Isolation of Metarhizium guizhouense and Metarhizium robertsii Strains from Soil-Exposed Amblyomma americanum (Acarina: Ixodidae) from Northwest Arkansas, USA

Goldsmith, Austin; Loftin, Kelly; Steinkraus, Donald C.; Szalanski, Allen L.; Cleary, Dylan; Castrillo, Louela A.

doi:10.1653/024.104.0309

Cited by 3 publications

(3 citation statements)

References 39 publications

(66 reference statements)

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“…An analogous study found that Metarhizium species produced conidia with oval and rod‐like morphologies, as well as pale green pigmentation [15–17]. In contrast, the fungal isolates had conidia that were similar in size and shape to those described by Goldsmith et al [37].…”

Section: Discussionmentioning

confidence: 94%

Pathogenicity of Metarhizium rileyi (Hypocreales: Clavicipitaceae) against Tenebrio molitor (Coleoptera: Tenebrionidae)

Vivekanandhan,

Alahmadi,

Ansari

2024

J Basic Microbiol

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Tenebrio molitor L., also known as the mealworm, is a polyphagous insect pest that infests various stored grains worldwide. Both the adult and larval stages can cause significant damage to stored grains. The present study focused on isolating entomopathogenic fungi from an infected larval cadaver under environmental conditions. Fungal pathogenicity was tested on T. molitor larvae and pupae for 12 days. Entomopathogenic fungi were identified using biotechnological methods based on their morphology and the sequence of their nuclear ribosomal internal transcribed spacer (ITS). The results of the insecticidal activity indicate that the virulence of fungi varies between the larval and pupal stages. In comparison to the larval stage, the pupal stage is highly susceptible to Metarhizium rileyi, exhibiting 100% mortality rates after 12 days (lethal concentration 50 [LC50] = 7.8 × 106 and lethal concentration 90 (LC90) = 2.1 × 1013 conidia/mL), whereas larvae showed 92% mortality rates at 12 days posttreatment (LC50 = 1.0 × 106 and LC90 = 3.0 × 109 conidia/mL). The enzymatic analyses revealed a significant increase in the levels of the insect enzymes superoxide dismutase (4.76–10.5 mg−1) and glutathione S‐transferase (0.46–6.53 mg−1) 3 days after exposure to M. rileyi conidia (1.5 × 105 conidia/mL) compared to the control group. The findings clearly show that M. rileyi is an environmentally friendly and effective microbial agent for controlling the larvae and pupae of T. molitor.

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Section: Discussionmentioning

confidence: 94%

Pathogenicity of Metarhizium rileyi (Hypocreales: Clavicipitaceae) against Tenebrio molitor (Coleoptera: Tenebrionidae)

Vivekanandhan,

Alahmadi,

Ansari

2024

J Basic Microbiol

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“…Los HE son importantes antagonistas de insectos plaga que habitan en el suelo (Uzman et al, 2019), y su presencia ha sido ampliamente estudiada en México en diversos cultivos como cítricos (Galán-Franco et al, 2011) Bravo et al, 2021). En Arkansas, EUA y utilizando como insecto trampa el ácaro Amblyomma americanum (Acarina: Ixodidae), lograron aislar e identificar molecularmente a las especies de M. robertsii y M. guizhouense (Goldsmith et al, 2021); lo que demuestra la capacidad de adaptación de estas dos especies a diferentes condiciones ambientales y hábitats. En este estudio, la mayor abundancia de HE se presentó durante la temporada de lluvias, presentando el 64,7% de las larvas micosadas, seguramente favorecido por la humedad que se presenta en esa época del año, la cual beneficia la germinación de conidios y su propagación en el suelo (Ignoffo, 1992).…”

Section: Resultados Y Discusiónunclassified

Presencia natural de Metarhizium en suelos agrícolas de aguacate (Persea americana Mill.) en Colima, México

Rodríguez-Rodríguez

Neri-Luna

Gallou³

et al. 2023

Manglar

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Avocado (Persea americana Mill.) has become one of the most important fruits in the world, being Mexico the main producer and exporter worldwide, it has led to an accelerated growth in the establishment of monoculture avocado plantations, promoting the increase of insect and mite pests, which has led to the need for biological control strategies for integrated pest management. The objective of this study was to isolate entomopathogenic fungi from the soil of a conventional avocado orchard and identify them by translation elongation factor 1-α (5’TEF). During the sampling, a total of 34 isolates were obtained, belonging to the genus Metarhizium, 23 isolates identified as Metarhizium robertsii and 11 as Metarhizium guizhouense. These results show the low diversity of entomopathogenic fungi species associated with the rhizosphere of the avocado crop and suggest that their occurrence could be associated with their ability to survive agronomic practices that induce their endophytic activity. It is necessary to understand the role of Metarhizium in the rhizosphere, this will allow the development of biological control programs specific for each crop, and thus guarantee its success in pest control.

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“…To accomplish this objective, un-engorged A. americanum adults and nymphs of both sexes were obtained from sheep and rabbit hosts housed at the Oklahoma State University (OSU) Tick Rearing Facility, Stillwater, OK. A native isolate of M. robertsii, Savoy P10N1 (ARSEF 14329), was sub-cultured from an infected A. americanum inoculated from a preliminary bioassay. Savoy P10N1, hereafter called P10N1, was isolated from a field-collected tick exposed to soil collected from the Savoy Beef Cattle Research Complex in Savoy, AR (36.128°N, 94.333°W) (Goldsmith et al 2021). Conidia of P10N1 were suspended in a solution of 0.05% Tween 80, 1.5 mg/L penicillin G, and 2.5 mg/L streptomycin.…”

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confidence: 99%

Laboratory Bioassays of a Native Arkansas Isolate of Metarhizium robertsii (Hypocreales: Clavicipitaceae) for the Control of Amblyomma americanum (Acarina: Ixodidae)

Goldsmith

Loftin

Steinkraus

et al. 2023

Journal of Agricultural and Urban Entomology

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Isolation of Metarhizium guizhouense and Metarhizium robertsii Strains from Soil-Exposed Amblyomma americanum (Acarina: Ixodidae) from Northwest Arkansas, USA

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 3 publications

References 39 publications

Pathogenicity of Metarhizium rileyi (Hypocreales: Clavicipitaceae) against Tenebrio molitor (Coleoptera: Tenebrionidae)

Pathogenicity of Metarhizium rileyi (Hypocreales: Clavicipitaceae) against Tenebrio molitor (Coleoptera: Tenebrionidae)

Presencia natural de Metarhizium en suelos agrícolas de aguacate (Persea americana Mill.) en Colima, México

Laboratory Bioassays of a Native Arkansas Isolate of Metarhizium robertsii (Hypocreales: Clavicipitaceae) for the Control of Amblyomma americanum (Acarina: Ixodidae)

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