Isolation of potato proteins using simulated moving bed technology

Abstract

The simulated moving bed (SMB) concept of chromatography was applied to treat potato juice from production of starch. The aim was to harvest proteins. SMB offers possibilities to operate with different process strategies and in this study it was shown possible to harvest up to 80% of the protein in a process utilizing very little extra water besides that already present in the juice. After depleting protein from the juice in the adsorption step, the flow through was used to recondition the column after elution. The present study illustrates a new concept of applying chromatography as a capturing step of bulk products.

Recovery of High Value‐Added Nutrients from Fruit and Vegetable Industrial Wastewater

“…With these multiple pretreatments, a large portion of starch and fiber are removed, and 30% reduction of UF membrane fouling is achieved by MF pretreatment while at the same time increasing the purity and concentration of protein (Dabestani, Arcot, & Chen, 2017). Other studies were also conducted to recover proteins using techniques including expanded bed adsorption (EBA) (Straetkvern & Schwarz, 2012) and simulated moving bed chromatography technology (SMBC) (Andersson, Sahoo, & Mattiasson, 2008).…”

Immobilized metal affinity chromatography in open-loop simulated moving bed technology: Purification of a heat stable histidine tagged β-glucosidase

“…This has been shown in an earlier report [26]. However, due to a very small loss in activity in the flow through even by loading 100 ml it was decided to only apply sample over one column length using three parallel columns which is similar to loading on to a single column.…”

Towards potato protein utilisation: insights into separation, functionality and bioactivity of patatin

“…On a dry matter basis, PFJ contains approximately 2% (w/w) protein, which has great potential as a high value‐added food ingredient (Barta et al ., ). Due to the inappropriate processing methods during potato protein separation that lead to impaired functional properties of potato protein, PFJ has been traditionally utilised as low‐value fertiliser or animal feed, but environmental regulations may demand alternative exploitation of PFJ (Andersson et al ., ; Barta et al ., ). Therefore, there is an increasing interest in effective utilisation of PFJ in the potato starch industry.…”