Isolation of plant-growth-promoting and metal-resistant cultivable bacteria from Arthrocnemum macrostachyum in the Odiel marshes with potential use in phytoremediation

Navarro‐Torre, Salvadora; Mateos‐Naranjo, Enrique; Caviedes, Miguel A.; Pajuelo, Eloísa; Rodríguez‐Llorente, Ignacio D.

doi:10.1016/j.marpolbul.2016.06.070

Cited by 67 publications

(68 citation statements)

References 57 publications

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“…Contrarily, growth on MacConkey agar and cetrimide agar was not observed. According to Navarro-Torre et al [15], strain RA15 T is positive for the hydrolysis of starch, casein, Tween 80, DNA, chitin and pectin. Here, positive results for gelatin and aesculin hydrolysis were also observed.…”

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confidence: 99%

“…It hydrolyses substrates such as starch, casein, Tween 80, DNA, chitin and pectin [15]. Furthermore, strain RA15 T demonstrated the ability to grow in high concentrations of heavy metals reaching values of 12 mM As and 3 mM Cu [15]. It also presented several plant growth-promoting (PGP) properties such as production of auxins and siderophores and nitrogen fixation, observing such traits even in presence of heavy metals, conditions under which higher expression of PGP traits were favoured [15].…”

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confidence: 99%

“…This report aims to elucidate the taxonomic status of strain RA15 T , a strain showing biotechnological potential in the phytostabilization of heavy metal-polluted soils, in the genus Pseudoalteromonas following a polyphasic approach. RA15 T was isolated from the rhizosphere of A. macrostachyum from the Odiel marshes (37° 13′ N, 6° 57′ W) as described in the work of Navarro-Torre et al [15]. Succinctly, rhizosphere samples were mixed with sterile saline solution (0.9 %, w/v) and then shaken for 5 min.…”

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confidence: 99%

“…Growth conditions were determined incubating the strain on TSA 2.25 % NaCl (w/v). The range of temperature for growth was tested at 4,15,20,25,28,30,32,37 and 45 °C for 6 days. The range of pH for growth was assessed at pH 5.0, 6.0, 7.0, 8.0 and 9.0 for 6 days.…”

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confidence: 99%

“…Moreover cells of RA15 T were fixed with 2 % uranyl acetate and morphology was observed using a transmission electron microscope (Libra 120, Zeiss). Motility was tested by incubating the strain in tryptic soy broth (TSB) supplemented with 2.5 % NaCl (w/v) at 28 °C for 30 min [15]. After that, a drop from the culture was observed under optical microscope with a ×40 objective.…”

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Pseudoalteromonas rhizosphaerae sp. nov., a novel plant growth-promoting bacterium with potential use in phytoremediation

Navarro‐Torre

Carro

Rodríguez‐Llorente

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Strain RA15T was isolated from the rhizosphere of the halophyte plant Arthrocnemum macrostachyum growing in the Odiel marshes (Huelva, Spain). RA15T cells were Gram stain-negative, non-spore-forming, aerobic rods and formed cream-coloured, opaque, mucoid, viscous, convex, irregular colonies with an undulate margin. Optimal growth conditions were observed on tryptic soy agar (TSA) plates supplemented with 2.5 % NaCl (w/v) at pH 7.0 and 28 °C, although it was able to grow at 4–32 °C and at pH values of 5.0–9.0. The NaCl tolerance range was from 0 to 15 %. The major respiratory quinone was Q8 but Q9 was also present. The most abundant fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C17 : 1 ω8c and C16 : 0. The polar lipids profile comprised phosphatidylglycerol and phosphatidylethanolamine as the most abundant representatives. Phylogenetic analyses confirmed the well-supported affiliation of strain RA15T within the genus Pseudoalteromonas , close to the type strains of Pseudoalteromonas neustonica , Pseudoalteromonas prydzensis and Pseudoalteromonas mariniglutinosa . Results of comparative phylogenetic and phenotypic studies between strain RA15T and its closest related species suggest that RA15T could be a new representative of the genus Pseudoalteromonas , for which the name Pseudoalteromonas rhizosphaerae sp. nov. is proposed. The type strain is RA15T (=CECT 9079T=LMG 29860T). The whole genome has 5.3 Mb and the G+C content is 40.4 mol%.

