Isolation of phenoxy herbicide‐degrading <i>Rhodoferax</i> species from contaminated building material

Ehrig, A.; Müller, Roland; Babel, Wolfgang

doi:10.1002/abio.370170411

Cited by 31 publications

(31 citation statements)

References 14 publications

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“…However, our finding that bacteria belonging to the Comamonadaceae, Sphingomonadales, and Sphingomonas were enriched matches well with previous reports on phenoxypropionate herbicide degraders. Within the family of Comamonadaceae, the genera Delftia and Rhodoferax have been confirmed to be degraders (23,42). Within the order Sphingomonadales, degraders have also been found among the members of the genera Sphingomonas (46) and Sphingobium (43).…”

Section: Discussionmentioning

confidence: 86%

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Establishment of Bacterial Herbicide Degraders in a Rapid Sand Filter for Bioremediation of Phenoxypropionate-Polluted Groundwater

Feld

Nielsen

Hansen

et al. 2016

Appl Environ Microbiol

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In this study, we investigated the establishment of natural bacterial degraders in a sand filter treating groundwater contaminated with the phenoxypropionate herbicides (RS)-2-(4-chloro-2-methylphenoxy)propanoic acid (MCPP) and (RS)-2-(2,4-dichlorophenoxy)propanoic acid (DCPP) and the associated impurity/catabolite 4-chlorophenoxypropanoic acid (4-CPP). A pilot facility was set up in a contaminated landfill site. Anaerobic groundwater was pumped up and passed through an aeration basin and subsequently through a rapid sand filter, which is characterized by a short residence time of the water in the filter. For 3 months, the degradation of DCPP, MCPP, and 4-CPP in the sand filter increased to 15 to 30% of the inlet concentration. A significant selection for natural bacterial herbicide degraders also occurred in the sand filter. Using a most-probable-number (MPN) method, we found a steady increase in the number of culturable phenoxypropionate degraders, reaching approximately 5 ؋ 10 5 degraders per g sand by the end of the study. Using a quantitative PCR targeting the two phenoxypropionate degradation genes, rdpA and sdpA, encoding stereospecific dioxygenases, a parallel increase was observed, but with the gene copy numbers being about 2 to 3 log units higher than the MPN. In general, the sdpA gene was more abundant than the rdpA gene, and the establishment of a significant population of bacteria harboring sdpA occurred faster than the establishment of an rdpA gene-carrying population. The identities of the specific herbicide degraders in the sand filter were assessed by Illumina MiSeq sequencing of 16S rRNA genes from sand filter samples and from selected MPN plate wells. We propose a list of potential degrader bacteria involved in herbicide degradation, including representatives belonging to the Comamonadaceae and Sphingomonadales.G roundwater is a valuable resource that is used for drinking water in many countries all over the world. The quality of the water is vital to our health, and regulations have been made to control the concentration of contaminants and protect the groundwater (1). A major concern is leaching of pesticides mainly from agricultural areas but also from urban areas or from point sources (2). In the European Union, a legal threshold limit has been set at 0.1 g/liter for any given pesticide or 0.5 g/liter for a total concentration of mixed pesticides in drinking water (3).In Denmark, monitoring of the groundwater status has detected pesticides in almost 40% of the investigated wells, and the concentrations exceed the allowed threshold limit in 12% of the wells (4). Phenoxypropionate herbicides, including the compounds (RS)-2-(2,4-dichlorophenoxy)propanoic acid (known as DCPP or dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propanoic acid (known as MCPP or mecoprop), are among the pesticides frequently found in contaminated wells (4). The herbicides are, in particular, a problem in old landfill areas, from where they are leaching into the groundwater as a point source contamination (5). Phe...

