Isolation of Polycyclic Aromatic Hydrocarbons (PAHs) Degrading Fungal Candidate from Oil-Contaminated Soil and Degradation Potentiality Study on Anthracene

Lahkar, Jiumoni; Deka, Hemen

doi:10.1080/10406638.2016.1220957

Cited by 7 publications

(4 citation statements)

References 20 publications

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“…Besides, each fungus needs time to adapt and finally produce intracellular and extracellular enzymes that allow it to degrade PAHs (Reyes‐César et al . 2014; Lahkar and Deka 2017). Also, is important consider that changes induced by hydrocarbons could lead to altered membrane function, alteration of cell homeostasis and alteration of energy transduction (Argumedo‐Delira et al .…”

Section: Resultsmentioning

confidence: 99%

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Analysis of phenanthrene degradation by Ascomycota fungi isolated from contaminated soil from Reynosa, Mexico

Cruz-Izquierdo

Paz-González

Reyes-Espinosa

et al. 2021

Lett Appl Microbiol

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Polycyclic aromatic hydrocarbons (PAHs) are organic compounds generated mainly by anthropogenic sources. They are considered toxic to mammals, since they have carcinogenic, mutagenic and genotoxic properties, among others. Although mycoremediation is an efficient, economical and eco‐friendly technique for degrading PAHs, the fungal degradation potential of the phylum Ascomycota has not been widely studied. In this work, we evaluated different fungal strains from the polluted soil of ‘La Escondida’ lagoon in Reynosa, Mexico to know their potential to degrade phenanthrene (PHE). Forty‐three soil isolates with the capacity to grow in the presence of PHE (0·1% w/v) were obtained. The fungi Aspergillus oryzae MF13 and Aspergillus flavipes QCS12 had the best potential to degrade PHE. Both fungi germinated and grew at PHE concentrations of up to 5000 mg l−1 and degraded 235 mg l−1 of PHE in 28 days, with and without an additional carbon source. These characteristics indicate that A. oryzae MF13 and A. flavipes QCS12 could be promising organisms for the remediation of sites contaminated with PAHs and detoxification of recalcitrant xenobiotics.

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Section: Resultsmentioning

confidence: 99%

“…Isolates without the presence of PHE were considered as controls. Fungal growth was assessed by measuring the optical density (OD) at 600 nm in a microplate absorbance reader from Bio‐Rad (iMark™ Microplate Reader) (Lahkar and Deka 2017).…”

Section: Methodsmentioning

confidence: 99%

Analysis of phenanthrene degradation by Ascomycota fungi isolated from contaminated soil from Reynosa, Mexico

Cruz-Izquierdo

Paz-González

Reyes-Espinosa

et al. 2021

Lett Appl Microbiol

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“…Anthracene quantifications were accomplished by employing an oven temperature program, which entailed an initial temperature of 140°C sustained for 2 min. After that there was a steady and gradual rise in temperature at a rate with 10°C per min until reaching 240°C, and then maintaining it for 7 min [25]. The anthracene concentrations in different samples were measured by assessing their peak area in the spectra and retention time (Rt) [14].…”

Section: Methodsmentioning

confidence: 99%

Green titanium dioxide (TiO₂) nanoparticles assisted biodegradation of anthracene employing Serratia quinivorans HP5

Chakravarty,

Deka,

Chowdhury

2024

J Basic Microbiol

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The anthracene biodegradation potential of Serratia quinivorans HP5 was studied under a controlled laboratory environment. The green TiO2 nanoparticles (NPs) synthesized from Paenibacillus sp. HD1PAH was used to accelerate the biodegradation process. The synergistic application of TiO2 NPs and S. quinivorans HP5 resulted in a reduction of anthracene concentration by 1.2 folds in liquid‐medium and 1.5 folds in contaminated soil. Gas‐chromatography and mass‐spectrometric investigation showed the production of four anthracene derivatives, namely 1,2‐anthracene dihydrodiol, 6,7‐benzocoumarin, anthrone, and 9,10‐anthraquinoneat the termination of experimental periods. Furthermore, bacterial biomass increased by 23.3 folds in the presence of TiO2 NPs, and overall soil enzyme activities were enhanced by 4.2 folds in the treated samples. In addition, there was a negative correlation observed between the biomass of S. quinivorans HP5 and the concentrations of anthracene, suggesting the involvement of bacterium in anthracene biodegradation processes. The degradation pathway of anthracene revealed its transformation into the less toxic compound 9,10‐anthraquinone. Overall, this study elucidates a novel biodegradation pathway for anthracene and highlights the potential of nano‐assisted bacterial remediation as a promising approach for environmental cleanup.

