Isolation of myelin basic protein-reactive T-cell lines from normal human blood

Burns, James; Rosenzweig, Anthony; Zweiman, Burton; Lisak, Robert P.

doi:10.1016/0008-8749(83)90250-2

Cited by 251 publications

(92 citation statements)

References 28 publications

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“…The concept of antigen-specific tolerance has been apparent for some years. The presence of self-reactive lymphocytes in the blood of healthy individuals (Burns et al, 1983) implies that self-antigen-specific regulatory mechanisms are physiological and prevent pathological autoimmunity. Antigen-specific tolerance operates directly on effector T cells (via apoptosis or anergy) and via Treg cells that secrete anti-inflammatory cytokines or compete with effector T cells at the level of the antigen presenting cell (APC).…”

Section: The Importance and Feasibility Of Antigen Specific Immunothementioning

confidence: 99%

Autoantigen-Specific Immunotherapy

Han¹,

Donelan²,

Reeves³

et al. 2011

Type 1 Diabetes - Pathogenesis, Genetics and Immunotherapy

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Section: The Importance and Feasibility Of Antigen Specific Immunothementioning

confidence: 99%

Autoantigen-Specific Immunotherapy

Han¹,

Donelan²,

Reeves³

et al. 2011

Type 1 Diabetes - Pathogenesis, Genetics and Immunotherapy

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“…The fact that autoreactive cells can be detected in the periphery clearly demonstrates that the thymic selection mechanism responsible for the elimination of autoreactive T cell clones is incomplete. 4,5 In this case, how can we ensure that such autoreactive cells will not be reactivated, promoting a break in tolerance and thus the emergence of autoimmune diseases? In other words, the immune system needs different, redundant features to ensure that potential autoimmune responses do not occur.…”

Section: Introductionmentioning

confidence: 99%

“…5,7 In this context, cells with a cellular response regulation function are fundamental and are also important in the modulation of the processes of eliminating pathogen and tumor antigen. These mechanisms occur with destruction of self tissues, exposure of autoantigens and production of pro-inflammatory cytokines, which, unless regulated, favor the induction and maintenance of autoimmune events.…”

Section: Introductionmentioning

confidence: 99%

“…These soluble substances act in a complex network of regulatory mechanisms designed to ensure the modulation of immunological responses to the various antigens derived from infectious agents, tumors, autoantigens and allergens. Among T cells, several subpopulations exhibit regulation properties for exacerbated inflammatory response, such as IL-10-producing T regs , which suppress some cytotoxic T cell responses in vivo, 5,[7][8][9][10] including: CD8+CD28-T cells, CD56 + T cells, γδ T cells [11][12][13] and CD4 -CD8 -T cells. 13 In addition to T cells, there are other cell subtypes that have been described with such properties.…”

Section: Introductionmentioning

confidence: 99%

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The role of regulatory T cells, interleukin-10 and in vivo scintigraphy in autoimmune and idiopathic diseases – Therapeutic perspectives and prognosis

Falcão

Campos

2017

Rev. Assoc. Med. Bras.

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Previous studies have demonstrated the expression of the CD25 marker on the surface of naturally occurring T cells (T regs ) of mice, which have a self-reactive cellular profile. Recently, expression of other markers that aid in the identification of these cells has been detected in lymphocyte subtypes of individuals suffering of autoimmune and idiopathic diseases, including: CD25, CTLA-4 (cytotoxic T-lymphocyte antigen 4), HLA-DR (human leukocyte antigen) and Interleukin 10 (IL-10), opening new perspectives for a better understanding of an association between such receptors present on the cell surface and the prognosis of autoimmune diseases. The role of these molecules has already been described in the literature for the modulation of the inflammatory response in infectious and parasitic diseases. Thus, the function, phenotype and frequency of expression of the α-chain receptor of IL-2 (CD25) and IL-10 in lymphocyte subtypes were investigated. Murine models have been used to demonstrate a possible correlation between the expression of the CD25 marker (on the surface of CD4 lymphocytes) and the control of self-tolerance mechanisms. These studies provided support for the presentation of a review of the role of cells expressing IL-2, IL-10, HLA-DR and CTLA-4 receptors in the monitoring of immunosuppression in diseases classified as autoimmune, providing perspectives for understanding peripheral regulation mechanisms and the pathophysiology of these diseases in humans. In addition, a therapeutic approach based on the manipulation of the phenotype of these cells and ways of scintigraphically monitoring the manifestations of these diseases by labeling their receptors is discussed as a perspective. In this paper, we have included the description of experiments in ex vivo regulation of IL-10 and synthesis of thio-sugars and poly-sugars to produce radiopharmaceuticals for monitoring inflammation. These experiments may yield benefits for the treatment and prognosis of autoimmune diseases.

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“…However, auto-reactive T cells are also frequently part of the mature immune repertoire of healthy non-MS humans (Burns et al, 1983(Burns et al, , 1986Jingwu et al, 1992;Kerlero de Rosbo et al, 1993;Lindert et al, 1999;Markovic-Plese et al, 1995;Ota et al, 1990) as well as of nonimmunized animals (Schluesener and Wekerle, 1985). Thus, humans often show comparable frequencies of myelin [myelin basic protein (MBP), proteolipid protein (PLP) and myelin oligodendrocyte glycoprotein (MOG)]-reactive T cells in their blood, whether they are MS patients or healthy controls (Burns et al, 1983(Burns et al, , 1986Jingwu et al, 1992;Kerlero de Rosbo et al, 1993;Lindert et al, 1999;Markovic-Plese et al, 1995;Ota et al, 1990). These findings suggest that the more important factor in disease development may be activation; that is a greater frequency of activated myelin-reactive cells in MS patients as compared to healthy individuals (Zhang et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

A new approach for evaluating antigen-specific T cell responses to myelin antigens during the course of multiple sclerosis

Arbour

Holz

Sipe

et al. 2003

Journal of Neuroimmunology

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We used a flow cytometry assay to measure proliferation and cytokine production of self-antigenspecific T cells in individual patients during the clinical course of multiple sclerosis (MS). Myelinassociated oligodendrocytic basic protein (MOBP) was selected for proof of principles in the assay, along with myelin basic protein (MBP) to assess specific activated T cells in 10 MS patients over an 18-month period, in parallel with brain magnetic resonance imaging (MRI) scans and clinical rating scale. A positive correlation occurred between antigen-specific T cell proliferation and interferon-γ production with clinical relapses and MRI lesion activity that was absent when the same patients were in remission.

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Isolation of myelin basic protein-reactive T-cell lines from normal human blood

Cited by 251 publications

References 28 publications

Autoantigen-Specific Immunotherapy

Autoantigen-Specific Immunotherapy

The role of regulatory T cells, interleukin-10 and in vivo scintigraphy in autoimmune and idiopathic diseases – Therapeutic perspectives and prognosis

A new approach for evaluating antigen-specific T cell responses to myelin antigens during the course of multiple sclerosis

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