comparisons of the efficiency of RV medium containing soya peptone (RV-soya) with that of the standard RV medium, we have demonstrated that RV-soya is a marginally better medium (Fricker & Girdwood, 1985;McGibbon, Quail & Fricker, 1984).…”

Section: Introductionmentioning

confidence: 99%

An evaluation of commercially available dehydrated Rappaport-Vassiliadis medium for the isolation of salmonellae from poultry

Fricker¹,

Quail²,

McGibbon³

et al. 1985

J. Hyg.

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SUMMARYA total of 745 samples of chicken giblets was cultured to determine the relative efficiency of a commercially available Rappaport-Vassiliadis medium (RV-Oxoid). Experiments to determine the optimum inoculation ratio showed that 1: 100 was superior to the other ratios tested. Comparison of RV-Oxoid with standard RV and RV-medium prepared using soya peptone (RV-soya) showed that after 24 h RV-soya was significantly better than RV-Oxoid (P < 0-05), although there was no significant difference between standard RV and RV-Oxoid. Furthermore, when the duration of incubation was extended to 48 h there was no significant difference between the three media (P > 0 25).We conclude that RV-Oxoid is a satisfactory product for the isolation of salmonellae from poultry, providing that it is inoculated at a ratio of 1: 100 and is incubated for 48h. Its use can therefore be recommended to laboratories who wish to use a dehydrated medium.

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Isolation of salmonellae using two forms of Rappaport-Vassiliadis medium and brilliant green agar

Cited by 11 publications

References 20 publications

The isolation of salmonellas and campylobacters

The isolation of salmonellas and campylobacters

Selective Enrichment Media Bias the Types of Salmonella enterica Strains Isolated from Mixed Strain Cultures and Complex Enrichment Broths

An evaluation of commercially available dehydrated Rappaport-Vassiliadis medium for the isolation of salmonellae from poultry

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