Isolation of methicillin-resistant <i>Staphylococcus aureus</i>
 from small ruminants and their meat at slaughter and retail level in Greece

Sergelidis, Daniel; Papadopoulos, T.; Komodromos, Dimitrios; Sergelidou, E.; Lazou, Thomai; Papagianni, Maria; Ζdragas, Αntonios; Papa, Anna

doi:10.1111/lam.12485

Cited by 16 publications

(20 citation statements)

References 33 publications

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“…S. aureus and MRSA isolation rates from animals and carcass’ surfaces observed in this study are in accordance with another recent study from Greece conducted with samples from small ruminants and their carcasses at slaughter and retail level (Sergelidis et al, ). However, our results are lower as compared to data from Velasco, Sherwood, Rojas‐Garcia, and Logue () (40.6%) and Waters et al (), who revealed S. aureus prevalence of 47% in 136 samples of raw meat and poultry.…”

Section: Resultssupporting

confidence: 93%

“…Multiple studies demonstrating the high prevalence of multidrug-resistant S. aureus, including ST398, among livestock in the European Union, Canada, and United States have been reported (Khanna, Friendship, Dewey, & Weese, 2008;Smith & Pearson, 2011). In Greece, even though S. aureus clones disseminated in the community and hospitals among humans are well characterized, limited data are available concerning clonal spread in food production chain (including animals, equipment, meat products, abattoir personnel) that may influence the epidemiology of staphylococcal infections in the community and hospital environments (Drougka et al, 2016(Drougka et al, , 2014(Drougka et al, , 2012Sarrou et al, 2015;Sergelidis et al, 2015).…”

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confidence: 99%

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Multidrug‐resistant enterotoxigenicStaphylococcus aureuslineages isolated from animals, their carcasses, the personnel, and the environment of an abattoir in Greece

Δρούγκα

Foka

Giormezis

et al. 2019

J Food Process Preserv

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This study investigated genetic relatedness among Staphylococcus aureus from livestock, their carcass’ surfaces after slaughter, personnel, and equipment of a Greek abattoir. Genotyping was performed using PFGE, MLST, spa, and SCCmec. Antimicrobial susceptibility was determined according to EUCAST guidelines. Genes encoding staphylococcal enterotoxins (SEs) and Panton‐Valentine leukocidin (PVL) were detected by PCRs. From 392 samples, 46 S. aureus were recovered, 10 from workers, 6 from animals, 10 from carcass’, and 20 from equipment’s surfaces. Resistance to tetracycline was 80.4%, whereas, 58.7% of isolates were multi‐drug resistant. Eleven isolates (23.9%) were MRSA, 8 mecA‐ and 3 mecC‐positive. Fifteen PFGE and 13 spa types were identified, classified in 10 sequence types (STs). ST80 predominated among MSSA, and ST88‐IV clone among MRSA. Fifteen strains (32.6%) carried enterotoxin genes 7 of them possessed also the PVL genes. The abattoir seems to play important role in the transmission of S. aureus between animals and humans. Practical applications Antibiotic susceptibility, clonality, and toxin profiles among Staphylococcus aureus recovered from humans, animals, resulting carcasses and environmental surfaces of an abattoir were assessed. Our results showed spread of multi‐resistant toxigenic strains belonging to human and animals’ origin clones, indicating that the abattoir might be the reservoir for their transmission with potential negative impact on consumers’ health. The establishment and implementation of strict hygiene rules in the farms and during the slaughtering process in the abattoirs could control spread of virulent S. aureus strains.

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Section: Resultssupporting

confidence: 93%

mentioning

confidence: 99%

Multidrug‐resistant enterotoxigenicStaphylococcus aureuslineages isolated from animals, their carcasses, the personnel, and the environment of an abattoir in Greece

Δρούγκα

Foka

Giormezis

et al. 2019

J Food Process Preserv

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“…However, closely related or identical isolates were detected in different farms and probably this is evidence that certain genotypes dominate. Same results had been published from France (Vautor et al 2003), from Spain and Italy (Goñi et al 2004;Spanu et al 2013) and recently from Greece (Zdragas et al 2015). Diversity in epidemiologically unrelated strains has been observed several times (Campos et al 2009; Otero et al 2013; Zdragas et al 2015) and the same was observed in this study.…”

