Isolation of potential probiotic Lactobacillus oris HMI68 from mother's milk with cholesterol-reducing property

Anandharaj, Marimuthu; Sivasankari, B.

doi:10.1016/j.jbiosc.2014.01.015

Cited by 69 publications

(57 citation statements)

References 41 publications

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“…Inhibition was not observed in E. faecalis by L. animalis FR12 whereas B. tequilensis FR9 strain showed broadest antimicrobial spectrum against all tested pathogens with the maximum inhibitory effect on L. monocytogenes (3250 AU/mL). Similar kind of results has been reported previously by various authors (Ahn et al, 2002; Strompfova and Laukova, 2007; Argyri et al, 2013; Anandharaj and Sivasankari, 2014; Khochamit et al, 2015). This is the first study reporting a broad spectrum antimicrobial activity of B. tequilensis FR9.…”

Section: Resultssupporting

confidence: 92%

“…Coetzee (2015) reported that B. tequilensis 5A2 was resistant to penicillin, tetracycline, streptomycin, and trimethoprim. Antibiotic resistance of Lactobacillus and Bacillus strains were also reported by other authors (Messaoudi et al, 2012; Anandharaj and Sivasankari, 2014; Nguyen et al, 2015). …”

Section: Resultssupporting

confidence: 80%

“…Bacteria predestined for probiotic use should be screened for antibiotic resistance to prevent any potential transfer of undesirable antibiotic resistance into the intestinal niche (Anandharaj and Sivasankari, 2014). Hence, probiotics strains were subjected to antibiotic susceptibility tests using eleven antibiotics.…”

Section: Resultsmentioning

confidence: 99%

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Purification of Antilisterial Peptide (Subtilosin A) from Novel Bacillus tequilensis FR9 and Demonstrate Their Pathogen Invasion Protection Ability Using Human Carcinoma Cell Line

et al. 2016

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This study focuses on isolation, screening, and characterization of novel probiotics from gastrointestinal tract of free-range chicken (Gallus gallus domesticus). Fifty seven colonies were isolated and three isolates (FR4, FR9, and FR12) were selected and identified as Lactobacillus gasseri FR4, Bacillus tequilensis FR9, and L. animalis FR12 by 16S rRNA sequencing. Three strains were able to survive in stimulated acidic and bile conditions and inhibit the growth of pathogens. Especially, FR9 exhibited maximum inhibition against Listeria monocytogenes and none of them exhibited hemolytic activity. Native-PAGE revealed the presence of low molecular weight (3.4–5.0 KDa) antimicrobial peptide. The peptide was further purified by Sephadex G-50 column and RP-HPLC using C18 column. N-terminal amino acid sequencing of antimicrobial peptide showed 100% consensus to antilisterial peptide Subtilosin A and SboA gene was amplified from FR9 genome. FR9 showed maximum aggregation activity, exopolysaccharide production (85.46 mg/L) and cholesterol assimilation (63.12 ± 0.05 μg/mL). Strong adhesion property (12.6%) and pathogen invasion protection ability was revealed by B. tequilensis FR9 towards HCT-116 human colon carcinoma cell line. This is the first study to demonstrate antilisterial Subtilosin A production of B. tequilensis. Our results indicate that B. tequilensis FR9 strain furnish the essential characteristics of a potential probiotics and might be incorporated into human and animal food supplements.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 80%

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Purification of Antilisterial Peptide (Subtilosin A) from Novel Bacillus tequilensis FR9 and Demonstrate Their Pathogen Invasion Protection Ability Using Human Carcinoma Cell Line

et al. 2016

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“…112 Furthermore, cholesterol-reducing properties were also observed for L. oris HMI118, HMI28, HMI43, HMI68 and HMI74 isolated from breast milk. 113 Although all the tested strains assimilated cholesterol even in the absence of bile salts, surviving in the acidic conditions of the intestine and tolerating high bile concentrations, L. oris HMI68 showed the highest cholesterol assimilation deconjugating sodium glycocholate (the most predominant bile salt in the human intestine) and sodium taurocholate. Table 4 Clinical studies of lactobacilli showing efficacy for treatment of hypercholesterolaemia Cholesterol assimilation has also been evaluated as a possible therapeutic approach to reduce the risk for cardiovascular diseases.…”

