Isolation of osteogenic growth peptide from osteoblastic MC3T3 E1 cell cultures and demonstration of osteogenic growth peptide binding proteins

Greenberg, Zvi; Gavish, Hanna; Mühlrád, András; Chorev, Michael; Shteyer, A.; Attar-Namdar, Malka; Tartakovsky, Alexander G.; Bab, Itai

doi:10.1002/(sici)1097-4644(19970601)65:3<359::aid-jcb6>3.0.co;2-r

Cited by 33 publications

(35 citation statements)

References 23 publications

(48 reference statements)

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“…Cells were cultured in serum-free conditions to measure the direct action of OGP analogs on hMSCs proliferation without masking effects from serum-OGP. As soluble peptides, the classical forms of OGP and OGP10-14 have been shown to regulate the proliferation of osteoblastic and stromal cells in a biphasic-dependent manner, indicative of an autocrine/paracrine mode of action [7,14]. As depicted in Fig.…”

Section: Effect Of Free and Alginate-conjugated Ogp Analogs In Hmscs:mentioning

confidence: 91%

“…After 1 week of culture in BM, ALP activity was increased in the presence of OGP, except when OGP2 was used at 10 −5 M. In OM, ALP activity increased dramatically in the presence of OGP2, especially when present at 10 −12 M, a concentration that has been defined as being within the optimal effective range in previous studies, and which typically lies between 10 −13 M and 10 −8 M [7,14,39].…”

Section: Effect Of Free and Alginate-conjugated Ogp Analogs In Hmscs:mentioning

confidence: 99%

“…Up to now, different types of osteoinductive peptides have been proposed [4]. In this study, we evaluated the potential of OGP, a naturally occurring tetradeca-peptide identical to the C-terminus of histone H4 (residues 89-102, ALKRQGRTLYGFGG), which is present in plasma at micromolar concentrations [5][6][7][8]. The physiologically active form of OGP, which corresponds to its C-terminal pentapeptide sequence YGFGG (OGP10-14), is generated from full-length OGP by proteolytic cleavage [5].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells

Maia

Barbosa²,

Gomes

et al. 2014

Journal of Controlled Release

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The outcome of cell-based therapies can benefit from carefully designed cell carriers. A multifunctional injectable vehicle for the co-delivery of human mesenchymal stem cells (hMSCs) and osteoinductive peptides is proposed, to specifically direct hMSCs osteogenic differentiation. The osteogenic growth peptide (OGP) inspired the design of two peptides, where the bioactive portion of OGP was flanked by a protease-sensitive linker, or its scrambled sequence, to provide faster and slower release rates, respectively. Peptides were fully characterized and chemically grafted to alginate. Both OGP analogs released bioactive fragments in vitro, at different kinetics, which stimulated hMSCs proliferation and osteogenesis. hMSCs-laden OGP-alginate hydrogels were tested at an ectopic site in a xenograft mouse model. After 4 weeks, OGP-alginate hydrogels were more degraded and colonized by vascularized connective tissue than the control (without OGP). hMSCs were able to proliferate, migrate outward the hydrogels, produce endogenous extracellular matrix and mineralize it. Moreover, OGP-groups stimulated hMSCs osteogenesis, as compared with the control. Overall, the ability of the proposed platform to direct the fate of transplanted hMSCs in loco was demonstrated, and OGP-releasing hydrogels emerged as a potentially useful system to promote bone regeneration.

show abstract

Section: Effect Of Free and Alginate-conjugated Ogp Analogs In Hmscs:mentioning

confidence: 91%

Section: Effect Of Free and Alginate-conjugated Ogp Analogs In Hmscs:mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells

Maia

Barbosa²,

Gomes

et al. 2014

Journal of Controlled Release

View full text Add to dashboard Cite

show abstract

“…OGP increases bone formation and trabecular bone density and regulates proliferation and osteogenic maturation of MSC (Robinson et al, 1995;Greenberg et al, 1997). It has been shown to be a mitogen for MSC and, furthermore, the simultaneous treatment of OGP and basic fibroblast growth factor (bFGF) results in a three-fold stimulatory effect as compared to OGP alone on MSC (Guido et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture

Zhu¹,

Guo²,

Yang³

et al. 2008

Vet. Med. - Czech

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S:The osteogenic growth peptide (OGP) regulates the differentiation of marrow mesenchymal stem cells derived from human and rodent cell lines into osteoblasts. Whether OGP directly regulates the bovine marrow mesenchymal stem cells differentiating into osteoblasts remains unknown. In this study, we evaluated the effects of OGP on the growth and differentiation of bovine marrow mesenchymal stem cells in culture. Our results showed that OGP promoted osteogenic differentiation of the bovine stem cells. OGP increased alkaline phosphatase (ALP) activity and mineralized nodule formation, and stimulated osteoblast-specific mRNA expression of Osteocalcin (BGP). On the other hand, OGP dose-dependently stimulated the expression of endothelial nitric oxide synthases. These results show for the first time a direct osteogenic effect of OGP on bovine marrow stromal cells in culture, which could be mediated by induction of endothelial nitric oxide synthases.

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“…16 Furthermore, some self-assembling peptides, directly incorporating various functional motifs into RADA16-I, are designed to mimic particular ECM. These motifs include collagen (GGDGEA), 17 laminin (GGPDSGR), 18 fibronectin, 19 osteopontin and osteogenic peptides, 20 and myelo-regulatory peptides. 21 These functionalized peptides also self-assemble into three-dimensional nanofiber scaffolds.…”

Section: Introductionmentioning

confidence: 99%

A self-assembling peptide RADA16-I integrated with spider fibroin uncrystalline motifs

Sun¹,

Wang²

2012

IJN

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Mechanical strength of nanofiber scaffolds formed by the self-assembling peptide RADA16-I or its derivatives is not very good and limits their application. To address this problem, we inserted spidroin uncrystalline motifs, which confer incomparable elasticity and hydrophobicity to spider silk GGAGGS or GPGGY, into the C-terminus of RADA16-I to newly design two peptides: R3 (n-RADARADARADARADA-GGAGGS-c) and R4 (n-RADARADARADARADA-GPGGY-c), and then observed the effect of these motifs on biophysical properties of the peptide. Atomic force microscopy, transmitting electron microscopy, and circular dichroism spectroscopy confirm that R3 and R4 display β-sheet structure and self-assemble into long nanofibers. Compared with R3, the β-sheet structure and nanofibers formed by R4 are more stable; they change to random coil and unordered aggregation at higher temperature. Rheology measurements indicate that novel peptides form hydrogel when induced by DMEM, and the storage modulus of R3 and R4 hydrogel is 0.5 times and 3 times higher than that of RADA16-I, respectively. Furthermore, R4 hydrogel remarkably promotes growth of liver cell L02 and liver cancer cell SMCC7721 compared with 2D culture, determined by MTT assay. Novel peptides still have potential as hydrophobic drug carriers; they can stabilize pyrene microcrystals in aqueous solution and deliver this into a lipophilic environment, identified by fluorescence emission spectra. Altogether, the spider fibroin motif GPGGY most effectively enhances mechanical strength and hydrophobicity of the peptide. This study provides a new method in the design of nanobiomaterials and helps us to understand the role of the amino acid sequence in nanofiber formation.

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Isolation of osteogenic growth peptide from osteoblastic MC3T3 E1 cell cultures and demonstration of osteogenic growth peptide binding proteins

Cited by 33 publications

References 23 publications

Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells

Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells

Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture

A self-assembling peptide RADA16-I integrated with spider fibroin uncrystalline motifs

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