Isolation of lipids from plasma by affinity chromatography

Deutsch, Dale G.; Fogleman, David; Kaulla, K. N. von

doi:10.1016/0006-291x(73)91309-0

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1974

1981

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Cited by 21 publications

(2 citation statements)

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“…Dodecylamine-gel was prepared exactly as described by Deutsch et al (1973). The attachment of dodecylamine onto the Sepharose 4B beads was qualitatively demonstrated by reacting the beads with 2,4,6-tri-nitrobenzenesulfonate to produce a stable orange colour (Cuatrecasas and Anfinsen, 1971).…”

Section: Methodsmentioning

confidence: 99%

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Removal of non-specific serum inhibitors of haemagglutination of rubella virus by treatment with dodecylamine-gel.

Ho¹,

Shortridge²

1976

J Clin Pathol

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sYNoPsis The suitability of using dodecylamine-gel for removing the serum non-antibody-like inhibitors of haemagglutination by rubella virus was studied. Compared with kaolin and MnCl2/ heparin treatment this new procedure appears to have a higher specificity since it removes the non-antibody-like inhibitors from serum without affecting the immunoglobulin level significantly. The potential application of this procedure in routine serological analysis for rubella virus infection is discussed.

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Section: Methodsmentioning

confidence: 99%

“…Recently, Deutsch et al (1973) reported a technique for removing lipids from serum by affinity chromatography on agarose beads with covalently attached Received for publication 16 March 1976 dodecylamine arms. In the light of their success we explored the potential application of this procedure to remove the non-antibody-like inhibitors from serum.…”

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confidence: 99%