Isolation of phenanthrene-degrading bacteria and characterization of phenanthrene metabolites

Abstract

Three aerobic bacterial consortia GY2, GS3 and GM2 were enriched from polycyclic aromatic hydrocarbon-contaminated soils with water-silicone oil biphasic systems. An aerobic bacterial strain utilizing phenanthrene as the sole carbon and energy source was isolated from bacterial consortium GY2 and identified as Sphingomonas sp. strain GY2B. Within 48 h and at 30°C the strain metabolized 99.1% of phenanthrene (100 mg/l) added to batch culture in mineral salts medium and the cell number increased by about 40-fold. Three metabolites 1-hydroxy-2-naphthoic acid, 1-naphthol and salicylic acid, were identified by gas chromatographic mass spectrometry and UV-visible spectroscopy analysis. A degradation pathway was proposed based on the identified metabolites. In addition to phenanthrene, strain GY2B could use other aromatic compounds such as naphthalene, 2-naphthol, salicylic acid, catechol, phenol, benzene and toluene as a sole source of carbon and energy.

Characterisation of a thermostable catechol-2,3-dioxygenase from phenanthrene-degradingPseudomonas sp. strain ZJF08

“…There were no fluctuations in both enzymes activities during 7 days and the activities were much lower than those in MM with phenanthrene (0.03 U and 0.07 U of PDO and C23O respectively). The observation suggested that the two key enzymes might be inducible enzymes and it was consistent with the results from other reports (Tian et al, 2002;Tao et al, 2007).…”

Using a Bayesian approach to improve and calibrate a dynamic model of polycyclic aromatic hydrocarbons degradation in an industrial contaminated soil

“…In contrast, kinetic values obtained with scenario 2 approach those found in the literature and increase thereby the confidence in the model predictions. Indeed, the model predicts that the specific biomass doubles within 5 h, which is in accordance with the values published by Tao et al (2007) for a microbial biomass using the phenanthrene as the sole source of carbon and energy in PAHs contaminated soil. The most likely biomass yield, Y spe, is equal to 0.127, which is about half of the value found by Weissenfels et al (1990) for phenanthrene within a pure bacterial culture, but was close to the value of 0.158 proposed by K€ astner et al (2013) for the anthracene (knowing that anthracene is 3-cycles PAHs close to phenanthrene).…”

“…They were chosen based on available literature studies (DelgadilloMirquez et al, 2011;Fragoulis et al, 2005;Geng et al, 2015;Johnson et al, 2001;K€ ohler et al, 1994;Tao et al, 2007;Weissenfels et al, 1990;Wick et al, 2001) and further adjusted using a stepwise trial and error strategy. The model was run with the initial conditions observed a few minutes after the beginning of the experiment: PAH CO2 , PAH Bs , PAH SS and PAH MET were null; all 13 C-PHE in soil was PAH WS and PAH AV as described in section 2.2.…”

Flocculant in wastewater affects dynamics of inorganic N and accelerates removal of phenanthrene and anthracene in soil

“…The high pH and large EC may have inhibited removal of PAH from the Texcoco soil, but did not stop it. Polycyclic aromatic hydrocarbons can be degraded by microorganisms able to grow in the presence of salt (Tao et al, 2007) and at pH > 10 ( Gerbeth et al, 2004). These data suggest the flocculant accelerated the removal of phenanthrene and anthracene from the Acolman and Texcoco soil.…”