1980
DOI: 10.1007/bf01538695
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Isolation of multiple classes of mutants of CHO cells resistant to cyclic AMP

Abstract: From mutagenized Chinese hamster ovary (CHO) cells we have isolated, in a single step, 11 independent mutants resistant to the growth-inhibitory effects of 8-Br-cyclic AMP, cholera toxin, and methylisobutylxanthine. Two major classes and several subclasses of mutants were obtained. Mutants from all classes have a normal doubling time. None of the mutants respond to cyclic AMP treatment with increased flattening and elongation as do the parental cells. Members of the first class have an altered protein kinase a… Show more

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Cited by 91 publications
(51 citation statements)
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“…All ofthe effects ofcAMP seem to be mediated through its activation of cAMP-dependent protein kinase (30,31 32). Although these studies provide some guidance as to the possible physiological effects of EF, the responses of particular tissues and animals remain to be determined.…”
Section: Resultsmentioning
confidence: 99%
“…All ofthe effects ofcAMP seem to be mediated through its activation of cAMP-dependent protein kinase (30,31 32). Although these studies provide some guidance as to the possible physiological effects of EF, the responses of particular tissues and animals remain to be determined.…”
Section: Resultsmentioning
confidence: 99%
“…CHO cells as well as Lec2 and Lec8 mutant CHO cells were cultured as described previously (21). Cell extracts from neuroblastoma cell lines (SKNAS and SKNSH) were gifts from Dr. Maria Tsokos, NCI, National Institutes of Health.…”
Section: Methodsmentioning
confidence: 99%
“…For some experiments, large quantities of cells were grown in suspension by using 100-ml glass bottles containing 50 ml of complete medium and incubated in a gyratory shaker (New Brunswick Scientific Co., Inc.) at 37°C and 160 rpm. The growth rates of cells in culture (11) and the drug resistance of various cell lines (5) were measured as previously described. 4 ,ug of taxol per ml, 5% dimethyl sulfoxide, and 0.1% aprotinin.…”
Section: Methodsmentioning
confidence: 99%