Isolation of phenotypically and functionally distinct macrophage subpopulations from human bronchoalveolar lavage

Spiteri, Monica A.; Clarke, S W; Poulter, L W

doi:10.1183/09031936.93.05060717

Cited by 51 publications

(1 citation statement)

References 37 publications

(35 reference statements)

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“…In contrast to many adhesive molecules which are expressed on both monocytes (the precursors of AMs in the peripheral blood) and macrophages, molecules such as CD71 (receptor for transferrin), KiM8, and 25F9 are found preferentially on mature tissue macrophages, including AMs Distinct phenotypic subpopulations of AMs have recently been identified [15,16]. Macrophages with properties of dendritic, phagocytic or suppressive cells may be separated on the basis of the eo-expression of membrane antigens RFD1, RFD7 and RFD9 [17,18].…”

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confidence: 99%

The phenotype of alveolar macrophages and its correlation with immune cells in bronchoalveolar lavage

Střı́ž¹,

Wang²,

Svarcova³

et al. 1993

Eur Respir J

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The phenotype of alveolar macrophages (AMs) is known to be modulated during pathological immune reactions in the lung. In this study, we wanted to determine the relationship between the AM phenotype and changes in the proportions of the various immune cells in bronchoalveolar lavage (BAL). BAL was performed in 76 consecutive patients, including 32 with sarcoidosis, 8 with idiopathic pulmonary fibrosis, 9 with pneumoconiosis, 13 with other respiratory disorders, and 14 controls without evidence of interstitial lung disease. The phenotype of AMs was studied by a panel of 15 monoclonal antibodies against various myeloid antigens, and was correlated with the proportions of cells obtained by BAL. The percentage of BAL lymphocytes showed a relationship with the expression of macrophage antigens in 11 out of 15 antigens studied (all except adhesive molecules CD11a, CD11c, CD18 and the antigen 25F9 present on mature macrophages). Furthermore, the CD4/CD8 ratio of BAL T-lymphocytes correlated with the AM expression of CD54 (intercellular adhesion molecule-1 (ICAM-1)), RFD1 (marker of dendritic cells), and CD71 (transferrin receptor). In samples with an increased number of bronchoalveolar neutrophils, the subpopulation of 27E10 positive AMs (inflammatory acute phase macrophages) was increased. Eosinophils in BAL were not associated with a significant increase in AM membrane antigen expression. Prominent changes of the AM phenotype were found in active sarcoidosis showing lymphocytic alveolitis, with more frequent expression of CD54, KiM2, CD71, CD11b and RFD9. In conclusion, this study shows that the phenotype of AMs is related to the type and intensity of the immunopathological reaction in the lung, and correlates with the proportions of bronchoalveolar cells.

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mentioning

confidence: 99%

The phenotype of alveolar macrophages and its correlation with immune cells in bronchoalveolar lavage

Střı́ž¹,

Wang²,

Svarcova³

et al. 1993

Eur Respir J

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Morphological aspects of particle uptake by lung phagocytes

Geiser

2002

Microscopy Res & Technique

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Macrophages residing on the inner epithelial surfaces of airways and alveoli are the only lung phagocytes exposed directly to the environment. Their phagocytic and microbicidal activities are essential for maintaining this organ in a clean and sterile state. The morphology of these phagocytes can be investigated in situ only after implementing special techniques, which involve intravascular triple-perfusion of aqueous fixatives or instillation of nonpolar ones. Such studies have revealed the engulfment of particles by these cells to be rapid, the process being essentially complete within a day. Particles are entrapped within phagosomes and the host cells eventually transported out of the lungs by mucociliary action, macrophages with higher loads being more rapidly eliminated than those with lower ones. Very small particles or those persisting on the epithelial surfaces may be taken up by the eponymous cells. Translocation of particles into the underlying connective tissue and their subsequent phagocytosis by interstitial macrophages prolongs their retention time in the lungs. The still poorly studied pleural macrophages might be involved in cell-mediated immune responses within the pleural space. Intravascular pulmonary macrophages figure largely in the phagocytosis of circulating particles. The role played by dendritic cells in particle uptake by the lungs is not well understood. Airway and alveolar macrophages are the primary phagocytes of the lung. In nonoverload situations and for particles >1 microm, a small proportion of those recruited suffices to remove material from the epithelial surface before other phagocytes, with an apparently greater immunological potential, gain access to it.

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Phenotypic and functional heterogeneity of the murine alveolar macrophage-derived cell line MH-S

Sankaran

Herscowitz

1995

Journal of Leukocyte Biology

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We have previously reported that MH-S, an established murine alveolar macrophage-derived cell line, mediated profound inhibition of in vitro antibody production, as did their freshly isolated alveolar macrophage (AM) counterparts. In this communication we show that like freshly recovered AMs, the MH-S cell line also displays phenotypic and functional heterogeneity. Sorting of parental MH-S cells by flow cytometry based on reactivity with anti-Mac-1 antibody yielded two subsets. Further analysis by staining with monoclonal antibodies against well-characterized murine macrophage cell surface markers revealed that both Mac-1+ and Mac-1- subsets expressed the mature murine macrophage antigen (F4/80) and class II major histocompatibility complex molecules, but with different intensity. In contrast, the two subsets stained equivalently with antibody against the Fc gamma II receptor, whereas neither subset stained with anti-CD4 antibody. Examination by light microscopy revealed plemorphism in the Mac-1+ population with many of the cells appearing spindle shaped and having elongated processes, whereas a majority of the cells in the Mac-1- population were spherical in shape. Functionally, cells from the Mac-1+ population were less inhibitory of in vitro antibody production and produced significantly more nitric oxide in response to stimulation with lipopolysaccharide than were cells in the Mac-1- population. Essentially similar results were obtained using cloned Mac-1+ and Mac-1- MH-S cells. The finding of heterogeneity in an established cell line that displays functions similar to those of freshly recovered AMs suggests that distinct subsets of AMs may be involved in the pathogenesis of disease processes in the lung.

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Isolation of phenotypically and functionally distinct macrophage subpopulations from human bronchoalveolar lavage

Cited by 51 publications

References 37 publications

The phenotype of alveolar macrophages and its correlation with immune cells in bronchoalveolar lavage

The phenotype of alveolar macrophages and its correlation with immune cells in bronchoalveolar lavage

Morphological aspects of particle uptake by lung phagocytes

Phenotypic and functional heterogeneity of the murine alveolar macrophage-derived cell line MH-S

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