Isolation of novel leaf-inhabiting endophytic bacteria in Arabidopsis thaliana and their antagonistic effects on phytophathogens

Draft Genome Sequence of the Endophytic Bacterium Variovorax paradoxus KB5, Which Has Antagonistic Activity against a Phytopathogen, Pseudomonas syringae pv. tomato DC3000

“…Variovorax paradoxus is a plant growth-promoting endophytic bacterium known for its metabolic versatility, including its degradation of diverse environmental contaminants ( 1 ). Previously, we isolated bacteria that have antimicrobial activity against plant bacterial pathogens while they are present in leaves of Arabidopsis thaliana plants ( 2 ). One of these bacteria, strain KB5, was identified as V. paradoxus based on 16S rRNA sequence similarity ( 2 ).…”

“…Previously, we isolated bacteria that have antimicrobial activity against plant bacterial pathogens while they are present in leaves of Arabidopsis thaliana plants ( 2 ). One of these bacteria, strain KB5, was identified as V. paradoxus based on 16S rRNA sequence similarity ( 2 ).…”

Genome Sequence of the Endophytic Bacterium Bacillus thuringiensis Strain KB1, a Potential Biocontrol Agent against Phytopathogens

“…It had been shown that the endophytic strain KB1, originally isolated from the apoplastic fluid extracts of Arabidopsis thaliana , significantly increases the disease resistance of tomatoes to Botrytis cinerea and Pseudomonas syringae pv. tomato ( 6 ).…”

“…KB1 was formerly classified into B. cereus based on a 16S rRNA gene sequence analysis ( 6 ). However, multilocus sequence analysis using the PubMLST resource ( http://pubmlst.org/bcereus/ ) ( 10 ), which utilizes eight housekeeping genes for the typing of the Bacillus cereus group, revealed only one nucleotide difference at the pyc gene out of eight loci between KB1 and B. thuringiensis serovar tochigiensis HD868 (= BGSC 4Y1, GenBank CM000746.1) ( 11 , 12 ).…”

Draft Genome Sequence of the Endophytic Strain Rhodococcus kyotonensis KB10, a Potential Biodegrading and Antibacterial Bacterium Isolated from Arabidopsis thaliana

“…Rhodococcus species show antagonistic activity against human pathogens and the phytopathogens Ceratocystis fimbriata and Pseudomonas syringae pv. tomato DC3000 ( 1 , 4 , 5 ). Therefore, Rhodococcus may be an attractive genus for the bioremediation of environmental pollutants and for biocontrol in an agricultural setting ( 6 ).…”