Isolation of lactic acid bacteria exhibiting high scavenging activity for environmental hydrogen peroxide from fermented foods and its two scavenging enzymes for hydrogen peroxide

Watanabe, Akio; Kaneko, Chiaki; Hamada, Yoshiro; Takeda, Kouji; Kimata, Shinya; Matsumoto, Takashi; Abe, Akira; Tanaka, Naoto; Okada, Sanae; Uchino, Makoto; Satoh, Jun‐ichi; Nakagawa, Junichi; Niimura, Youichi

doi:10.2323/jgam.62.75

Cited by 9 publications

(13 citation statements)

References 23 publications

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“…To screen bacteria exhibiting high lipid hydroperoxide-eliminating activity, we used modified GYP medium [9] supplemented with 1% linoleic acid hydroperoxide serving as the fatty acid hydroperoxide. Linoleic acid hydroperoxide for medium was prepared in bulk by oxidizing 250 ml of linoleic acid though the incorporating of 100% O 2 at 70˚C.…”

Section: Selective Isolation Medium For Lactic Acid Bacteriamentioning

confidence: 99%

“…Therefore, lactic acid bacteria that can eliminate environmental lipid hydroperoxide directly, should be vigorously investigated as probiotics to prevent bowel diseases. Previously, we isolated the Pediococcus pentosaceus Be1 strain that reduces directly environmental hydrogen peroxide [9]. Based on this previous isolation method, which was improved upon in this study, we successfully isolated the Lactobacillus plantarum P1-2 strain that reduces environmental fatty acid hydroperoxides, which are primary peroxidation products of free fatty acids and are also derived from the hydrolysis of esterified lipid hydroperoxides.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats

et al. 2020

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Reinforcement of the hydroperoxide-eliminating activity in the small and large intestines should prevent associated diseases. We previously isolated a lactic acid bacterium, Pediococcus pentosaceus Be1 that facilitates a 2-electron reduction of hydrogen peroxide to water. In this study, we successfully isolated an alternative lactic acid bacterium, Lactobacillus plantarum P1-2, that can efficiently reduce environmental alkyl hydroperoxides and fatty acid hydroperoxides to their corresponding hydroxyl derivatives through a 2-electron reduction. Each strain exhibited a wide concentration range with regard to the environmental reducing activity for each hydroperoxide. Given this, the two lactic acid bacteria were orally administered to an oxygen-sensitive short-lived nematode mutant, and this resulted in a significant expansion of its lifespan. This observation suggests that P. pentosaceus Be1 and L. plantarum P1-2 inhibit internal oxidative stress. To determine the specific organs involved in this response, we performed a similar experiment in rats, involving induced lipid peroxidation by iron-overloading. We observed that only L. plantarum P1-2 inhibited colonic mucosa lipid peroxidation in rats with induced oxidative stress. Citation: Watanabe A, Yamaguchi T, Murota K, Ishii N, Terao J, Okada S, et al. (2020) Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats. PLoS ONE 15(2): e0215113. https://doi.org/10.

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Section: Selective Isolation Medium For Lactic Acid Bacteriamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats

et al. 2020

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“…Hydroperoxides, such as hydrogen peroxide and lipid hydroperoxides, are toxic and thought to contribute to various diseases and the aging process. Two isolated lactic acid bacteria, P. pentosaceus Be1[8] and Lactobacillus plantarum P1-2, show a 2-electron reduction of hydrogen peroxide to water and of fatty acid hydroperoxides, a type of lipid hydroperoxide, to the corresponding hydroxy derivatives. The oxygen-sensitive and short-lived nematode mutant, C. elegans Δfer-15;mev-1 , accelerates the accumulation of hydroperoxides inside the body due to a lack of superoxide dismutases.…”

Section: Discussionmentioning

confidence: 99%

“…To screen bacteria exhibiting high lipid hydroperoxide-eliminating activity, we used modified GYP medium [8] supplemented with 1% linoleic acid hydroperoxide serving as the fatty acid hydroperoxide. Linoleic acid hydroperoxide was prepared in bulk by oxidizing 250 ml of linoleic acid by incorporating 100% O2 at 70°C.…”

Section: Methodsmentioning

confidence: 99%

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Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats

Watanabe

Yamaguchi

Murota

et al. 2019

Preprint

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Reinforcement of hydroperoxide-eliminating activity in the intestines and colon should prevent associated diseases. We previously isolated a lactic acid bacterium,Pediococcus pentosaceusBe1, that facilitates a 2-electron reduction of hydrogen peroxide to water. In this study, we successfully isolated an alternative lactic acid bacterium,Lactobacillus plantarumP1-2, that can efficiently reduce environmental alkyl hydroperoxides and fatty acid hydroperoxides to their corresponding hydroxy derivatives through a 2-electron reduction. Each strain exhibited a wide concentration range with regard to the environmental reducing activity for each hydroperoxide. Given this, the two lactic acid bacteria were orally administered to the oxygen-sensitive short-lived nematode mutant, and this resulted in a significant expansion of its lifespan. This observation suggests thatP. pentosaceusBe1 andL. plantarumP1-2 inhibit internal oxidative stress. To determine the specific organs involved in this response, we performed a similar experiment in rats, involving induced lipid peroxidation by iron-overloading. We observed that onlyL. plantarumP1-2 inhibited colonic mucosa lipid peroxidation in rats with induced oxidative stress.

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Pediococcus pentosaceus PP34 Ameliorates 5-Fluorouracil-Induced Intestinal Mucositis via Inhibiting Oxidative Stress and Restoring the Gut Microbiota

He,

Li,

Zhang

et al. 2024

Probiotics & Antimicro. Prot.

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Isolation of lactic acid bacteria exhibiting high scavenging activity for environmental hydrogen peroxide from fermented foods and its two scavenging enzymes for hydrogen peroxide

Cited by 9 publications

References 23 publications

Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats

Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats

Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats

Pediococcus pentosaceus PP34 Ameliorates 5-Fluorouracil-Induced Intestinal Mucositis via Inhibiting Oxidative Stress and Restoring the Gut Microbiota

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