Isolation of Listeria monocytogenes from the Skin of Slaughtered Beef Cattle

Abstract

ABSTRACT. We attempted to isolate Listeria monocytogenes from skin, contents of large intestines and carcasses of cattle introduced to a slaughterhouse in order to identify source of contamination for this pathogen. Sixty skin samples, 60 samples of the contents of large intestines and 30 carcass samples were colleted in June, August and November 2003 for use in this study. Listeria spp. and L. monocytogenes were isolated from 30 (50%) and 3 (5%) of the cattle skin samples, respectively. However, no Listeria spp., including L. monocytogenes, were isolated from intestinal contents or carcasses. Seven isolates were obtained, of which five and two strains were serotypes 1/2a and 1/2b, respectively. Genetic analysis suggested that there was persistent inhabitation of the pathogen around the area investigated in this study.

Genetic subtyping of <i>Listeria monocytogenes</i> via multiple-locus sequence typing using <i>iap, sigB</i> and <i>actA</i>

“…Bacterial strains : In this study, we used 67 L. monocytogenes strains [15, 31, 34, 35]. These strains were isolated from skin of beef cattle from a Japanese farm (five strains), Japanese patients with listeriosis (seven strains) and meat produced in Japan (37 strains) or imported to Japan from other countries (18 strains) (Table 1…”

“…RFLP analysis : Genomic DNA from L. monocytogenes was extracted and purified as previously described [24, 25, 31, 33]. For RFLP analysis, genomic DNA was digested with restriction enzymes Xba I, Cla I ( Ban III) or Pst I according to the manufacturer’s instructions (Takara Bio, Otsu, Japan).…”

“…Consistent with lineage, we have shown that L. monocytogenes isolated in Japan can be classified roughly into three groups using the iap sequence [34, 35]. We proposed that phylogenetic analysis combined with iap sequencing and whole genome RFLP ( iap -RFLP) is a useful method to genetically differentiate among L. monocytogenes isolates [15, 24, 25, 31, 33]. This method revealed that domestic meat is contaminated by strains of epidemic clone 1 that has been associated with several widespread outbreaks in Europe and the United States, though the frequency of isolation seems to be low [15].…”

Effectiveness of barrier film with a cellulose coating that carries nisin blends for the inhibition of listeria monocytogenes

“…3,4 The occurrence of L. monocytogenes in fresh beef was also established by several studies. [5][6][7] L. monocytogenes is a ubiquitous organism, able to survive and multiply at refrigeration temperatures, in up to 12-13% salt, while certain strains may grow in water activity down to 0.9 and a pH value down…”

Prevalence and Characterization of Foodborne Pathogens in Dairy Cattle in the Eastern Part of Japan

“…The result suggests that the within-farm prevalence of L. monocytogenes is very low and that samples of more than 10 animals are needed to determine the presence of L. monocytogenes on a given dairy farm. Takahashi et al [22] reported that L. monocytogenes was isolated from the skins of three (5%) of 60 beef cattle sampled at an abattoir, although L. monocytogenes isolates were not obtained from large intestinal contents. Ochiai et al [17] reported that L. monocytogenes was isolated from 15.5% (17/110) of retailed beef samples and that the serovars of the isolates were 1/2a (three isolates), 1/2b (two isolates), 1/2c (eight isolates) and 4b (five isolates).…”