Isolation of pathogenic microorganisms from burn patient and in vitro determination of antibacterial activity of honey against antibiotic resistance isolates

Abstract

<p class="abstract"><strong>Background:</strong> Honey is a natural therapeutic agent which manifest antimicrobial activity against a wide range of bacteria. Therefore, the current study was designed to isolate pathogenic bacteria from burn wound and also to determine the anti-bacterial traits of natural and processed honey against infectious agents.</p><p class="abstract"><strong>Methods:</strong> Wound samples were collected from burn unit of Dhaka Medical College Hospital and conventional cultural methods were applied to identify pathogenic microorganisms. A total of six samples including three each of natural and processed honey were tested for the determination of antimicrobial activity by agar well diffusion method. </p><p class="abstract"><strong>Results:</strong> Among ten wound samples highest load of total viable bacteria was recorded up to 3.7×10<sup>6</sup> cfu/ml. The maximum load of <em>Pseudomonas </em>spp. and <em>Staphylococcus </em>spp. were found up to 1.6×10<sup>4</sup> cfu/ml and 8.7×10<sup>4</sup> cfu/ml respectively. Significant <em>in vitro</em> antimicrobial activity was found in all the samples. Natural honey showed a little bit more efficacy than processed honey. The samples exhibited antibacterial traits against <em>Staphylococcus aureus</em> with a wide zone of inhibition and moderate zone of inhibition against <em>Pseudomonas </em>spp. when they are subjected to 100% concentered honey. <em>E. coli</em> and <em>Klebsiella </em>spp. were remained to be unaffected at 75% and 50% concentrated honey, while <em>S. aureus</em> and <em>Pseudomonas </em>spp. were found to be sensitive at those concentrations.</p><p class="abstract"><strong>Conclusions:</strong> The <em>in vitro</em> efficacy of different types of honey tested against the bacteria dependent on the type of honey and the concentration at which it was administered. In our study 100% concentred honey was more efficient in inhibiting all the tested isolates.</p>