Isolation of Salmonella from Diarrheic Feces of Pigs.

Asai, Tetsuo; Otagiri, Yukiko; Osumi, Takayuki; Namimatsu, Takanori; Hirai, Hidetoshi; Sato, Shizuo

doi:10.1292/jvms.64.159

Cited by 18 publications

(17 citation statements)

References 8 publications

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“…The isolation rates of Salmonella belonging to serovar O4 group were 68.1%, 58.7%, and 58.6% in the 1970s, 1980s and in 1998, respectively [7,17]. Furthermore, the isolation rates of Salmonella belong to serovar O4 group were 51.4% and 88.1% of pigs in disorder [1] or diarrhea [2], respectively. Thereby, our results in Experiment 2 may partially show the Salmonella prevalence of pigs in Japan.…”

Section: Discussionmentioning

confidence: 99%

“…Bacteriological examination: Isolation of Salmonella was attempted from fecal samples as previously described [2,8].…”

Section: Serological Surveys On the Pig Farms (Experiments 2)mentioning

confidence: 99%

“…To avoid outbreak of food-borne diseases due to meat and eggs, their practitioner and veterinarian should recognize and take care for prevention of infection of domestic animals with food-borne disease related organisms including Salmonella. Generally, to estimate the Salmonella prevalence in pig herds, bacteriological examinations were performed on fecal and clinical tissue samples of pigs [2,7,13,17]. Serological examinations by enzyme-linked immunosorbent assay (ELISA) of serum and meat juice were also undertaken in several countries [9,10,14,15].…”

mentioning

confidence: 99%

“…So, under the field conditions, we compared the serological examinations with bacteriological examinations. Furthermore, as previous reports have shown that O4 group is the dominant serovars of Salmonella infecting pigs in Japan [1,2,7,17], we conducted surveillance for the antibody prevalence in the serum samples collected from pigs in Japan.…”

mentioning

confidence: 99%

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Isolation and Serological Survey of Salmonella in Pigs in Japan.

Asai

Fujii

Osumi

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. A total of 267 fecal and serum samples collected from individual pigs reared on a Salmonella-positive farm were subjected to bacteriological and serological examinations of Salmonella. Salmonella was isolated from 47 pigs (17.6%) and prevalence of antibody to lipopolysaccharide (LPS) of S. Typhimurium, which was partly common to S. O4, 12: d: -, was observed in 90 pigs (33.7%). Salmonella was isolated from 26 (28.9%) of 90 antibody-positive pigs and 21 (11.9%) of 177 antibody-negative pigs. Twenty-one of 36 pigs (58.3%) positive for S. O4, 12: d: -, five of 10 pigs (50.0%) positive for S. Havana, and none for S. Anatum had antibodies. Thus, seropositive rates were higher than isolation-positive rates, and antibody prevalence was associated with serovars of the isolates. Then, we analyzed antibody prevalence among pigs on Japanese pig farms. The antibodies to LPS of S. Typhimurium were found in 195 of 1,498 pigs (13.0%) and in at least one serum sample on 35 of 52 farms (67.3%). Our results indicate that Salmonella does not seem to be so prevalent in pigs though it is widely prevalent among pig farms.

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Section: Discussionmentioning

confidence: 99%

“…Bacteriological examination: Isolation of Salmonella was attempted from fecal samples as previously described [2,8].…”

Section: Serological Surveys On the Pig Farms (Experiments 2)mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Isolation and Serological Survey of Salmonella in Pigs in Japan.

Asai

Fujii

Osumi

et al. 2002

The Journal of Veterinary Medical Science

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“…Many Salmonella serovars other than S. Choleraesuis cause food poisoning in human. Previous studies have shown that several serovars of Salmonella are prevalent among pigs [2,4,6,[11][12][13]. Hence, Salmonella prevalence among pigs causes a significant health problem for pigs and for people.…”

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confidence: 99%

Enrichment for Isolating Salmonella Choleraesuis and other Salmonella spp. from Pigs

Osumi

Asai

Namimatsu

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. The growth of Salmonella Choleraesuis was examined in Rappaport Vassiliadis broth (RV) and Hajna-tetrathionate broth (HTT) at 37 and 42°C. As the enrichment in RV at 37°C was satisfactory for isolating S. Choleraesuis, we used this enrichment for isolation from the samples collected from 15 asymptomatic pigs reared on a S. Choleraesuis contaminated farm. S. Choleraesuis was frequently isolated from six pigs (40.0%) under field conditions. The isolation of other Salmonella serovars than S. Choleraesuis was attempted by using both RV enrichment at 37°C and HTT enrichment at 42°C. Salmonella organisms were isolated from 156 (44.8%) of 348 fecal samples and more frequently with HTT at 42°C (43.4%) than with RV at 37°C (20.9%). If other serovars in addition to S. Choleraesuis are to be surveyed, HTT enrichment should be used in combination with RV enrichment.

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Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs

Guo

et al. 2006

Antonie Van Leeuwenhoek

161

109

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A total of 124 samples were collected from the intestine of broiler chickens, piglet faeces, fermented foods, soils and Chinese herbs. More than 750 strains of aerobic, spore-forming bacteria were isolated from these samples. The inhibitory activity of these spore-forming strains against Escherichia coli K88, E. coli K99, Salmonella typhimurium and Staphylococcus aureus was assessed using a disc plate diffusion assay. The six bacilli with the largest inhibition zones against the four indicator bacteria were chosen and assessed for their resistance to unfavorable conditions within simulated gut environments. The strain Bacillus subtilis MA139 showed full resistance to pH 2, 0.3% bile salts and exhibited the highest antimicrobial activity. Based on these results, B. subtilis MA139 was selected as a potential probiotic and fed to piglets at concentrations of 2.2 x 10(5), 2.2 x 10(6) or 2.2 x 10(7) CFU/g of feed during a 28-day feeding trial. A negative control consisting of the basal diet with no additives and a positive control consisting of the basal diet supplemented with 16 g/ton flavomycin were also included. Ninety piglets between 35 and 40 days old were used in the in vivo animal trials. B. subtilis MA139 enhanced daily gain (P = 0.10) and feed conversion (P = 0.03) compared with the negative control. The performance of pigs fed B. subtilis MA139 supplemented diets did not differ from that of pigs fed the antibiotic diet. There was a significant increase in Lactobacilli cell counts (P = 0.02) and a numerical decrease in E. coli counts (P = 0.05) in the faecal samples of pigs fed B. subtilis MA139 with 2.2 x 10(5) CFU/g of feed. The overall results of this study show that the use of initial co-culture with indicator pathogens, a disc plate diffusion assay and simulated gut environment tolerance tests is one of effective methods of screening Bacillus for probiotic use and that B. subtilis MA139 is a promising alternative to antibiotics for use as a feed additive in piglet diets.

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Isolation of Salmonella from Diarrheic Feces of Pigs.

Cited by 18 publications

References 8 publications

Isolation and Serological Survey of Salmonella in Pigs in Japan.

Isolation and Serological Survey of Salmonella in Pigs in Japan.

Enrichment for Isolating Salmonella Choleraesuis and other Salmonella spp. from Pigs

Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs

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