Isolation of Murine Hair-Inducing Cells Using the Cell Surface Marker Prominin-1/CD133

Abstract

Hair is a mini-organ in which dermal papilla (DP) cells play important roles in hair follicle morphogenesis and formation via interactions with epithelial cells. DP cells have previously been difficult to analyze because of the lack of a specific surface marker. We have demonstrated that prominin-1/CD133 (CD133) is a useful marker for murine DP cells. DP cells express CD133 during the early anagen stage (active growth phase) not only during hair morphogenesis, but also during the growth phase of hairs after birth. Gene expression and flow cytometric analysis revealed that CD133-positive (+) cells in the skin possess the characteristics of DP cells. The CD133(+) cells isolated from embryonic or adult skin-induced new hair follicles in vivo when they were transplanted into nude mice mixed with embryonic epithelial cells, but CD133-negative (-) cells could not. We propose that the CD133 is a novel surface marker useful for collecting DP cells in the anagen stage and for analyzing the function of DP.

Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis

“…Consistent with this, as seen by immunofluorescence (IF) microscopy, we noted a close association of laminin-511 with the DP using a DP-specific marker, CD133 ( Fig. 2C; Ito et al 2007) and a lack of detectable noggin in E16.5 lama5 −/− skin (Fig. 2D).…”

Characterization of CD133+parenchymal cells in the liver: Histology and culture

“…In 1997, CD133 was reported as a marker of hematopoietic progenitor cells, using a novel monoclonal antibody that recognized the CD133 antigen [1][2][3] . Subsequently, it was reported that CD133 was also expressed in epithelial and nonepithelial progenitor cells in murine or human tissues including brain, kidney, prostate, pancreas, and skin [4][5][6][7][8] .…”

From where do Cancer-Initiating Cells Originate?

“…A significant example is provided by the contradictory results generated by using the transmembrane protein CD133 as a stem-cell marker. In numerous studies, monoclonal antibodies to CD133 were defined as appropriate tools to isolate CSC from various tumour types (Barker et al, 2009;Yin et al, 1997;Uchida et al, 2000;Lee et al, 2005;Sagrinati et al, 2006;Richardson et al, 2004;Kordes et al, 2007;Oshima et al, 2007;Sugiyama et al, 2007;Ito et al, 2007). Nonetheless, by generating transgenic mice expressing the LacZ reporter gene under the control of the CD133 promoting sequences, the transmembrane protein was found expressed by mature luminal ductal epithelial cells in adult organs, suggesting that it is not a specific marker of stem-cells (Shmelkov et al, 2008).…”