Isolation of Oncornaviruses from Continuous Human Cell Cultures

Abstract

Sixteen cultures of continuous human cell lines were tested for oncornaviruses. Six cultures spontaneously released theviruses into the culture fluid. Five cultures released the virusesafter treatment with 5-bromodeoxyuridine or mitomycin C. Five cultures were negative. The viruses belonged to the B and Ctypes of oncornaviruses. Possible origin of the viruses is discussed.

Detection of oncornavirus‐like particles in HeLa cells. I. Fine structure and comparative morphological classification

“…Since completion of this work, Russian authors have published several papers o n the characterization of '' oncornaviruses from continuous human cell cultures" (Zhdanov et al, 1972;Zhdanov et al, 1973). Most of the particles observed in HeLa and other cells were grouped as B-type, and only some as C-type particles.…”

Common Antigens in Oncornaviruses from Continuous Human Cell Lines and Mason-Pfizer Virus

“…It is seen from this table that both MPMV and HEp2sera react with cellular and extracellular antigens from 7 of 11 cultures, described in the previous paper [2], as well as with MPMV antigens. Positive reactions were obtained with both cellular and extracellular (viral) antigens.…”

“…The data presented show that 7 human continuous cell cultures producing the B-type oncornaviruses [2] contain both extracellular and intracellular antigen closely related to antigen of MPMV. This conclusion is supported by immunodiffusion tests with MPMV and HEp2 sera.…”

“…Extracellular viruses from HEp2, HcLa, DAPT, RH, Detroit 6, AO and AI cells were isolated and purified as described earlier [2], Immune sera against MMTV. MPMV and HEp2 virus were obtained by immunization of rabbits in the popliteal lymph nodes with concentrated, purified virus preparations mixed with the complete Freund's adjuvant and were freed from tissue-specific and heterologous nonviral antibodies [1,5].…”

“…Oncornaviruses have been isolated from continuous human cell lines [1,2], which have morphologic similarities with the mouse mammary tumor virus (MMTV) [3] and the Mason-Pfizer monkey virus (MPMV) [4], This paper presents the results of the comparative study of antigens of these viruses.…”