Isolation of micropropagated strawberry endophytic bacteria and assessment of their potential for plant growth promotion

Dias, Armando Cavalcante Franco; Costa, Francisco Eduardo de Carvalho; Andreote, Fernando Dini; Lacava, Paulo Teixeira; Teixeira, Manoel Araújo; Assumpção, Laura C.; Araújo, Welington Luiz; Azevedo, João Lúcio; Melo, I. S. de

doi:10.1007/s11274-008-9878-0

Cited by 172 publications

(106 citation statements)

References 29 publications

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“…In general, all growth was more accentuated in the first 30 days. As claimed by Dias et al (2009), the initial growth can be attributed to the metabolic substances produced after the bacterial inoculation. Under incubator conditions, the microorganisms Bacillus spp and Sphingopyxis sp.…”

Section: Discussionmentioning

confidence: 86%

Isolation, Characterization and Selection of Bacteria that Promote Plant Growth in Grapevines (Vitis sp.)

Biasolo¹,

Kucmanski²,

Salamoni³

et al. 2016

JAS

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Certain bacteria can promote and stimulate plant growth, increasing the production of biomass and reducing damage caused by phytopathogens. With that in mind, this research effort set out to select these plant growth-promoting bacteria in order to evaluate their effects on the growth of grapevines (Vitis sp.). The bacteria were isolated from several vineyard soil samples, and evaluated based on their production of IAA (Indole-3-Acetic Acid), siderophores and cellulase, as well as their phosphate solubilization and nitrogen fixation capabilities. In vivo testing included six separate treatments with the following bacterial isolates: C12, O7, B3, I3, a control group and a blended group. The tests were performed in a greenhouse with bacterial suspension inoculation placed around the roots of Paulsen 1103 rootstock cuttings. The data collected included the following: number of leaves per plant, branch lengths, chlorophyll content, fresh and dry mass, and Carbon, Hydrogen, Nitrogen and Sulfur concentrations. Forty-six separate bacteria were isolated, of which 100% produced IAA, 65.21% produced siderophores, 63.04% solubilized phosphate, 34.78% produced cellulase, and 30.43% showed nitrogen fixation. The in vivo testing also revealed significant increases in the length of the branch and in percentages of Carbon and Nitrogen. The C12 isolate exhibited the highest increase in branch length (76.704 cm), whereas the O7 and C12 were identified as Bacillus amyloliquefaciens and Bacillus thuringiensis, respectively.

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Section: Discussionmentioning

confidence: 86%

Isolation, Characterization and Selection of Bacteria that Promote Plant Growth in Grapevines (Vitis sp.)

Biasolo¹,

Kucmanski²,

Salamoni³

et al. 2016

JAS

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“…In tropical soils, this P is rapidly fixed. Hence, one way of minimizing these losses is the application of inoculants containing bacteria that can solubilize this P and transform it into forms assimilable by plants, resulting in better use and crop yield (Wakelin et al, 2004;Canbolat et al, 2006;Dias et al, 2009), and contributing to sustainability.…”

Section: Discussionmentioning

confidence: 99%

Bacteria associated with sugarcane in Northeastern Brazil

Silva¹,

Freire²,

Kuklinsky-Sobral³

et al. 2016

Afr. J. Microbiol. Res.

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Sugarcane has a high demand in nitrogen, increasing costs and causing damages to the environment. It is necessary to find alternatives to reduce nitrogen fertilizers use. Diazotrophic bacteria have capacity to promote growth in grass with potential to fix N 2 and solubilize inorganic phosphate. This study aimed to evaluate bacterial community associated with different sugarcane varieties in Northeastern Brazil, select bacteria with plant growth-promoting characteristics, and identify endophytic and epiphytic bacterial lineages in sugarcane. Endophytic bacteria of leaves and roots and epiphytic bacteria of rhizoplane were isolated from three sugarcane commercial varieties and selected for their capacity to fix N 2 and solubilize inorganic phosphate. Bacterial strains from different morphological groups were isolated and a sample of 27 strains with potential for the simultaneous development of these characteristics were selected and identified. The bacterial community that interacted with sugarcane was more associated with rhizoplane and roots regions than with leaves, and had a high potential to fix N 2 and solubilize inorganic phosphate. Bacterial lineages were mainly from genera Pantoea sp. and Burkholderia sp., but there were also genera Enterobacter sp., Klebsiella sp. and Pseudomonas sp. and two lineages at the species level: Pantoea stewartii and Burkholderia cenocepacia.

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“…Our data suggest that the isolates recovered from in vitro yacon cultures were endophytes except for F. oryzihabitans, which grew endo and epiphytically. Several reports indicate that plant growth promoting bacteria are beneficial endophytes growing in association with the host [21][22][23]. Among the S. sonchifolius isolates, Microbacterium imperiali and Sphingomonas paucimobilis were the highest IAA producers ( Table 4).…”

Section: Isolation and Identification Of Epiphytic And Endophytic Bacmentioning

confidence: 96%

Bacterial Community Associated with Autotrophic and Heterotrophic Cultures of Medicinal Plant Smallanthus sonchifolius (Yacon)

Moraes¹,

Melo²,

Sumyanto³

et al. 2012

AJPS

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Several unfruitful attempts to grow axenic shoot cultures of Smallanthus sonchifolius, also known as yacon, were made before healthy shoots grew in association with bacteria on half strength Murashigue and Skoog media supplemented with 2.2 µM benzylaminopurine. Twenty-one bacterial isolates were obtained from in vitro S. sonchifolius plantlets, eight of these isolates were identified as Flavimonas oryzihabitans, Curtobacterium pusillum, Sphingomonas paucimobilis, and Microbacterium imperiale. These microorganisms produced indole acetic acid (IAA) at amounts varying between 8.89 to 47.45 μg/mL, reason for being classified as plant growth promoting bacteria (PGPB). The results show that buds associated with bacteria cultured on sucrose free media produced 3.77 new roots measuring 18.33 cm in length after a 30-day growing period. In contrast, buds growing on sucrose supplemented media, the number of roots induced was higher (6.67 to 14 roots/explant) but shorter in length, 4.67 to 5.83 cm. During plant acclimatization to soil, photosynthesis and water efficiency were measured showing that the plants were healthy and vigorous. A slightly higher rate of photosynthesis and water use efficiency was recorded in the plants produced on heterotrophic conditions as compared to plants grown in sucrose free media. Plants adapted well in the soil demonstrating that the PGPB community associated to S. sonchifolius in shoot cultures was not harmful to plant production. The purpose of this study was to demonstrate that the bacteria associated with in vitro S. sonchifolius shoot cultures was not the result of microbial contamination, but rather from symbiotic associations that extended from cultivation in the greenhouse, to culture and back to soil. This is the first report to show that autotrophic cultures may represent a viable alternative to grow healthy plants without eliminating beneficial bacteria associated with the host.

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Isolation of micropropagated strawberry endophytic bacteria and assessment of their potential for plant growth promotion

Cited by 172 publications

References 29 publications

Isolation, Characterization and Selection of Bacteria that Promote Plant Growth in Grapevines (Vitis sp.)

Isolation, Characterization and Selection of Bacteria that Promote Plant Growth in Grapevines (Vitis sp.)

Bacteria associated with sugarcane in Northeastern Brazil

Bacterial Community Associated with Autotrophic and Heterotrophic Cultures of Medicinal Plant Smallanthus sonchifolius (Yacon)

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