Isolation of Naegleria fowleri from artificially heated water

Fliermans, C.B.; Tyndall, R. L.; Domingue, Elizabeth L.; Willaert, E

doi:10.1016/0306-4565(79)90019-6

Cited by 17 publications

(10 citation statements)

References 8 publications

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“…In our experience with this and other related studies (Fliermans et al 1980, Tyndall et al 1978, Tyndall et al 1979a, water in excess of 40°C generally yields thermophilic nonpathogenic Naegleria and water never heated above 28 to 30°C yields few thermophi lie Naegleria, while pathogenic Naeglet·ia thus far have often 0 been associated with waters in the 30 to 40 C range. These findings suggest a thermal niche as one factor favorable for the emergence of pathogenic Naegleria.…”

Section: Discussionsupporting

confidence: 66%

Presence of pathogenic amoebae in power plant cooling waters. Final report, October 15, 1977-September 30, 1979. [Naegleria fowleri]

Tyndall¹,

Willaert²,

Stevens³

1981

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Section: Discussionsupporting

confidence: 66%

Presence of pathogenic amoebae in power plant cooling waters. Final report, October 15, 1977-September 30, 1979. [Naegleria fowleri]

Tyndall¹,

Willaert²,

Stevens³

1981

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“…fowleri has been isolated from numerous water sources, especially industrial water with artificially elevated temperatures, swimming pools, thermal springs, and domestic water supplies (Brown et al 1983;Cerva et al 1982;DeJonckheere and Van de Voorde 1977;Fliermans et al 1979;Kasprzak et al 1982;Tyndall et al 1989;Wellings et al 1977). Improved methods of detection are needed in order to prevent recreational use of contaminated water.…”

Section: Discussionmentioning

confidence: 97%

Development of a nested PCR assay to detect the pathogenic free-living amoeba Naegleria fowleri

Réveiller

Cabanes

Marciano‐Cabral

2002

Parasitology Research

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Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis, a fatal disease of the central nervous system that is acquired while swimming or diving in freshwater. A cDNA clone designated Mp2C15 obtained from N. fowleri was used as a probe to distinguish N. fowleri from other free-living amoebae. The Mp2C15 probe hybridized to genomic DNA from pathogenic N. fowleri and antigenically related non-pathogenic N. lovaniensis. Mp2C15 was digested with the restriction enzyme XbaI, resulting in two fragments, Mp2C15.G and Mp2C15.P. Four species of Naegleria and four species of Acanthamoeba were examined for reactivity with Mp2C15.P. Mp2C15.P was specific for N. fowleri and was used in the development of a nested PCR assay which is capable of detecting as little as 5 pg of N. fowleri DNA or five intact N. fowleri amoebae. In summary, a rapid, sensitive, and specific assay for the detection of N. fowleri was developed.

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“…While free-living amoebae are abundant in soil and water, thermal conditions are the best-documented environmental factor enhancing the emergence of pathogenic amoebae. The ability of pathogenic Acanthamoebae and Naegleria to grow at temperatures higher than many nonpathogenic species has been demonstrated in laboratory and field studies [63,75]. This key parameter has been useful in isolating pathogenic free-living amoebae from environmental samples.…”

Section: Interactions Of Legionellamentioning

confidence: 99%

Ecology of Legionella: From data to knowledge with a little wisdom

Fliermans

1996

Microb Ecol

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The respiratory diseases produced by the Legionella genus of bacteria are collectively called Legionellosis. Presently more than 34 species of Legionella have been identified, 20 of which have been isolated from both environmental and clinical sources. The diseases produced by Legionella include the pneumonic form, Legionnaires' disease, and the flu-like form, Pontiac fever. Because the vast majority of Legionellosis is caused by the L.pneumophila species, this bacterium is the thrust of the discussion.Legionella is a global bacterium. The relationship of the bacterium to its environment has told us many things about infectious diseases. Not until Legionellosis and the discovery of its etiologic agent, Legionella, has such a successful modern-day marriage been consummated between the agent and its environment. Nearly two decades have passed since the term Legionellosis found its way into the vocabulary of the scientific journals, the popular press, and courtroom proceedings. Too often the scientific development, engineering implementation, and societal acceptance are disconnected. The focus of scientific research sometimes does not reflect engineering or societal needs and thus contributes little to the solution of immediate and important problems. At other times, scientific knowledge that could contribute to solutions is overlooked because of poor communication between the problem holders, the scientific community, regulatory agencies, the problem makers, and the public.The scope of this paper provides insights on the ecological niche of Legionella, describes the organism's ecological relationships in the natural world, and provides wisdom for effective control of the bacterium for the industrial and user communities.

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Isolation of Naegleria fowleri from artificially heated water

Cited by 17 publications

References 8 publications

Presence of pathogenic amoebae in power plant cooling waters. Final report, October 15, 1977-September 30, 1979. [Naegleria fowleri]

Presence of pathogenic amoebae in power plant cooling waters. Final report, October 15, 1977-September 30, 1979. [Naegleria fowleri]

Development of a nested PCR assay to detect the pathogenic free-living amoeba Naegleria fowleri

Ecology of Legionella: From data to knowledge with a little wisdom

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