Isolation of Novel CO Converting Microorganism Using Zero Valent Iron for a Bioelectrochemical System (BES)

Im, Hyeon Sung; Kim, Changman; Song, Young Eun; Baek, Jiyun; Im, Chae Ho; Kim, Eun Jung

doi:10.1007/s12257-018-0373-7

Cited by 24 publications

(7 citation statements)

References 30 publications

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“…Other bacteria from the genus Petrimonas, known as fermentative bacteria, which can use carbohydrates and some organic acids [70] and Alistipe, which produce volatile fatty acids (VFAs), including succinate, acetate and propionate, were also identified [71]. Recently, Im and collaborators used zero valent iron as electron donor to isolate CO converting microorganisms [72]. The authors performed sequential batch assays using zero valent iron as electron donor under CO as headspace gas.…”

Section: Microbiology Of Co/syngas Conversionmentioning

confidence: 99%

Bioelectrochemical systems (BESs) towards conversion of carbon monoxide/syngas: A mini-review

Barbosa

Peixoto

Alves

et al. 2021

Renewable and Sustainable Energy Reviews

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Microbial conversion of carbon monoxide (CO)/syngas has been extensively investigated. The microbial conversion of CO/syngas offers numerous advantages over chemically catalyzed processes e.g. the specificity of the biocatalysts, the operation at ambient conditions and high conversion efficiencies. Bioelectrochemical systems (BESs) exploit the capacity of electrochemically active bacteria (EAB) to use insoluble electron acceptors or donors to produce electricity or added-value compounds. Electricity production from different organic sources in BESs has been broadly demonstrated, whereas electricity production from CO/syngas has been very little reported. Acetate oxidation by a consortium of carboxydotrophic and CO-tolerant EAB has been suggested to be the main pathway responsible for indirect electricity generation from CO/syngas. Although electricity production in BESs from several organic sources has been widely investigated, the interest on BESs research is currently moving to the production of added-value compounds by electro-fermentation (EF) processes. EF allows to modify redox balances by the use of electric circuits to fine tune metabolic pathways towards obtaining products with high economic value. Although EF has been widely studied, the potential of use CO-rich gas streams as substrate has been under explored. This review presents and discusses current advances on microbial conversion of CO/syngas in BESs.

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Section: Microbiology Of Co/syngas Conversionmentioning

confidence: 99%

Bioelectrochemical systems (BESs) towards conversion of carbon monoxide/syngas: A mini-review

Barbosa

Peixoto

Alves

et al. 2021

Renewable and Sustainable Energy Reviews

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“…The concentrations of acetate, butyrate, and glucose were determined using a high-performance liquid chromatography (HPLC) system (Prostar; Varian, Palo Alto, CA, United States) equipped with a packed column (Metacarb 87H; MetaChem Technologies, Torrance, CA, United States) and refractive index detector (RI-27; Shodex, Japan). The mobile phase consisted of 0.01 N H 2 SO 4 ( Im et al, 2019 ; Chun and Sang, 2020 ; Lee et al, 2020 ). The concentrations of acetone, butanol, and ethanol were determined using a gas chromatography system (Agilent 7890; Agilent Technologies, California, United States) equipped with a packed column (80/120 Carbopack BAW glass column; Supelco, Bellefonte, PA, United States) and flame ionization detector ( Jang et al, 2012 ; Baek et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures

Jang

Seong

Kwon

et al. 2021

Front. Bioeng. Biotechnol.

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ATPase, a key enzyme involved in energy metabolism, has not yet been well studied in Clostridium acetobutylicum. Here, we knocked down the atpG gene encoding the ATPase gamma subunit in C. acetobutylicum ATCC 824 using a mobile group II intron system and analyzed the physiological characteristics of the atpG gene knockdown mutant, 824-2866KD. Properties investigated included cell growth, glucose consumption, production of major metabolites, and extracellular pH. Interestingly, in 2-L batch fermentations, 824-2866KD showed no significant difference in metabolite biosynthesis or cell growth compared with the parent ATCC 824. However, the pH value in 824-2866KD cultures at the late stage of the solventogenic phase was abnormally high (pH 6.12), compared with that obtained routinely in the culture of ATCC 824 (pH 5.74). This phenomenon was also observed in batch cultures of another C. acetobutylicum, BEKW-2866KD, an atpG-knockdown and pta-buk double-knockout mutant. The findings reported in this study suggested that ATPase is relatively minor than acid-forming pathway in ATP metabolism in C. acetobutylicum.

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“…Several studies reported that electron transfer from electrode to bacteria induced the metabolic shifts of K. pneumoniae L17 in a bioelectrochemical system (BES) and increased 1,3-PDO production [13,21,22]. Similarly, zero-valent iron (ZVI) was also demonstrated as the feasible electron source for a K. pneumoniae strain for enhancement of 1,3-PDO production [23,24].…”

Section: Crude Glycerolmentioning

confidence: 99%

Bioconversion of Crude Glycerol into 1,3-Propanediol(1,3-PDO) with Bioelectrochemical System and Zero-Valent Iron Using Klebsiella pneumoniae L17

et al. 2021

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Crude glycerol is a major byproduct in the production of biodiesel and contains a large number of impurities. The transformation of crude glycerol into valuable compounds such as 1,3-propanediol (1,3-PDO) using clean and renewable processes, like bioconversion, is an important task for the future of the chemical industry. In this study, 1,3-PDO bioproductions from crude and pure glycerol were estimated as 15.4 ± 0.8 and 11.4 ± 0.1 mmol/L, respectively. Because 1,3-PDO is a reductive metabolite that requires additional reducing energy, external supplements of electron for further improvement of 1,3-PDO biosynthesis were attempted using a bioelectrochemical system (BES) or zero-valent iron (ZVI). The conversions of crude and pure glycerol under electrode and iron-based cultivation were investigated for 1,3-PDO production accompanied by metabolic shift and cell growth. The BES-based conversion produced 32.6 ± 0.6 mmol/L of 1,3-PDO with ZVI implementation.

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Isolation of Novel CO Converting Microorganism Using Zero Valent Iron for a Bioelectrochemical System (BES)

Cited by 24 publications

References 30 publications

Bioelectrochemical systems (BESs) towards conversion of carbon monoxide/syngas: A mini-review

Bioelectrochemical systems (BESs) towards conversion of carbon monoxide/syngas: A mini-review

Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures

Bioconversion of Crude Glycerol into 1,3-Propanediol(1,3-PDO) with Bioelectrochemical System and Zero-Valent Iron Using Klebsiella pneumoniae L17

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