1986
DOI: 10.1111/j.1399-3054.1986.tb02443.x
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Isolation of ribulose bisphosphate carboxylase‐oxygenase from non‐hardened and hardened needles of Pinus sylvestris

Abstract: Ribulose bisphosphate carboxylase‐oxygenase, RuBP carboxylase (EC 4.1.1.39), was purified from non‐hardened and hardened needles of Pinus sylvestris L. Needles were collected from pine seedlings cultivated in nutrient solution in a climate chamber from seedlings grown outdoors, and from a tree in a natural stand. The enzyme was isolated from crude extracts through quantitative precipitation in polyethylene glycol 4000 and MgCl2, followed by sucrose gradient centrifugation in a fixed angle rotor. The purified e… Show more

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Cited by 7 publications
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“…The slurry was filtered and centrifuged (40000g, 20min). The enzyme in the supernatant was purified, by sucrose gradient centrifugation, and quantified as described in (Gezelius and Widell 1986) except for the medium used: 0.05 M Tris-HC1-N a O H (pH 8 at 4 °C), 0.05 M NaHCO3,0.01 M MgC12, 1 mM EDTA, 1 mM fl-mercaptoethanol. The working range of the plates was defined with three lots of the standard (precipitate heights about 2.5, 3.2 and 4.2 cm) that in all cases could be fitted to a line with r 2 > 0.999.…”
Section: Methodsmentioning
confidence: 99%
“…The slurry was filtered and centrifuged (40000g, 20min). The enzyme in the supernatant was purified, by sucrose gradient centrifugation, and quantified as described in (Gezelius and Widell 1986) except for the medium used: 0.05 M Tris-HC1-N a O H (pH 8 at 4 °C), 0.05 M NaHCO3,0.01 M MgC12, 1 mM EDTA, 1 mM fl-mercaptoethanol. The working range of the plates was defined with three lots of the standard (precipitate heights about 2.5, 3.2 and 4.2 cm) that in all cases could be fitted to a line with r 2 > 0.999.…”
Section: Methodsmentioning
confidence: 99%