Section: Discussionsupporting

confidence: 89%

“…It is interesting that 83% of S. aureus isolates were susceptible to all the antimicrobials tested. The penicillin resistance level of 17% is similar to some other reports (Mørk et al 2005;Zdragas et al 2015) but low compared with the 96•3% elsewhere (Gündogȃn et al 2006). This is a strong evidence of the low pressure of antibiotics in sheep and goat industry in Greece.…”

Section: Discussionsupporting

confidence: 84%

“…Genotyping. Pulse Field Gel Electrophoresis (PFGE) was performed for S. aureus strains using SmaI (Takara, Tokyo, Japan) as restriction endonuclease as described previously (Sergelidis et al 2015) and for E. coli strains according to Milk microbiological profiles in conventional and organic farming systems Pulsenet protocol (Ribot et al 2006) with slight modifications. Briefly, S. aureus strains were grown overnight in 5 ml Brain Heart Infusion broth at 37°C, while 200 µl harvested by centrifugation, washed with TE buffer (10 mM Tris:1 mM EDTA, pH 8•0) and finally suspended to 300 µl TE buffer.…”

Section: Sample Analysismentioning

confidence: 99%

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Differences in sheep and goats milk microbiological profile between conventional and organic farming systems in Greece

Malissiova

Papadopoulos

Kyriazi

et al. 2017

Journal of Dairy Research

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The aim of this study was to examine differences in the microbiological profile and antimicrobial resistance of bacteria isolated from milk from organic and conventional sheep and goat farms. Twenty-five organic and 25 conventional sheep and goat farms in the region of Thessaly, Greece participated in this study. A standardised detailed questionnaire was used to describe farming practices. A total of 50 samples were collected and analysed for total viable count (TVC), total coliform count (TCC) and somatic cell count (SCC), while Staphylococcus aureus and Escherichia coli were isolated using standard methods. Isolates were identified at species level by Api-test and Matrix-Assisted Laser Desorption/Ionisation-Time of Flight Mass Spectrometry (MALDI-TOF MS). Susceptibility to a panel of 20 for E. coli and 16 for S. aureus antimicrobials was determined by the agar dilution method. Pulsed Field Gel Electrophoresis (PFGE) was performed for S. aureus and E. coli isolates to determine predominant clones. Lower counts of TVC, TCC and SCC were identified in milk from the organic farms, possibly due to differences in the hygienic farming practices found on those farms. API-tests and MALDI-TOF MS showed no significant differences in the S. aureus and E. coli isolates. Overall, antimicrobial resistance rates were low, while a statistically higher percentage was estimated among strains originating from conventional farms in comparison with organic farms, possibly due to the restriction of antibiotic use in organic farming. PFGE revealed diversity among S. aureus and E. coli populations in both organic and conventional farms indicating circulation of 2-3 main clones changing slightly during their evolution. Consequently, there is evidence that milk from the organic farms presents a better microbiological profile when compared with milk from conventional farms.

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Role of phage-antibiotic combination in reducing antibiotic resistance in Staphylococcus aureus

Kim

Ding

et al. 2016

Food Sci Biotechnol

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This study was designed to evaluate the effect of phage-antibiotic synergy in reducing antibiotic resistance. The initial numbers of treated with ciprofloxacin, phages, and combination were significantly reduced by 3.47, 4.62, and 5.75 log CFU/mL, respectively, at the early 12 h of incubation. The combination treatment most effectively inhibited the growth of, showing more than 4 log reduction in 18 h of incubation at 37°C. The significant reduction in biofilm formation by was observed at the combination treatment (3.91 log). Ciprofloxacin-treated cells became resistant to both ciprofloxacin and phage, showing the mutant frequencies of 27% and 25%, respectively, whereas no antibiotic- and phage-resistant cells were observed at the combined treatment of ciprofloxacin and phages. These results provide useful information for reducing the risk of antibiotic resistance in human and food animals.

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Isolation of methicillin-resistant Staphylococcus aureus from small ruminants and their meat at slaughter and retail level in Greece

Cited by 16 publications

References 33 publications

Multidrug‐resistant enterotoxigenicStaphylococcus aureuslineages isolated from animals, their carcasses, the personnel, and the environment of an abattoir in Greece

Multidrug‐resistant enterotoxigenicStaphylococcus aureuslineages isolated from animals, their carcasses, the personnel, and the environment of an abattoir in Greece

Differences in sheep and goats milk microbiological profile between conventional and organic farming systems in Greece

Role of phage-antibiotic combination in reducing antibiotic resistance in Staphylococcus aureus

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