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“…Due to oil leaks from industrial complexes and petrol stations, soil and groundwater contamination are considered a severe environmental and social hazard (Zhang et al, 2006). Among these contaminants are the ubiquitous aromatic compounds such as polycyclic aromatic hydrocarbons (PAHs) that contaminate the environment because of the incomplete combustion of organic materials in addition to agricultural, domestic, and industrial processes (Cheng et al, 2016;Lahkar and Deka, 2016). PAHs encompass some of the most persistent natural pollutants belongs to PAHs, which have severe biological effects such as carcinogenicity, mutagenicity, and genotoxicity (Ledra, 2011;Cauduro et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a polycyclic aromatic hydrocarbons (PAHs) degrader, Burkholderia contaminans strain P14, isolated from aged oil-contaminated soil in Kuwait

Akbar

Rahmeh

Kishk

et al. 2022

Appl Env Biotechnol

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Soil contamination by polycyclic aromatic hydrocarbons (PAHs) and heavy metals is a major concern affecting soil quality. Bioremediation is an efficient approach to decontaminate these pollutants while posing the lowest risk to the environment. This technique is based on identifying microorganisms with the metabolic potential to degrade the pollutants. In this study, the physiochemical properties of oil-contaminated soils in Kuwait were investigated. The soil had alkaline pH (8.36) with a salinity of 0.08% and PAHs compounds were detected at high concentrations (pyrene, 1212 mg/kg), (phenanthrene, 710 mg/kg) and (fluorene 326 mg/kg). Heavy metals such as iron, aluminum, sodium, magnesium, vanadium, copper, etc., were also detected in the soil samples. Then, selective screening for PAH degraders was performed and assessed by colony forming units (CFU) and 16S rDNA gene sequences to determine their growth profiles and taxonomical identification. In total, 21 strains were selected for their resistance to PAHs, which include Pseudomonas (9), Burkholderia (6), Bacillus (2), Bordetella (1), Microbacterium (1), Micrococcus (1), and Kocuria (1). Among all, the growth by Burkholderia sp. P14, in the presence of phenanthrene and fluorene, has maintained a stationary phase from day 5 to 8 with a 6 log CFU/ml bacterial count. The draft genome of the Burkholderia contaminans P14 strain comprised 68 contigs with 8,584,157bp, 66% GC content, 4 rRNA and 75 tRNA. A total of 80 genes were involved in the degradation of the benzoate, naphthalene, and PAH . Genes that encode the PAH degradation were clustered into four distinct groups, including pcaHG, pcaB, pcaIJ, and pcaKFR and were found to be in contigs 5, 11, 8 and 13 of B. contaminans P14, respectively. KEGG analysis suggested that PAHs were degraded in P14 via the protocatechuate and catechol branches of the β-ketoadipate protocatechuate degradation pathway. The genomic island regions in P14 differed from those in the reference genome of B. contaminans M14, indicating the novelty and genomic recombination of the strain. Genomic information on P14 has helped clarify the molecular mechanisms involved in PAH catabolism. Burkholderia contaminans P14 strain will enhance the bioremediation of oil-contaminated soils.

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Isolation of Polycyclic Aromatic Hydrocarbons (PAHs) Degrading Fungal Candidate from Oil-Contaminated Soil and Degradation Potentiality Study on Anthracene

Cited by 7 publications

References 20 publications

Analysis of phenanthrene degradation by Ascomycota fungi isolated from contaminated soil from Reynosa, Mexico

Analysis of phenanthrene degradation by Ascomycota fungi isolated from contaminated soil from Reynosa, Mexico

Green titanium dioxide (TiO₂) nanoparticles assisted biodegradation of anthracene employing Serratia quinivorans HP5

Molecular characterization of a polycyclic aromatic hydrocarbons (PAHs) degrader, Burkholderia contaminans strain P14, isolated from aged oil-contaminated soil in Kuwait

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Isolation of Polycyclic Aromatic Hydrocarbons (PAHs) Degrading Fungal Candidate from Oil-Contaminated Soil and Degradation Potentiality Study on Anthracene

Cited by 7 publications

References 20 publications

Analysis of phenanthrene degradation by Ascomycota fungi isolated from contaminated soil from Reynosa, Mexico

Analysis of phenanthrene degradation by Ascomycota fungi isolated from contaminated soil from Reynosa, Mexico

Green titanium dioxide (TiO2) nanoparticles assisted biodegradation of anthracene employing Serratia quinivorans HP5

Molecular characterization of a polycyclic aromatic hydrocarbons (PAHs) degrader, Burkholderia contaminans strain P14, isolated from aged oil-contaminated soil in Kuwait

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Green titanium dioxide (TiO₂) nanoparticles assisted biodegradation of anthracene employing Serratia quinivorans HP5