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Section: Discussionmentioning

confidence: 86%

“…These included a Rhodoferax sp. (42) and Delftia acidovorans (24) isolated from herbicide-contaminated building rubble and Sphingobium herbicidovorans (43), an Alcaligenes sp. and a Ralstonia sp.…”

Section: Discussionmentioning

confidence: 99%

Establishment of Bacterial Herbicide Degraders in a Rapid Sand Filter for Bioremediation of Phenoxypropionate-Polluted Groundwater

Feld

Nielsen

Hansen

et al. 2016

Appl Environ Microbiol

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“…Moreover, a molecular weight of 32 kD is found with the canonical TfdA protein from R. eutropha JMP134 [2], cleaving 2,4-D with high specificity. The application of primers, derived from conserved regions of tfdA genes [7], did not result in a specific amplification product in PCR with the genome of strain MC1 [12,16] and strain MH as the template. This might be expected in agreement with the different substrate specificity found with the various strains (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…MH [10,11], Rhodoferax sp. P230 [12] and Delftia (Comamonas) acidovorans MC1 [13]. Remarkably, these strains utilise a broader spectrum of phenoxy herbicides; in addition to their ability of degrading the racemic phenoxypropionates, they similarly attack phenoxyacetate derivatives.…”

Section: Introductionmentioning

confidence: 99%

Purification and Characterisation of the Enantiospecific Dioxygenases from Delftia acidovorans MC1 Initiating the Degradation of Phenoxypropionate and Phenoxyacetate Herbicides

Westendorf

Müller

Babel

2003

Acta Biotechnologica

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SummaryAfter cultivation on (R,S)-2-(2,4-dichlorophenoxy)propionate, two α-ketoglutarate-dependent dioxygenases were isolated and purified from Delftia acidovorans MC1, catalysing the cleavage of the ether bond of various phenoxyalkanoate herbicides. One of these enzymes showed high specificity for the cleavage of the R-enantiomer of substituted phenoxypropionate derivatives: the K m values were 55 µM and 30 µM, the k cat values 55 min -1 and 34 min -1 with (R)-2-(2,4-dichlorophenoxy)propionate [(R)-2,4-DP] and (R)-2-(4-chloro-2-methylphenoxy)propionate, respectively. The other enzyme predominantly utilised the S-enantiomers with K m values of 49 µM and 22 µM, and k cat values of 50 min -1 and 46 min -1 with (S)-2-(2,4-dichlorophenoxy)propionate [(S)-2,4-DP] and (S)-2-(4-chloro-2-methylphenoxy)propionate, respectively. In addition, it cleaved phenoxyacetate herbicides (i.e. 2,4-dichlorophenoxyacetate: K m = 123 µM, k cat = 36 min -1 ) with significant activity. As the second substrate, only α-ketoglutarate served as an oxygen acceptor for both enzymes. The enzymes were characterised by excess substrate inhibition kinetics with apparent K i values of 3 mM with (R)-2,4-DP and 1.5 mM with (S)-2,4-DP. The reaction was strictly dependent on the presence of Fe 2+ and ascorbate; other divalent cations showed inhibitory effects to different extents. Activity was completely extinguished within 2 min in the presence of 100 µM diethylpyrocarbonate (DEPC).

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“…strains Sphingomonas herbicidovorans MH, 8,9) Rhodoferax sp. P230 10) and Delftia acidovorans MC1. 11) Nevertheless, these compounds still accumulate in the environment.…”

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confidence: 99%

Delftia acidovoransMC1 Resists High Herbicide Concentrations — a Study of Nutristat Growth on (RS)-2-(2,4-Dichlorophenoxy)propionate and 2,4-Dichlorophenoxyacetate

Müller¹,

Babel²

2004

Bioscience, Biotechnology, and Biochemistry

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Isolation of phenoxy herbicide‐degrading Rhodoferax species from contaminated building material

Cited by 31 publications

References 14 publications

Establishment of Bacterial Herbicide Degraders in a Rapid Sand Filter for Bioremediation of Phenoxypropionate-Polluted Groundwater

Establishment of Bacterial Herbicide Degraders in a Rapid Sand Filter for Bioremediation of Phenoxypropionate-Polluted Groundwater

Purification and Characterisation of the Enantiospecific Dioxygenases from Delftia acidovorans MC1 Initiating the Degradation of Phenoxypropionate and Phenoxyacetate Herbicides

Delftia acidovoransMC1 Resists High Herbicide Concentrations — a Study of Nutristat Growth on (RS)-2-(2,4-Dichlorophenoxy)propionate and 2,4-Dichlorophenoxyacetate

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