Isolation of mononuclear cytotoxic cells from cattle vaccinated against foot-and-mouth disease

Abstract

Mononuclear cells from peripheral blood leucocytes of foot-and-mouth disease (FMD) vaccinated cattle underwent blast transformation after in vitro culture with purified, inactivated, 146 S FMD virus antigen. From the prolonged culture of blast cells in medium with Interleukin-2 (5-10 U/ml), CD 45-positive effector cells were derived, which showed a potent, non MHC-restricted activity against virus-infected cells, the extent of which was inversely correlated with multiplicity of infection (MOI). Extensive characterization of effector cells by means of monoclonal antibodies (MAbs) in flow cytometry, lysis of various cell populations with MAbs and complement, Fc receptor analysis and 51Cr release assays indicated that the isolated cells share some phenotypic and functional features of the human and murine Large Granular Lymphocyte/Natural Killer (N.K.) lineage.

Cell mediated innate responses of cattle and swine are diverse during foot-and-mouth disease virus (FMDV) infection: A unique landscape of innate immunity

“…Further studies should precisely define particular phenotypes responsible for both NK-like behavior and antigen presenting properties and particularly if these cells are able to activate CD4 + T cells in an antigen-dependent manner. Our data support the earlier findings of Amadori et al [53,54] and suggest an important role of ␥␦ T cells in the innate immune response against FMDV. In cattle persistently infected with bovine leukemia virus (BLV), administration of recombinant IFN␥ increased the number of ␥␦ T cells in circulation and suppressed the replication of BLV in vivo, although no mechanisms were suggested by these authors on how the ␥␦ T cells eliminated the IgM + cells harboring the virus [57].…”

“…In a study perfomed by Amadori et al [53] a population of cells with a phenotype and function similar to the human or murine large granular lymphocytes/NK lineage was indicated. Those cells, isolated from peripheral blood of cattle vaccinated against FMDV, could lyse target cells infected with virus in an MHC-independent manner.…”

Natural killer (NK) cells in domestic animals: Phenotype, target cell specificity and cytokine regulation

“…T19 + cells are gamma/delta + T-cells in sheep and cattle. These studies clearly indicate that fresh bovine PBM NK-like cells only lyse targets (in 18-20 h cytotoxicity assays) that express herpes virus, foot and mouth virus (Amadori et al, 1992), parainfluenza or bovine leukaemia virus determinants (Table VII). The exception is that LAK-like cells, as with their murine and human counterparts, have a more extensive repertoire of sensitive target cells.…”

Characterization of a Distinct Subpopulation of Bovine γδ T cells

“…These had been previously characterized as a surface Ig -, C D 2 -, C D 4 -, CD8 -, workshop cluster 1 (WCI) -, yS TcR (86D epitope) -, CD5 -, C D 6 -, CD45 +, C D 3 +, FcyR +, mononuclear, cytotoxic cell population of cattle (AMADORI et al, 1992a(AMADORI et al, , 1992b. This intriguing antigenic phenotype prompted investigation of the issue with a further panel of mAbs in FCM analyses.…”

“…U.S. Copyright Clearance Center Code Statement: 0931 -1793 /95/4203 -0162$11.00 /o Among bovine cytotoxic lymphocytes, the isolation of mononuclear, non-adherent, major histocompatibility complex (MHC)-unrestricted cytotoxic cells from cattle vaccinated against foot-and-mouth disease has previously been reported (AMADORI et al, 1992a, b); these lymphocytes were shown to reduce considerably foot-and-mouth disease virus (FMDV) replication in both fetal bovine kidney (AMADORI et al, 1992a) and calf kidney cells, to be amplified in wivo during bovid herpesvirus 1 infection (AMADORI, M., submitted for publication) and to recognize aspects of 'missing self' (LJUNGGREN and KARRE, 1990) on virus-infected target cells (AMADORI et al, 1992b). In this article these cytotoxic lymphocytes are characterized as a distinct population of yS T cells, the distribution in wivo of which is analysed.…”