Isolation of methanogens from Arabian sea sediments and their salt tolerance

Kadam, Priya C.; Godbole, S. H.; Ranade, Dilip R.

doi:10.1111/j.1574-6968.1989.tb03389.x

Cited by 7 publications

(2 citation statements)

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“…The optimum NaCl concentration for KOR-3, -5, and -6 and KOR-4 was 1.0% and 0.5% (w/v), respectively ( Table 1 ). Certain species of Methanosarcina are halophilic or halotolerant in nature, such as Methanosarcina thermophila , which can grow in up to 7.0% (w/v) NaCl, with optimum growth occurring at 3.5% (w/v) NaCl [ 21 ] and M. barkeri A-12 and A-13, which can grow in up to 3% NaCl, with optimum growth occurring at 0.06% NaCl [ 26 ]. Thakker and Ranade [ 27 ] also described isolate LN 1, which grew in 3% NaCl.…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of new Methanosarcina mazei strains KOR-3, -4, -5, and -6 from an anaerobic digester using pig slurry

Battumur¹,

Yoon

Bae

et al. 2017

Asian-Australas J Anim Sci

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ObjectiveAn experiment was conducted to isolate and identify new methanogens in Korea from an anaerobic digester that uses pig slurry.MethodsAn anaerobic digestate sample was collected from an anaerobic digester using pig slurry. Pre-reduced media were used for the growth and isolation of methanogens. Growth temperature range, pH range, NaCl concentration range, substrate utilization, and antibiotic tolerance were investigated to determine the physiological characteristics of isolated methanogens. The isolates were also examined microscopically for their morphology and Gram-stained. Polymerase chain reaction of 16S rRNA and mcrA gene-based amplicons was used for identification purpose.ResultsFour strains, designated KOR-3, -4, -5, and -6, were isolated and were non-motile, irregular coccoid, and 0.5 to 1.5 μm in diameter. Moreover, the cell walls of isolated strains were Gram-negative. KOR-3 and KOR-4 strains used acetate for methane production but did not use H2+CO2, formate, or methanol as a growth substrate KOR-5 and KOR-6 strains utilized acetate, methanol, and trimethylamine for methanogenesis but did not use H2+CO2 or formate as a growth substrate. The optimum temperature and pH for growth of four strains were 39°C and 6.8 to 7.2, respectively. The optimum concentration of NaCl for growth of KOR-3, KOR-5, and KOR-6 were 1.0% (w/v). The optimum NaCl concentration for KOR-4 was 0.5% (w/v). All of the strains tolerated ampicillin, penicillin G, kanamycin, streptomycin, and tetracycline; however, chloramphenicol inhibited cell growth. Phylogenetic analysis of 16S rRNA and mcrA genes demonstrated that strains KOR-3, -4, -5, and -6 are related to Methanosarcina mazei (M. mazei, 99% sequence similarity).ConclusionOn the basis of physiological and phylogenetic characteristics, strains KOR-3, -4, -5, and -6 are proposed to be new strains within the genus Methanosarcina, named M. mazei KOR-3, -4, -5, and -6.

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Section: Resultsmentioning

confidence: 99%

Isolation and characterization of new Methanosarcina mazei strains KOR-3, -4, -5, and -6 from an anaerobic digester using pig slurry

Battumur¹,

Yoon

Bae

et al. 2017

Asian-Australas J Anim Sci

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“…However, these techniques did not solve the problem completely. Some studies on methanogenesis in marine and brackish sediments indicated that there are some species of methanogenic bacteria that are still active at high sodium concentrations ( Compeau and Bartha, 1987 ; Kadam et al, 1989 ). A study by Krongthamchat and Riffat (2001) showed that halophilic methanogens isolated from mangrove soil had high potential for anaerobic degradation of organic pollutants in wastewater containing various salt concentrations (0 to 50 g NaCl/L).…”

Section: Introductionmentioning

confidence: 99%

Anaerobic Treatment of High‐Saline Wastewater Using Halophilic Methanogens in Laboratory‐Scale Anaerobic Filters

Riffat¹,

Krongthamchat²

2007

Water Environment Research

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The presence of a high concentration of sodium in wastewater is considered inhibitory for anaerobic biological treatment. This research was designed to investigate the potential use of halophilic methanogens and a mixed culture of halophilic methanogens and digester sludge, in anaerobic filters, for treatment of organic pollutants in high-saline wastewater at 358C. Data related to startup of the filters are presented. Both halophilic and mixed-culture anaerobic filters were able to operate at a sodium chloride concentration of 35 g/L, at organic loading rates (OLRs) of 6.2 and 5 kg chemical oxygen demand (COD)/m 3 Á d, respectively. The COD removal efficiency was as high as 80%, and the systems were able to maintain a low volatile fatty acids concentration of 500 mg/L. No significant difference in COD removal was observed between the halophilic filter and the mixed-culture filter. Increasing the salt concentration to 37 g/L at an OLR of 3 kg/m 3 Á d caused system failure. Water Environ. Res., 79, 191 (2007).

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Microbe-Microbe Interactions

Dolfing¹,

Gottschal²

1997

Gastrointestinal Microbiology

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Isolation of methanogens from Arabian sea sediments and their salt tolerance

Cited by 7 publications

References 20 publications

Isolation and characterization of new Methanosarcina mazei strains KOR-3, -4, -5, and -6 from an anaerobic digester using pig slurry

Isolation and characterization of new Methanosarcina mazei strains KOR-3, -4, -5, and -6 from an anaerobic digester using pig slurry

Anaerobic Treatment of High‐Saline Wastewater Using Halophilic Methanogens in Laboratory‐Scale Anaerobic Filters

Microbe-Microbe Interactions

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