Section: Cholesterol-lowering Activitymentioning

confidence: 92%

Mechanisms and therapeutic effectiveness of lactobacilli

et al. 2015

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The gut microbiome is not a silent ecosystem but exerts several physiological and immunological functions. For many decades, lactobacilli have been used as an effective therapy for treatment of several pathological conditions displaying an overall positive safety profile. This review summarises the mechanisms and clinical evidence supporting therapeutic efficacy of lactobacilli. We searched Pubmed/Medline using the keyword ‘Lactobacillus’. Selected papers from 1950 to 2015 were chosen on the basis of their content. Relevant clinical and experimental articles using lactobacilli as therapeutic agents have been included. Applications of lactobacilli include kidney support for renal insufficiency, pancreas health, management of metabolic imbalance, and cancer treatment and prevention. In vitro and in vivo investigations have shown that prolonged lactobacilli administration induces qualitative and quantitative modifications in the human gastrointestinal microbial ecosystem with encouraging perspectives in counteracting pathology-associated physiological and immunological changes. Few studies have highlighted the risk of translocation with subsequent sepsis and bacteraemia following probiotic administration but there is still a lack of investigations on the dose effect of these compounds. Great care is thus required in the choice of the proper Lactobacillus species, their genetic stability and the translocation risk, mainly related to inflammatory disease-induced gut mucosa enhanced permeability. Finally, we need to determine the adequate amount of bacteria to be delivered in order to achieve the best clinical efficacy decreasing the risk of side effects.

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“…Bile reduces the number of bacteria by destroying their cell walls. The concentration of bile salts in the human body is 0.3-0.5% [18][19][20], and several probiotic bacteria have been shown to survive these concentrations [21], found Lactobacillus oris HM168 isolated from breast milk, survives at 0.3% and 0.5% bile salt for 5 h. Likewise, the strain of lactic acid bacteria isolated from buffalo milk from Karnataka, India, was able to survive in concentrations of 0.3% and 0.5% bile salts, but this was not sustained at 1% [2]. The five isolates of lactic acid bacteria were tested for bile salt resistance of 0.3% and 0.5%, for 5 h. Fig.…”

Section: Lactic Acid Bacteria Resistance Against Bile Saltsmentioning

confidence: 99%

Selection of Buffalo Milk Lactic Acid Bacteria With Probiotic Potential

Melia

Yuherman

Jaswandi

et al. 2018

Asian J Pharm Clin Res

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Objective: The aim of this research was to isolate and identify lactic acid bacteria using 16S rRNA and evaluates their potential as probiotics. Methods:The probiotic properties measured were resistance to low pH and to 0.3% and 0.5% bile salts, antimicrobial activity against pathogenic bacteria (Escherichia coli O157:H7 and Staphylococcus aureus ATCC 25923), antibiotic resistance, and hydrophobicity. Results:The lactic acid bacteria with optimal probiotic properties were isolated from buffalo milk and identified from a sample from Agam district (BMA 3.3) which was classified using BLAST analysis as a strain of Lactobacillus fermentum (L23). Conclusion:Buffalo milk from this part of West Sumatera contains a strain of L. fermentum with has good probiotic properties.

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Isolation of potential probiotic Lactobacillus oris HMI68 from mother's milk with cholesterol-reducing property

Cited by 69 publications

References 41 publications

Purification of Antilisterial Peptide (Subtilosin A) from Novel Bacillus tequilensis FR9 and Demonstrate Their Pathogen Invasion Protection Ability Using Human Carcinoma Cell Line

Purification of Antilisterial Peptide (Subtilosin A) from Novel Bacillus tequilensis FR9 and Demonstrate Their Pathogen Invasion Protection Ability Using Human Carcinoma Cell Line

Mechanisms and therapeutic effectiveness of lactobacilli

Selection of Buffalo Milk Lactic Acid Bacteria With Probiotic Potential

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