Isolation of inulin‐type oligosaccharides from Chinese traditional medicine: Morinda officinalis How and their characterization using ESI‐MS/MS

Abstract

Inulin-type oligosaccharides with different DP were prepared by size-exclusion chromatography and purity of each oligosaccharide was determined by HPLC equipped with cyclodextrin-bond column. The purities of obtained inulin-type oligosaccharides with different DP were more than 98% by one-step process. The DP and molecular weight were obtained through ESI-MS in negative mode. The characterization of the inulin-type oligosaccharides with different DP was studied by MS/MS spectra obtained by collision-induced dissociation of molecular ions ([M-H](-)). When the DP was lower, the fragment ions were formed through cross-ring cleavages of two bonds within the sugar ring and glycosidic cleavages. However, with the increase of DP, the ions resulting from glycosidic cleavages between two sugar residues were predominant.

Extraction, degree of polymerization determination and prebiotic effect evaluation of inulin from Jerusalem artichoke

“…After that, percent of inulin begins to decrease gradually and reached 7.3% at the last time point. Compared with previous reports (Frese, Dambroth, & Bramm, 1991;Liu et al, 2014;Yang et al, 2010), the yield of inulin extracted from Jerusalem artichoke was greater than or equal to the yield in other species (i.e., chicory, burdock, Morinda officinalis). This indicates that Jerusalem artichoke could be a resource to produce inulin in China.…”

From Small to Big Molecules: How Do We Prevent and Delay the Progression of Age-Related Neurodegeneration?

“…MP-1 attenuated the overload of intracellular calcium ion and down-regulated the expression of mRNA for nerve growth factor (NGF) [126,127]. Chemical analysis reveals that MP-1 is an insulin-type fructan with simple linear (2 1)-linked structure, and that its glucose/fructose ratio is 1:21 [128].…”

Preparation of inulin-type fructooligosaccharides using fast protein liquid chromatography coupled with refractive index detection

“…Molecular mass of individual purified FOS was determined in negative ion mode mass spectrometry [13,31]. Their MS/MS spectra were shown in Fig.…”

“…Endl) and burdock (Arctium lappa L.), which are usually used for producing FOS in food industry [10][11][12]. Actually, FOS are also abundant in some traditional Chinese medicines including Morinda Officinalis How, Rehmannia glutinosa Libosch and Polygala tenuifolia Willd [13,14]. In this study, burdock, a famous healthy and nutritive food in China, Korea and Japan, was chosen as a sample for preparation and purification of FOS.…”