Isolation of microsatellite markers in the tiger mosquito <i>Aedes albopictus</i> (Skuse)

Porretta, Daniele; Gargani, Maria; Bellini, Romeo; Calvitti, Maurizio; Urbanelli, Sandra

doi:10.1111/j.1471-8286.2006.01384.x

Cited by 28 publications

(28 citation statements)

References 7 publications

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“…albopictus [26], one (AealbB52, Table 2) was not variable and the genetic resolution obtained using the remaining five markers was not considered adequate. We screened some Ae.…”

Section: Methodsmentioning

confidence: 99%

Evidence of Habitat Structuring Aedes albopictus Populations in Réunion Island

et al. 2013

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Arbovirus vector dynamics and spread are influenced by climatic, environmental and geographic factors. Major Chikungunya and Dengue fever outbreaks occurring the last 10 years have coincided with the expansion of the mosquito vector Aedes albopictus to nearly all the continents. We characterized the ecological (larval development sites, population dynamics, insemination and daily survival rates) and genetic (diversity, gene flow, population structure) features of two Aedes albopictus populations from distinct environments (rural and urban) on Réunion Island, in the South-West Indian Ocean. Microsatellite analysis suggests population sub-structuring Ae. albopictus populations. Two genetic clusters were identified that were significantly linked to natural versus urban habitats with a mixed population in both areas. Ae. albopictus individuals prefer urban areas for mating and immature development, where hosts and containers that serve as larval development sites are readily available and support high population densities, whereas natural environments appear to serve as reservoirs for the mosquito.

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“…albopictus [26], one (AealbB52, Table 2) was not variable and the genetic resolution obtained using the remaining five markers was not considered adequate. We screened some Ae.…”

Section: Methodsmentioning

confidence: 99%

Evidence of Habitat Structuring Aedes albopictus Populations in Réunion Island

et al. 2013

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“…For mosquitoes, six SSRs were isolated in Ae. albopictus using an enriched genomic library technique (Porretta et al, 2006), and 20, 21 and 13 SSRs were identified in An. minimus, An.…”

Section: Ssr Numbers In 23 Mosquito Species Genomesmentioning

confidence: 99%

“…dirus (Bonizzoni et al ., ), An. maculatus (Rongnoparut et al ., ) and Aedes albopictus (Porretta et al ., ). The SSRs of An.…”

Section: Introductionmentioning

confidence: 99%

Comparative analyses of simple sequence repeats (SSRs) in 23 mosquito species genomes: Identification, characterization and distribution (Diptera: Culicidae)

et al. 2018

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Simple sequence repeats (SSRs) exist in both eukaryotic and prokaryotic genomes and are the most popular genetic markers, but the SSRs of mosquito genomes are still not well understood. In this study, we identified and analyzed the SSRs in 23 mosquito species using Drosophila melanogaster as reference at the whole-genome level. The results show that SSR numbers (33 076-560 175/genome) and genome sizes (574.57-1342.21 Mb) are significantly positively correlated (R = 0.8992, P < 0.01), but the correlation in individual species varies in these mosquito species. In six types of SSR, mono- to trinucleotide SSRs are dominant with cumulative percentages of 95.14%-99.00% and densities of 195.65/Mb-787.51/Mb, whereas tetra- to hexanucleotide SSRs are rare with 1.12%-4.22% and 3.76/Mb-40.23/Mb. The (A/T)n, (AC/GT)n and (AGC/GCT)n are the most frequent motifs in mononucleotide, dinucleotide and trinucleotide SSRs, respectively, and the motif frequencies of tetra- to hexanucleotide SSRs appear to be species-specific. The 10-20 bp length of SSRs are dominant with the number of 110 561 ± 93 482 and the frequency of 87.25% ± 5.73% on average, and the number and frequency decline with the increase of length. Most SSRs (83.34% ± 7.72%) are located in intergenic regions, followed by intron regions (11.59% ± 5.59%), exon regions (3.74% ± 1.95%), and untranslated regions (1.32% ± 1.39%). The mono-, di- and trinucleotide SSRs are the main SSRs in both gene regions (98.55% ± 0.85%) and exon regions (99.27% ± 0.52%). An average of 42.52% of total genes contains SSRs, and the preference for SSR occurrence in different gene subcategories are species-specific. The study provides useful insights into the SSR diversity, characteristics and distribution in 23 mosquito species of genomes.

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“…Although the previous studies provided important insights into the possible origin of the invasions, their results were sometimes contradictory [e.g., the case of Greece (Kamgang et al, 2011;Manni et al, 2017) or Brazil (Birungi & Munstermann, 2002;Kambhampati, Black, & Rai, 1991)]. However, determining the origin of the invasions unequivocally and/or at a high level of resolution would be valuable for a variety of public health interventions (Beebe et al, 2013;Delatte et al, 2013;Galtier, Nabholz, Glemin, & Hurst, 2009;Goubert, Minard, Vieira, & Boulesteix, 2016;Hurst & Jiggins, 2005;Manni et al, 2015;Medley, Jenkins, & Hoffman, 2015;Mousson et al, 2005;Porretta, Gargani, Bellini, Calvitti, & Urbanelli, 2006;Zhong et al, 2013). First, knowledge regarding the source of an introduction's origin(s) can provide information on the invasive population's likely mode of transportation (Goubert et al, 2016;Jackson et al, 2015;Powell & Tabachnick, 2013).…”

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confidence: 99%

Population genomics of the Asian tiger mosquito, Aedes albopictus: insights into the recent worldwide invasion

Kotsakiozi

Richardson

Pichler

et al. 2017

Ecology and Evolution

111

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Aedes albopictus, the “Asian tiger mosquito,” is an aggressive biting mosquito native to Asia that has colonized all continents except Antarctica during the last ~30–40 years. The species is of great public health concern as it can transmit at least 26 arboviruses, including dengue, chikungunya, and Zika viruses. In this study, using double‐digest Restriction site‐Associated DNA (ddRAD) sequencing, we developed a panel of ~58,000 single nucleotide polymorphisms (SNPs) based on 20 worldwide Ae. albopictus populations representing both the invasive and the native range. We used this genomic‐based approach to study the genetic structure and the differentiation of Ae. albopictus populations and to understand origin(s) and dynamics of the recent invasions. Our analyses indicated the existence of two major genetically differentiated population clusters, each one including both native and invasive populations. The detection of additional genetic structure within each major cluster supports that these SNPs can detect differentiation at a global and local scale, while the similar levels of genomic diversity between native and invasive range populations support the scenario of multiple invasions or colonization by a large number of propagules. Finally, our results revealed the possible source(s) of the recent invasion in Americas, Europe, and Africa, a finding with important implications for vector‐control strategies.

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Isolation of microsatellite markers in the tiger mosquito Aedes albopictus (Skuse)

Cited by 28 publications

References 7 publications

Evidence of Habitat Structuring Aedes albopictus Populations in Réunion Island

Evidence of Habitat Structuring Aedes albopictus Populations in Réunion Island

Comparative analyses of simple sequence repeats (SSRs) in 23 mosquito species genomes: Identification, characterization and distribution (Diptera: Culicidae)

Population genomics of the Asian tiger mosquito, Aedes albopictus: insights into the recent worldwide invasion

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