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Pseudoalteromonas rhizosphaerae sp. nov., a novel plant growth-promoting bacterium with potential use in phytoremediation

Navarro‐Torre

Carro

Rodríguez‐Llorente

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Kushneria

Ventosa

Sánchez‐Porro

2020

Bergey's Manual of Systematics of Archaea and Bacteria

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Kush.ne'ri.a. N.L. fem. n. Kushneria , from the name Kushner, honoring Dr. Donn J. Kushner, a Canadian microbiologist who carried out pioneering studies on halophilic microorganisms. Proteobacteria / Gammaproteobacteria / Oceanospirillales / Halomonadaceae / Kushneria The genus Kushneria is classified within the family Halomonadaceae and the order Oceanospirillales , in the class Gammaproteobacteria . The cells are Gram‐stain‐negative, motile, and non‐endospore‐forming rods. Colonies are orange, light orange, light salmon, or yellow pigmented. Chemoorganotrophic. Aerobic or facultatively anaerobic. Catalase‐positive and oxidase‐negative. Optimal growth at 0.5–12% (w/v) NaCl, at pH 7.0–9.0, and 25–37°C. The major respiratory quinone is ubiquinone 9 (Q9). The predominant cellular fatty acids are C 16:0 , C 18:1 ω7 c , C 19:0 cyclo ω 8c , and C 12:0 3‐OH. The polar lipids are phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids, two glycolipids, and an aminoglycolipid. The DNA G + C content is 59.0–64.1 mol%. Currently, the genus includes nine species: Kushneria aurantia (type species of the genus), Kushneria avicenniae , Kushneria endophytica , Kushneria indalinina , Kushneria konosiri , Kushneria marisflavi , Kushneria pakistanensis , Kushneria phyllosphaerae , and Kushneria sinocarnis . The strains of these species were isolated from halophyte plants, salterns and saline soils, marine habitats, and fermented food. DNA G + C content (mol%) : 59.0–64.1 ( T m /genome). Type species : Kushneria aurantia Sánchez‐Porro et al. 2009 VP .

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Biofilm and metallothioneins: A dual approach to bioremediate the heavy metal menace

Roy,

Maitra,

Sarkar

et al. 2023

Environmental Quality Mgmt

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Metallothioneins are a class of proteins produced by both prokaryotes and eukaryotes, having low molecular weight and abundant cysteine residues. These proteins play a humongous role in binding, sequestration, and even buffering of the intracellular metal ions. Though they are a wide class of proteins, much of them are yet to be explored. Their metal binding attribute is unique and they form distinctive metal‐thiolate clusters. They have also been known to have ROS scavenging activities due to the presence of the cysteine residues. Phytochelatins also play major roles in metal sequestration pathways. Biofilms on the other hand are clusters of bacterial cells surrounded by an extracellular matrix of polymeric substances secreted by the bacteria themselves. Biofilms play multiple roles, from nutrient sequestration, stress resistance to surface adherence. But lesser explored arenas include assistance in heavy metal trapping and bio‐remediation. Researchers have conducted studies that have demonstrated increased metal trapping, resistance and uptake in biofilm forming strains than non‐biofilm forming mutants. Therefore, this study would explore the dual role of metallothionein and biofilm in their activity of metal sequestration and heavy metal remediation and provide certain insights so as to keenly understand the correlations between the two.

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Isolation of plant-growth-promoting and metal-resistant cultivable bacteria from Arthrocnemum macrostachyum in the Odiel marshes with potential use in phytoremediation

Cited by 67 publications

References 57 publications

Pseudoalteromonas rhizosphaerae sp. nov., a novel plant growth-promoting bacterium with potential use in phytoremediation

Pseudoalteromonas rhizosphaerae sp. nov., a novel plant growth-promoting bacterium with potential use in phytoremediation

Kushneria

Biofilm and metallothioneins: A dual approach to bioremediate the heavy metal menace

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