Isolation of Legionella Species from Noyu （Unattended Natural Hot Springs in Mountains and Fields） Samples in Japan

Abstract

In order to understand the habitation conditions of the bacteria of the genus Legionella in Noyu (unattended natural hot springs in mountains and fields) in Japan, isolation of Legionella spp. was attempted in the Noyu samples from 11 prefectures nationwide between May and September 2012, and the following results were obtained. Overall, Legionella spp. was isolated from 16 of 43 samples (37.2%). The species was isolated from the Hokkaido region to the Chugoku region but not from the Shikoku region to the Kyushu region. The number of bacteria detected was usually small, less than 5.0 × 10(1) CFU/100 ml, as found in 11 samples (68.8%), while counts of 10(2) or more to 10(3) or less CFU/100 ml were found in two samples (12.5%). Legionella pneumophila was the most commonly found strain, with 19 strains (90.5%) found, and was the dominant species. Regarding the serogrouping, four strains (21.1%) fell under group 1, the most common grouping, followed by three strains (15.8%) in group 3, two strains (10.5%) in group 5, etc. Moreover, the detected bacterial strains other than L. pneumophila included two strains (9.5%) of L. londiniensis. The temperature of the Noyu from which Legionella spp. was isolated was between 33.1°C and 41.5°C with a pH ranging from 5.2 to 8.1. The present report is the first report to clarify the habitation conditions of strains of Legionella spp. isolated from Noyu in Japan.

Pulsed-Field Gel Electrophoresis (PFGE) Pattern Analysis and Chlorine-Resistance of Legionella pneumophila Isolated from Hot Spring Water Samples

“…We have previously isolated L. pneumophila strains at a high frequency from the circulation-type hot spring bath water Furuhata et al, 2011a and Noyu unattended natural hot springs samples Furuhata, et al, 2013a in various parts of Japan. In the present study, we investigated the genotypes of these strains, employing a PFGE pattern analysis.…”

“…Sodium hypochlorite dilutions were added to 200 ml of sterile PBS buffer pH 7.3 to prepare experimental solutions of 0.3-0.8 mg/l as available chlorine. The concentration of free residual chlorine was londiniensis Furuhata, et al, 2013a . I n t h i s s t u d y, w e u s e d a p u l s e d -f i e l d g e l electrophoresis pattern analysis to genotype L. pneumophila isolated from both unchlorinated Noyu unattended natural hot springs and chlorinated hot spring bath water samples collected throughout Japan, and investigated the relationship between the chlorine resistance of the isolates and the clustering groups.…”

Investigations on Contamination of Environmental Water Samples by <i>Legionella</i> using Real-Time Quantitative PCR Combined with Amoebic Co-Culturing

“…The culture method is routinely used to test Legionella spp. in environmental samples (Totaro et al, 2017;Furuhata et al, 2013) . However, this method has certain limitations associated with the culture characteristics of Legionella spp.…”

Roseomonas elaeocarpi sp. nov., isolated from olive (Elaeocarpus hygrophilus Kurz.) phyllosphere

“…On: Sat, 12 May 2018 03:08:17 major fatty acid of this genus was for C 18 : 1 v7c under the conditions studied (Gallego et al, 2006;Yoon et al, 2007;Furuhata et al, 2008Furuhata et al, , 2013Yoo et al, 2008;Zhang et al, 2008;Venkata Ramana et al, 2010;Lopes et al, 2011;Kim et al, 2013;Nutaratat et al, 2013;Tonouchi & Tazawa, 2014;Chen et al, 2014;Dong et al, 2014;He et al, 2014, Kim & Ka, 2014 except Roseomonas lacus TH-G33 T , which had iso-C 16 : 0 as the predominant fatty acid (Jiang et al, 2006). Cells contained ubiquinone 10 (Q-10) as the major quinone (Kim et al, 2013;Dong et al, 2014;Chen et al, 2014).…”

“…Based on 16S rRNA gene sequence analysis, this genus belongs to the Alphaproteobacteria (Weyant & Whitney, 2005). At the time of writing, 23 species and two subspecies with validly published names are members of the genus Roseomonas, recently recognized species including Roseomonas aerophila (Kim et al, 2013), 'Roseomonas musae' (Nutaratat et al, 2013), Roseomonas tokyonensis (Furuhata et al, 2013), 'Roseomonas aceris' (Tonouchi & Tazawa, 2014), Roseomonas alkaliterrae (Dong et al, 2014), Roseomonas rhizosphaerae (Chen et al, 2014), 'Roseomonas sediminicola' (He et al, 2014) and Roseomonas soli (Kim & Ka, 2014). There have been reports on the isolation of Roseomonas species from various sources such as medical and environmental samples including plant phyllosphere.…”