Isolation of peste des petits ruminants virus using primary goat kidney cell culture from kidneys obtained at slaughter

Begum, Shamim Ara; Nooruzzaman, Mohammed; Hasnat, Azmary; Parvin, Mst. Murshida; Parvin, Rokshana; Islam, Mohammad Rafiqul; Chowdhury, Emdadul Haque

doi:10.1002/vms3.413

Cited by 3 publications

(2 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PPRV strain (BD/PPRV/2015/1) was originally collected from a field outbreak in Black Bengal goats in 2015 (10) and subsequently isolated in primary goat kidney cells. The PPRV inoculum was prepared using primary goat kidney cell culture (25) and the presence of virus in the culture supernatant was tested by realtime quantitative RT-PCR (qPCR) as described previously (26). Finally, the amount of virus in the culture supernatant was titrated using 96 well flat bottom tissue culture plates (27) and the end-point titer was calculated by Reed and Muench method (28).…”

Section: Virus and Cell Culturementioning

confidence: 99%

A Sequential Study on the Pathology of Peste Des Petits Ruminants and Tissue Distribution of the Virus Following Experimental Infection of Black Bengal Goats

et al. 2021

Self Cite

View full text Add to dashboard Cite

We studied the sequential pathology of peste des petits ruminants (PPR) in Black Bengal goats and analyzed virus distribution in tissues and virus shedding following experimental infection with a Bangladeshi isolate of lineage IV PPR virus (PPRV). The early clinical signs like fever, depression, and ocular and nasal discharges first appeared at 4–7 days post-infection (dpi). Three out of eight inoculated goats died at 13, 15, and 18 dpi, and the rest were killed at different time points from 5 to 18 dpi. Initially, the virus multiplied mostly in the lymphoid organs of the pharyngeal region and caused extensive lymphoid destruction and hemorrhages. This was followed by viremia, massive virus replication in the lungs, and pneumonia along with the appearance of the clinical signs. Subsequently, the virus spread to other organs causing necrotic and hemorrhagic lesions, as well as the virus localized in the upper respiratory, oral and intestinal mucosa resulting in catarrhal, erosive, and ulcerative lesions. On hematological and biochemical investigation progressive leukopenia and hypoproteinemia, a gradual increase of serum metabolites and enzymes associated with liver and kidney damage, and electrolyte imbalance were observed. Seroconversion started at 7 dpi and all the surviving animals had serum antibodies at 14 dpi. Virus shedding was observed in nasal and ocular secretions at 4 dpi and in feces and urine at 14 dpi, which gradually increased and continued till the end of the experiment (18 dpi) despite seroconversion. Therefore, the virus shedding of naturally infected seroconverted goats should be monitored for effective control strategies.

show abstract

Section: Virus and Cell Culturementioning

confidence: 99%

A Sequential Study on the Pathology of Peste Des Petits Ruminants and Tissue Distribution of the Virus Following Experimental Infection of Black Bengal Goats

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Currently, Vero cells or some cell lines expressing PPRV receptors are mainly used for PPRV studies in vitro , but little is known about PPRV in goat cells ( Kumar et al., 2013 ; Clarke et al., 2018 ; Begum et al., 2021 ). Because the natural hosts of PPRV are mainly small ruminants such as goats and sheep, goat cells should be suitable for the study of viral infection and immunity to PPRV, and only such evidence can objectively reflect the pathogenic and natural immune mechanisms of PPRV in the host.…”

Section: Discussionmentioning

confidence: 99%

Peste des Petits Ruminants Virus Exhibits Cell-Dependent Interferon Active Response

Tang

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Peste des petits ruminants (PPR) is an acute and highly pathogenic infectious disease caused by peste des petits ruminants virus (PPRV), which can infect goats and sheep and poses a major threat to the small ruminants industry. The innate immune response plays an important role as a line of defense against the virus. The effect of PPRV on the active innate immune response has been described in several studies, with different conclusions. We infected three goat-derived cell lines with PPRV and tested their innate immune response. PPRV proliferated in caprine endometrial epithelial cells (EECs), caprine skin fibroblasts cells (GSFs), and goat fibroblast cells (GFs), and all cells expressed interferon (IFN) by poly (I: C) stimulation. PPRV infection stimulated expression of type I and type III IFN on EECs, and expression of the latter was significantly stronger, but IFN was not stimulated in fibroblasts (GSFs and GFs). Our results suggested that the effect of PPRV on IFN was cell-type specific. Nine IFN-stimulated genes (ISGs) were detected in EECs, but only ISG15 and RSAD2 were significantly upregulated. The effects of PPRV on IFN and IFN-induced ISGs were cell-type specific, which advances our understanding of the innate immune response induced by PPRV and creates new possibilities for the control of PPRV infection.

show abstract

A Simple and Versatile Method for Ex Vivo Monitoring of Goat Vaginal Mucosa Transduction by Viral Vector Vaccines

Minesso,

Odigie,

Franceschi

et al. 2024

Vaccines

View full text Add to dashboard Cite

Goat may represent a valid large animal model for human pathogens and new vaccines testing. Appropriate vaccine administration is a critical component of a successful immunization program. The wrong route of administration may reduce the efficacy of the vaccine, whereas the proper administration strategy can enhance it. Viral vectors have been employed successfully for goat and sheep immunization; however, no data concerning the vaginal route are available. A viral vector’s ability to transduce the site of inoculation is of primary interest. In this study, a fast and reliable ex vivo assay for testing the transduction capability of an Ad5-based vector when intravaginally administered was developed. An Ad5 vector delivering an expression cassette with a bicistronic reporter gene, Ad5-CMV-turboGFP-IRES-Luc2, was constructed. We demonstrated Ad5-CMV-turboGFP-IRES-Luc2’s ability to transduce caprine vaginal mucosa by ex vivo bioluminescent imaging (BLI) employing a simple CCD camera apparatus for chemiluminescence western immunoblotting. These data, though simple, provide valuable insights into developing a vaginal immunization strategy using a viral vector-based vaccine to protect against pathogens causing genital diseases.

show abstract

Isolation of peste des petits ruminants virus using primary goat kidney cell culture from kidneys obtained at slaughter

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 3 publications

References 24 publications

A Sequential Study on the Pathology of Peste Des Petits Ruminants and Tissue Distribution of the Virus Following Experimental Infection of Black Bengal Goats

A Sequential Study on the Pathology of Peste Des Petits Ruminants and Tissue Distribution of the Virus Following Experimental Infection of Black Bengal Goats

Peste des Petits Ruminants Virus Exhibits Cell-Dependent Interferon Active Response

A Simple and Versatile Method for Ex Vivo Monitoring of Goat Vaginal Mucosa Transduction by Viral Vector Vaccines

Contact Info

Product

Resources

About