Isolation of O1 Serovars of Vibrio cholerae from Water by Serologically Specific Method

Abstract

Vibrio cholerae bacteria of the serological variety O1 were consistently isolated from water samples by passing the water with added Tween 20 through columns packed with polystyrene beads coated with antibodies against the O1 antigenic determinants. The beads from the columns were washed, transferred to alkaline peptone broth, and incubated. The O1 serovars were isolated and identified by established procedures.

Detection of Vibrio cholerae with monoclonal antibodies specific for serovar O1 lipopolysaccharide

“…We envision the direct fluorescence assay, in which an O1-specific MAb-FITC conjugate is used, could be used to address the question of whether the V. cholerae El Tor Inaba, which emerges each August and September in Louisiana, is present in environmental samples throughout the year, even when it cannot be retrieved by bacteriological culture. Anti-A antigen MAb immobilized on latex beads could be used to capture the vibrio from water or homogenized seafood preparations percolated through the column (27) to facilitate culturing of the 01 vibrios. Finally, the MAb-FITC reagent permits reinvestigation into the utility of this reagent to detect cholera vibrios in direct fecal smears first pursued by Finkelstein and LeBrec (17) and abandoned by Thomason et al (50) because polyclonal 01 sera produced nonspecific staining.…”

New approach in detecting phytopathogenic bacteria by combined immunoisolation and immunoidentification assays1

“…Immunosorbent enrichment is possible in polystyrene test tubes or wells of microplates (as for ELISA), filled after the immunoselecting steps with semi-selective or elective broth . Alternatively, beads with immunotrapped bacteria can be incubated in enrichment broth (Hranitzky et al, 1980). This selective enrichment procedure can be used for: (a) growth of target bacteria before use in other test systems (ELISA or test-plant inoculation); (b) isolation of the target bacterium or cross-reacting bacteria on various media; (c) quantification of target bacteria in a sample extract by statistical methods such as the most probable number method (Swaroop,195 1).…”