Isolation of phenazine 1,6-di-carboxylic acid from Pseudomonas aeruginosa strain HRW.1-S3 and its role in biofilm-mediated crude oil degradation and cytotoxicity against bacterial and cancer cells

Dasgupta, Debdeep; Kumar, Ashutosh; Mukhopadhyay, Balaram; Sengupta, Tapas K.

doi:10.1007/s00253-015-6707-x

Cited by 38 publications

(31 citation statements)

References 45 publications

(63 reference statements)

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“…The PDC phenazine was first isolated from Streptomyces species; it was demonstrated to be the potential agent for controlling metabolism and biofilm formation in Candida albicans [ 49 , 50 ]. Compared to other phenazine metabolites, PDC showed a substantially broader spectrum of cytotoxicity effect towards a number of cancer cells of different origins, including HT29, HeLa, and MCF7 cell lines, with less activity on DU145 (Human prostate cancer cell lines) [ 51 ].…”

Section: Bacteria As Anti-cancer Agents Through Released Substancesmentioning

confidence: 99%

The role of bacteria in cancer therapy – enemies in the past, but allies at present

Song

Vuai

Zhong

2018

Infect Agents Cancer

128

115

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In recent decades, bacteria’s therapeutic role has aroused attention in medicinal and pharmaceutical research. While bacteria are considered among the primary agents for causing cancer, recent research has shown intriguing results suggesting that bacteria can be effective agents for cancer treatment – they are the perfect vessels for targeted cancer therapy. Several bacterial strains/species have been discovered to possess inherent oncolytic potentials to invade and colonize solid tumors in vivo. The therapeutic strategy of using bacteria for treating cancer is considered to be effective; however, the severe side effects encountered during the treatment resulted in the abandonment of the therapy. State-of-the-art genetic engineering has been recently applied to bacteria therapy and resulted in a greater efficacy with minimum side effects. In addition, the anti-cancer potential of tumor-targeting bacteria through oral administration circumvents the use of the intravenous route and the associated adverse effects. This review aims to provide a comprehensive summary of the latest literature on the role of bacteria in cancer treatment.

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Section: Bacteria As Anti-cancer Agents Through Released Substancesmentioning

confidence: 99%

The role of bacteria in cancer therapy – enemies in the past, but allies at present

Song

Vuai

Zhong

2018

Infect Agents Cancer

128

115

View full text Add to dashboard Cite

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“…Indeed, PDC was mostly identified from Streptomyces species (Wang et al, 2011;Remali et al, 2017). PDC had not been widely discussed in the context of Pseudomonas until recently; researchers found that mutation of the phzA gene in Pseudomonas chlororaphis HT66 resulted in a significant increase in PDC production, which distinguishes the phenazine pathway producing only PCA from the pathway forming both PCA and PDC (Dasgupta et al, 2015;Guo et al, 2017; Figure 9). Another study reported the production of PDC in the genetically engineered strain Pseudomonas chlororaphis GP72ANlphzG (Guo et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomic and Metabolomic Analyses of Two Pseudomonas aeruginosa Strains With Different Antifungal Activities

et al. 2020

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Pseudomonas aeruginosa isolated from the plant rhizosphere has been widely used as an effective strain in biological control against plant disease. This bacterium promotes plant growth and protect plants against various phytopathogens through the production of phenazine metabolites. In this study, the strain P. aeruginosa Y12 with anti-Beauveria bassiana activity was isolated from the gut of housefly larvae. It was comparatively analyzed with the strain P. aeruginosa P18, which showed no anti-B. bassiana activity. Genomic and metabolomic methods were used to obtain a comprehensive understanding of the antimicrobial mechanism of Y12. After whole-genome resequencing of the two strains, a total of 7,087 non-synonymous single-nucleotide polymorphisms (nsSNPs), 1079 insertions and deletions (InDels), 62 copy-number variations (CNVs) and 42 structural variations (SV) were found in both strains. We analyzed the differentially abundant metabolites between Y12 and P18, and identified six bioactive compounds that could be associated with the antimicrobial activity of Y12. Additionally, we found that, unlike other previously reported rhizospheric P. aeruginosa strains, Y12 could produce both phenazine-1,6-dicarboxylic acid (PDC) and pyocyanin (PYO) at significantly higher concentrations than P18. As B. bassiana is an effective biological insecticide that can cause high mortality in adult houseflies but has little effect on housefly larvae, we believe that P. aeruginosa Y12, identified in housefly larvae but not in adults, were beneficial for the development of housefly larvae and could protect them from B. bassiana infection through the production of toxic metabolites.

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“…The phenazines are small molecules that can penetrate different types of cells, including prokaryotes. Many phenazines present antimicrobial activity, suppressing pathogenic microorganisms of humans, animals and plants, also playing an important role in the survival strategies of bacteria in the environment (Chen et al, 2014 ; Briard et al, 2015 ; Dasgupta et al, 2015 ; Garrison et al, 2016 ; Morrison et al, 2016 ). The P. aeruginosa LV strain is a great producer of phenazine carboxylic acid (PCA).…”

Section: Discussionmentioning

confidence: 99%

New Insights about Antibiotic Production by Pseudomonas aeruginosa: A Gene Expression Analysis

et al. 2017

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The bacterial resistance for antibiotics is one of the most important problems in public health and only a small number of new products are in development. Antagonistic microorganisms from soil are a promising source of new candidate molecules. Products of secondary metabolism confer adaptive advantages for their producer, in the competition for nutrients in the microbial community. The biosynthesis process of compounds with antibiotic activity is the key to optimize their production and the transcriptomic study of microorganisms is of great benefit for the discovery of these metabolic pathways. Pseudomonas aeruginosa LV strain growing in the presence of copper chloride produces a bioactive organometallic compound, which has a potent antimicrobial activity against various microorganisms. The objective of this study was to verify overexpressed genes and evaluate their relation to the organometallic biosynthesis in this microorganism. P. aeruginosa LV strain was cultured in presence and absence of copper chloride. Two methods were used for transcriptomic analysis, genome reference-guided assembly and de novo assembly. The genome referenced analysis identified nine upregulated genes when bacteria were exposed to copper chloride, while the De Novo Assembly identified 12 upregulated genes. Nineteen genes can be related to an increased microbial metabolism for the extrusion process of exceeding intracellular copper. Two important genes are related to the biosynthesis of phenazine and tetrapyrroles compounds, which can be involved in the bioremediation of intracellular copper and we suggesting that may involve in the biosynthesis of the organometallic compound. Additional studies are being carried out to further prove the function of the described genes and relate them to the biosynthetic pathway of the organometallic compound.

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Isolation of phenazine 1,6-di-carboxylic acid from Pseudomonas aeruginosa strain HRW.1-S3 and its role in biofilm-mediated crude oil degradation and cytotoxicity against bacterial and cancer cells

Cited by 38 publications

References 45 publications

The role of bacteria in cancer therapy – enemies in the past, but allies at present

The role of bacteria in cancer therapy – enemies in the past, but allies at present

Comparative Genomic and Metabolomic Analyses of Two Pseudomonas aeruginosa Strains With Different Antifungal Activities

New Insights about Antibiotic Production by Pseudomonas aeruginosa: A Gene Expression Analysis

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