Isolation of Microalgae from Mediterranean Seawater and Production of Lipids in the Cultivated Species

Abstract

Isolation and identification of novel microalgae strains with high lipid productivity is one of the most important research topics to have emerged recently. However, practical production processes will probably require the use of local strains adapted to commanding climatic conditions. The present manuscript describes the isolation of 96 microalgae strains from seawater located in Bay M’diq, Morocco. Four strains were identified using the 18S rDNA and morphological identification through microscopic examination. The biomass and lipid productivity were compared and showed good results for Nannochloris sp., (15.93 mg/L/day). The lipid content in the four species, namely Nannochloropsis gaditana, Nannochloris sp., Phaedactylum tricornutum and Tetraselmis suecica, was carried out by high performance liquid chromatography coupled to mass spectrometry (HPLC-MS )highlighting the identification of up to 77 compounds.

Distribution, screening and biochemical characterization of indigenous microalgae for bio-mass and bio-energy production potential from three districts of Pakistan

“…Characterization of microalgae for bio-mass & bio-energy hence no growth was observed. Micropipette washing technique unethical for isolation of large number of isolates (Haoujar et al, 2020); however, spread plate method for isolation of microalgae is convenient and less laborious to isolate large number of microalgae. Blue green 11 (BG-11) medium was utilized for the isolation of microalgae; different media were analyzed by researcher for isolation of microalgae i.e., Bolds Basic medium, F/2 and K medium.…”

Isolation and Culturing Axenic Microalgae: Mini–Review

“…Essentially, samples are separated into two portions mainly based on particle size differences [7]. Recently, Haoujar (2020) applied this method by filtering samples from Moroccan Mediterranean seawater through a series of membranes decreasing mesh (33, 20, and 0.45 μm) then placing membranes on agar plates, thus achieving the isolation of 32 microalgae (Mostly coccoid) [9]. This method allows the separation of microorganisms by their cell size only, but by itself, it is usually not enough to obtain axenic or unialgal cultures since different organisms can have similar cell sizes.…”

Bioprospecting of Microalgae Isolated from the Adriatic Sea: Characterization of Biomass, Pigment, Lipid and Fatty Acid Composition, and Antioxidant and Antimicrobial Activity

“…Furthermore, implementing those processes contributes to a sustainable energy supply, food security, creates new jobs, and stimulates economic growth [14][15][16]. The bioactive compounds that can be found in microalgae include polyphenols, carotenoids, polysaccharides, ω-3 fatty acids, and polyunsaturated fatty acids (PUFA) [17][18][19][20][21][22][23]. The lipid content in microalgae ranges between 20% to 70%.…”

“…The lipid content in microalgae ranges between 20% to 70%. Microalgal lipids are generally considered a valuable source of polyunsaturated fatty acids (PUFAs), including ω-3 (eicosapentaenoic acid (C20:5), docosahexaenoic acid (C22:6), α-linolenic (C18:3 (ω-3)) and ω-6 (linoleic (C18:2), and γ-linolenic (C18:3 (ω-6)) fatty acids [22,24]. Diatoms, microalgae belonging to the division Bacillariophyceae, are an important source of eicosapentaenoic acid with 15% to 30% in total lipids [18,23].…”