Isolation of nisin-producing Lactococcus lactis WNC 20 strain from nham, a traditional Thai fermented sausage

Noonpakdee, Wilai; Santivarangkna, Chalat; Jumriangrit, P.; Sonomoto, Kenji; Panyim, Sakol

doi:10.1016/s0168-1605(02)00219-2

Cited by 144 publications

(84 citation statements)

References 42 publications

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“…The primers were designed from nisin and pediocin PA-1/AcH structural genes. The nisin gene primers were complementary to regions 17 bp upstream (5Ј CCGGAATTCATAAGGAGGC ACTCAAAATG 3Ј) and 2 bp downstream (3Ј CGGGGTACCTACTATCCTT TGATTTGGTT 5Ј) of the coding region for nisin (24), and the pediocin gene primers were as described by Millette et al (18). The EcoRI and KpnI restriction sites were added at the 5Ј ends of primer 1 and primer 2, respectively, for cloning purposes.…”

Section: Methodsmentioning

confidence: 99%

Capacity of Human Nisin- and Pediocin-Producing Lactic Acid Bacteria To Reduce Intestinal Colonization by Vancomycin-Resistant Enterococci

Millette

Cornut

Dupont

et al. 2008

Appl Environ Microbiol

138

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This study demonstrated the capacity of bacteriocin-producing lactic acid bacteria (LAB) to reduce intestinal colonization by vancomycin-resistant enterococci (VRE) in a mouse model. Lactococcus lactis MM19 and Pediococcus acidilactici MM33 are bacteriocin producers isolated from human feces. The bacteriocin secreted by P. acidilactici is identical to pediocin PA-1/AcH, while PCR analysis demonstrated that L. lactis harbors the nisin Z gene. LAB were acid and bile tolerant when assayed under simulated gastrointestinal conditions. A well diffusion assay using supernatants from LAB demonstrated strong activity against a clinical isolate of VRE. A first in vivo study was done using C57BL/6 mice that received daily intragastric doses of L. lactis MM19, P. acidilactici MM33, P. acidilactici MM33A (a pediocin mutant that had lost its ability to produce pediocin), or phosphate-buffered saline (PBS) for 18 days. This study showed that L. lactis and P. acidilactici MM33A increased the concentrations of total LAB and anaerobes while P. acidilactici MM33 decreased the Enterobacteriaceae populations. A second in vivo study was done using VRE-colonized mice that received the same inocula as those in the previous study for 16 days. In L. lactis-fed mice, fecal VRE levels 1.73 and 2.50 log 10 CFU/g lower than those in the PBS group were observed at 1 and 3 days postinfection. In the P. acidilactici MM33-fed mice, no reduction was observed at 1 day postinfection but a reduction of 1.85 log 10 CFU/g was measured at 3 days postinfection. Levels of VRE in both groups of mice treated with bacteriocin-producing LAB were undetectable at 6 days postinfection. No significant difference in mice fed the pediocin-negative strain compared to the control group was observed. This is the first demonstration that human L. lactis and P. acidilactici nisin-and pediocin-producing strains can reduce VRE intestinal colonization.

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Section: Methodsmentioning

confidence: 99%

Capacity of Human Nisin- and Pediocin-Producing Lactic Acid Bacteria To Reduce Intestinal Colonization by Vancomycin-Resistant Enterococci

Millette

Cornut

Dupont

et al. 2008

Appl Environ Microbiol

138

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“…Biochem., 72 (8), [2232][2233][2234][2235]2008 Note for novel species and novel bacteriocin-producing strains. [10][11][12] In this report, we describe the identification of a novel type-A(II) lantibiotic produced by S. hominis isolated from a traditional Thai fermented fish product (Pla-ra).…”

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confidence: 99%

Identification of the Nukacin KQU-131, a New Type-A(II) Lantibiotic Produced byStaphylococcus hominisKQU-131 Isolated from Thai Fermented Fish Product (Pla-ra)

Wilaipun

Zendo

Okuda

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Staphylococcus hominis KQU-131, isolated from Thai fermented marine fish, produces a heat stable bacteriocin. Structural and genetic analysis indicated that the bacteriocin is a variant of nukacin ISK-1, a type-A(II) lantibiotic, and we termed the bacteriocin nukacin KQU-131. There were three different amino acid residues between nukacin ISK-1 and nukacin KQU-131, one residue in the leader peptide and the other two in the mature peptide.

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“…There are many kinds of fermented foods in Thailand in which LAB can be found. These include fermented pork meat called ''nham'' [10], fermented fish called ''plaa-som'' [11], and a fermented mixture of fish and white rice called ''som-fak'' [12], to mention only three. LAB are known to produce a variety of antibacterial substances including bacteriocins that can inhibit the growth of undesirable bacteria such as Staphylococcus aureus, Listeria monocytogenes, Bacillus spp., and Clostridium spp.…”

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confidence: 99%

Screening of lactic acid bacteria for bacteriocins by microbiological and PCR methods

Suwanjinda

Eames

Panbangred

2007

Biochem Molecular Bio Educ

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We describe a practical laboratory designed for third-year undergraduate students of Biotechnology as part of a Microbial Physiology and Genetics course. It comprises a five-session laboratory module to screen foods for lactic acid bacteria (LAB) 1 and to test isolated LAB for the presence of bacteriocins. Traditional Thai fermented foods are first screened for bacteriocin-producing LAB using microbiological methods. This is followed by a simple and rapid DNA extraction and by a multiplex polymerase chain reaction (PCR) using three pairs of specific primers to test for the presence or absence of various bacteriocin genes in the isolated LAB. PCR amplicons of 332, 412, and 608 bp indicate the presence of pediocin, enterocin, and nisin genes, respectively, whereas no amplicon band indicates the absence of these bacteriocins. The laboratory provides the students with experience in the use of microbiological and multiplex PCR methods and shows how the molecular biology techniques can be related to their daily lives. The module could easily be adapted to the study of fermented foods from other countries.

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Isolation of nisin-producing Lactococcus lactis WNC 20 strain from nham, a traditional Thai fermented sausage

Cited by 144 publications

References 42 publications

Capacity of Human Nisin- and Pediocin-Producing Lactic Acid Bacteria To Reduce Intestinal Colonization by Vancomycin-Resistant Enterococci

Capacity of Human Nisin- and Pediocin-Producing Lactic Acid Bacteria To Reduce Intestinal Colonization by Vancomycin-Resistant Enterococci

Identification of the Nukacin KQU-131, a New Type-A(II) Lantibiotic Produced byStaphylococcus hominisKQU-131 Isolated from Thai Fermented Fish Product (Pla-ra)

Screening of lactic acid bacteria for bacteriocins by microbiological and PCR methods

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