Isolation of phosphate-solubilizing bacteria from subtropical soils with different fertilization histories

Matter, Juliana Maria; Sampaio, Sílvio César; Rosa, Danielle Medina; Remor, Marcelo Bevilacqua; Model, Kathleen Jeniffer; Sbizzaro, Mariana

doi:10.14393/bj-v36n3a2020-47955

Cited by 6 publications

(5 citation statements)

References 15 publications

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“…In fact, calcium-bound phosphate, as in the case of AP, has greater solubility than phosphate bound to Fe or Al ( Viégas et al, 2010 ). This pattern of greater solubilization of calcium phosphate mediated by microorganisms in vitro assays has been observed previously ( Silva et al, 2014 ; Spagnoletti et al, 2017 ; Matter et al, 2020 ).…”

Section: Discussionsupporting

confidence: 85%

Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo

et al. 2021

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Rock phosphate (RP) is a natural source of phosphorus for agriculture, with the advantage of lower cost and less impact on the environment when compared to synthetic fertilizers. However, the release of phosphorus (P) from RP occurs slowly, which may limit its short-term availability to crops. Hence, the use of P-solubilizing microorganisms to improve the availability of P from this P source is an interesting approach, as microorganisms often perform other functions that assist plant growth, besides solubilizing P. Here, we describe the characterization of 101 bacterial isolates obtained from the rhizosphere and endosphere of maize plants for their P solubilizing activity in vitro, their growth-promoting activity on millet plants cultivated in soil amended with RP, and their gene content especially associated with phosphate solubilization. For the in vitro solubilization assays, two mineral P sources were used: rock phosphate from Araxá (Brazil) mine (AP) and iron phosphate (Fe-P). The amounts of P released from Fe–P in the solubilization assays were lower than those released from AP, and the endophytic bacteria outperformed the rhizospheric ones in the solubilization of both P sources. Six selected strains were evaluated for their ability to promote the growth of millet in soil fertilized with a commercial rock phosphate (cRP). Two of them, namely Bacillus megaterium UFMG50 and Ochrobactrum pseudogrignonense CNPMS2088, performed better than the others in the cRP assays, improving at least six physiological traits of millet or P content in the soil. Genomic analysis of these bacteria revealed the presence of genes related to P uptake and metabolism, and to organic acid synthesis. Using this approach, we identified six potential candidates as bioinoculants, which are promising for use under field conditions, as they have both the genetic potential and the experimentally demonstrated in vivo ability to improve rock phosphate solubilization and promote plant growth.

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Section: Discussionsupporting

confidence: 85%

Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo

et al. 2021

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“…In fact, the characteristics observed in the soil directly interfere with the functional traits expressed by the resident microbiota (ROCHA et al, 2020). MATTER et al (2020); however, evaluated the The reduction in pH of the culture medium by the isolates correlates negatively with increasing levels of soluble P. These data corroborated with those observed by other authors (MONROY et al, 2020;SILVA et al, 2021) since medium acidification constitutes an important mechanism associated with P availability (BOLAN et al, 1994;MARDAD et al, 2013;MARRA et al, 2015). The correlations observed; however, were moderate.…”

Section: Soil Attributes Of Soybean Cultivation Areas Select Function...supporting

confidence: 84%

Plant growth-promoting rhizobacteria isolated from cultivated soils using Glycine max L. plants as bait

Fiuza,

Vitorino,

Silva

et al. 2024

Cienc. Rural

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The current production of major crops, such as Glycine max L., has become increasingly adept on the use of bio-inputs, such as application of plant growth-promoting inocula. However, the evaluation of the potential of bacterial isolates from soils with different time histories of agricultural use are still scarce, and methods for isolation and testing of multifunctional microorganisms need to be continuously innovated and improved. Thus, we used G. max bait plants to attract rhizobacteria from soils obtained from three areas with different time histories of soybean cultivation. We evaluated the influence of the management and characteristics of soils on the phosphate-solubilizing bacterial population. We then tested the multifunctional potential of the isolated rhizobacteria for calcium phosphate (CaHPO4) and iron phosphate (FePO4) solubilization, considering their potential as microbial inoculants in the future. The use of bait plants enabled the isolation of 139 phosphate solubilizing rhizobacteria, including four strains with multifunctional potential. The largest number of solubilizing bacteria was obtained from the interaction of bait plants with soil from an area with a history of soybean cultivation for thirty consecutive years. The high concentration of P, Fe, and K found in the soil were associated with this occurrence. In the in vitro tests, the pH values of the culture media had a moderate negative relationship with the amounts of P made available by the isolates, indicating that other processes besides the availability of organic acids, may underlie the solubilizing action of the isolates. The rhizobacteria SAF9 (Brevibacillus sp.), SAF11 (Brevibacillus sp.), BRC11 (Pseudomonas fluorescens), and SAC36 (Bacillus velezensis) stood out as multifunctional and are indicated within a perspective of obtaining bioinoculants to promote plant growth directly, indirectly, or synergistically, contributing to increase the range of bio-inputs for soybean cultivation and more sustainable agricultural practices.

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“…In fact, calcium-bound phosphate, as in the case of AP, has greater solubility than phosphate bound to Fe or Al (Viégas et al, 2010). This pattern of greater solubilization of calcium phosphate mediated by microorganisms in vitro assays has been observed previously (Silva et al, 2014;Spagnoletti et al, 2017;Matter et al, 2020). Microbacterium sp.…”

Section: Enrichment Of Bacterial Taxa From Soil Rhizospheric Cultivatsupporting

confidence: 58%

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Isolation of phosphate-solubilizing bacteria from subtropical soils with different fertilization histories

Cited by 6 publications

References 15 publications

Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo

Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo

Plant growth-promoting rhizobacteria isolated from cultivated soils using Glycine max L. plants as bait

Table_1.pdf

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