Isolation of Male Sterile and Maintainer Lines from North-Indian Onion <i>(Allium cepa</i> L.) Populations with the Aid of PCR-Based Molecular Marker

Malik, Geetika; Dhatt, Ajmer Singh; Malik, Ajaz Ahmad

doi:10.5958/2229-4473.2017.00142.2

Cited by 5 publications

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“…Whereas the male sterility maintainer plants (N msms ) were noted at frequencies of 0.133 in Punjab Naroya, 0.231 in Punjab Selection and 0.182 in Punjab, frequencies were estimated by the aid of cob marker (Malik et al, 2017). The CMS-S, CMS-T and N cytoplasms frequencies were 0.47, 0.28 and 0.25, the Ms and ms allele frequencies were 0.52 and 0.48, respectively, noted by cob, orfA501 and orf725 genes-specific markers, and Ms/ms allelic markers of AcSKP1 and AcPMS1 genes were exploited in the MAS of maintainer lines among Brazilian onion germplasm (Ferreira et al, 2017).…”

Section: Marker-a Ss Is Ted S Elec Ti On (Ma S) Of C Y Topl a S Mi C-g Eni C Male S Terilit Y Line Smentioning

confidence: 99%

“…MAS is effectively used in the isolation of male-sterile, their maintainer plants and fertility restorer lines in large populations, saving resources spent on crop establishment and avoiding the complex of phenotypic screening. Markers linked to Ms/ms and CMS-S, -T genes aid in the recovery of male-sterile traits (Malik et al, 2017). The development of F 1 hybrids in onion heterosis breeding was made possible by the use of PCR markers that linked cytoplasms and restorer-of-fertility genes (Amhad et al, 2020).…”

Section: Ms Locusmentioning

confidence: 99%

“…Furthermore, the discovery of molecular markers associated with male fertility restoration (Ms) locus (C/R line) and normal (N) cytoplasm type enables breeders to isolate maintainer lines in any open-pollinated population (Havey, 2013); at seedling stage, we could isolate A-, B-and C/R lines in single season or in a year (Figure 1), whereas conventionally it takes 2-4 years through testcross assay, and advanced to transfer into the desired maintainer background. Transfer of B-line genetic background into an A-line requires 10-12 years through 5-6 backcrosses (Malik et al, 2017); furthermore A-line is crossed to C/R line to produce F 1 hybrids (Figure 1). Thus, the identification of tightly linked markers to the nuclear gene at Ms locus would facilitate easy isolation of maintainer lines by MAS at the seedling stage through screening of seedlings in a limited period, thus reducing expenditure on field screening, labour and crop management inputs (Gorkce et al, 2002).…”

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Perspective and application of molecular markers linked to the cytoplasm types and male‐fertility restorer locus in onion (Allium cepa)

Manjunathagowda

2021

Plant Breeding

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Onion (Allium cepa L.; 2n = 16) belongs to the family alliaceae, and order asparagales; and the Allium is the monocot's largest genus, comprised of 920 species (Govaerts et al., 2005(Govaerts et al., -2014. Among them, onion (A. cepa L.), garlic (A. sativum L.), bunching onion (A. fistulasum L.), leek (A. porrum L.) and chives (A. schenoprosum L.) are widely being in cultivation. Among alliums, the onion has the largest economic potential, with 70% share value, followed by garlic (25%) (FAOSTAT, 2016), the consumption of onions has been steadily increasing over the world, most likely due to the nutritional benefits (Manjunathagowda et al., 2019); presence of an unique dietary flavonoids source and quercetin compounds (Slimestad et al., 2007); and medicinal therapeutic values such as lacrimatic, anti-cancer, antibiotic and anti-cholesterol activity (Grifths et al., 2002; Perez-Gregorio et al., 2010).In the world, onion acreage of 4.96 million hectare (mha) with production of 93.17 million tonnes (mt), the world's biggest onion growers are China (23.91 mt) and India (19.42 mt) (FAOSTAT, 2016).But the productivity of major onion-producing countries, China (22 tonnes per hectare (t/ha)) and India (16.18 t/ha), is low compared to Netherlands (49.70 t/ha) (National Horticultural Board, 2017), the United States (56.40 t/ha), Iran (37.95 t/ha) and Egypt (36.71 t/ha) (National Horticultural Board, 2018). This could be because of the largest onion-growing nations (China and India) have been largely covered by open-pollinated varieties, which produce poor yields per unit area and are influenced by seasonal short-day environmental conditions, as compared to countries that have adopted F 1 hybrids using the male sterility trait

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Section: Marker-a Ss Is Ted S Elec Ti On (Ma S) Of C Y Topl a S Mi C-g Eni C Male S Terilit Y Line Smentioning

confidence: 99%

Section: Ms Locusmentioning

confidence: 99%

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