Isolation of inhibin from bovine follicular fluid

Robertson, David; Foulds, Lynda M.; Leversha, Lorraine; Morgan, Francis J.; Hearn, Milton T. W.; Burger, Henry G.; Wettenhall, Richard E.H.; Kretser, David M de

doi:10.1016/0006-291x(85)90594-7

Cited by 494 publications

(142 citation statements)

References 17 publications

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“…These results agree with the results obtained when either highly purified bovine or porcine inhibin was tested in rat pituitary cell culture (Robertson et al, 1985;Ling et al, 1987;Miyamoto et al, 1987) or injected in ovariectomized heifers (Ireland et al, 1983;Beard et al, 1990 (Nakamura et al, 1990; Kogawa et al, 1991;Shimonaka et al, 1991 Therefore, the purification of bovine inhibin with H256 as ligand may also copurify follistatin and activin,, and these bound proteins appear as a multiplicity of bands in the 25-35-kDa region. These results underline the potential problems of purifying inhibin by immunoaffinity chromatography from biological fluid.…”

Section: Dispersed Bovine Pituitary Cells In Culturesupporting

confidence: 86%

“…Nous avons éta-bli un système de culture de cellules hypophysaires bovines dispersées en culture afin de démontrer les effets du liquide folliculaire traité au charbon (BFF) ou de l'inhibine bovine partiellement purifiée par chromatographie d'immuno-affinité (IPI) sur la relâche spontanée de l'hormone folliculostimulante (FSH) et lutéinisante (LH). Ces dernières ont été mises en culture à des concentrations de 0,25, 0,5 ou 1 x 10 6 celluleslpuit (clp) et incubées pour 48 h. Le milieu de culture fut remplacé et additionné de BFF (0,0,54,2,7,13,7,68,7 (Padmanabhan et al, 1984;Robertson et al, 1985Robertson et al, , 1986Robertson et al, , 1987. Inhibin has been identified as one of the proteins responsible for suppression of spontaneous FSH release (Ying, 1988;Ling et al, 1990;Knight, 1991).…”

Section: Fsh I Lh I Inhibin I Follicular Fluid I Pituitary I Bovineunclassified

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Effects of bovine follicular fluid and partially purified bovine inhibin on FSH and LH release by bovine pituitary cells in culture

Lussier¹,

Carruthers²,

Murphy³

1993

Reprod. Nutr. Dev.

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Summary ― We have established a dispersed bovine pituitary cell culture system to study the effects of charcoal-extracted bovine follicular fluid (BFF) or bovine inhibin, partially purified by immunoaffinity chromatography (IPI), on the spontaneous release of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Pituitary cells were plated at 0.25, 0.5 or 1 x 10 6 viable cells/well (c/w) and incubated for 48 h. The medium was replaced and BFF (0, 0.54, 2.7, 13.7, 68.7 (0, 0,54, 2,7, 13,7, 68,7

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Section: Dispersed Bovine Pituitary Cells In Culturesupporting

confidence: 86%

Section: Fsh I Lh I Inhibin I Follicular Fluid I Pituitary I Bovineunclassified

Effects of bovine follicular fluid and partially purified bovine inhibin on FSH and LH release by bovine pituitary cells in culture

Lussier¹,

Carruthers²,

Murphy³

1993

Reprod. Nutr. Dev.

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“…Activin stimulates FSH whereas inhibin and follistatin are inhibitory (Ling et al, 1985;Robertson et al, 1985;Ling et al, 1986;Vale et al, 1986;Robertson et al, 1987;Ueno et al, 1987). The inhibins and activins, members of the transforming growth factor ␤ (TGF-␤) superfamily, are known to play pivotal roles in developmental and reproductive processes.…”

mentioning

confidence: 99%

Regulation of ovarian function by the TGF-beta superfamily and follistatin

et al. 2003

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The role of follistatin as an activin-binding protein has dominated the study of this molecule for the last 10 years. However, there is emerging evidence that follistatin has a role in modulating the biology of other members of the transforming growth factor beta (TGF-beta) superfamily. This review summarizes the current concepts encompassing follistatin biochemistry as well as molecules with which it is functionally associated. Moreover, the importance of the two follistatin isoforms (follistatin-288 and follistatin-315) is discussed with particular emphasis on the regulation of the ovary. In addition to activin, this review discusses the functions of other members of the TGF-beta superfamily, for example growth differentiation factor 9 (GDF-9), bone morphogenetic protein 15 (BMP-15), BMP-6, BMP-4 and BMP-7, in the ovary, and the potential interactions between follistatin and these growth factors. The complex network of TGF-beta superfamily growth factor members involved in the modulation of ovarian function and the interactions of follistatin with these proteins is highlighted.

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“…A main task of ovary-derived activins and inhibins in the adult vertebrate is the regulation of availability of folliclestimulating hormone (FSH), a key endocrine regulator of ovarian function (12)(13)(14)(15). FSH is a glycoprotein secreted from the anterior pituitary gland, acting exclusively on ovarian granulosa cells and testicular Sertoli cells (16).…”

mentioning

confidence: 99%

Activin A Signaling Induces Smad2, but Not Smad3, Requiring Protein Kinase A Activity in Granulosa Cells from the Avian Ovary

Schmierer

Schuster

Shkumatava

et al. 2003

Journal of Biological Chemistry

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Activin A signaling is an important regulator of ovarian granulosa cell function. The cytosolic signal transducer Smad2 is most highly expressed in chicken granulosa cells (cGC) of preovulatory follicles. Moreover, Smad2 shows predominant nuclear localization in freshly isolated cGC, indicating active Smad signaling in vivo. Primary cGC cultured in vitro require activin A to sustain high Smad2 levels, which otherwise drop dramatically in the absence of activin A. This activin A-dependent Smad2 expression is abrogated by protein kinase A (PKA) inhibitors, suggesting a role for PKA in activin signaling. In the absence of activin A, strong PKA activators such as follicle-stimulating hormone (FSH) and 8-bromo-cyclic AMP fail to elicit Smad2 induction. However, FSH and 8-bromo-cyclic AMP boost activin A-dependent Smad2 up-regulation, giving rise to Smad2 levels similar to expression in vivo levels. Interestingly, the effect is specific for Smad2, since expression of the structurally and functionally closely related Smad3 remains entirely unaffected. Hence, activin A induces Smad2, but not Smad3, to high levels requiring PKA activation. Since Smad2 and Smad3 target distinct yet overlapping sets of TGF-␤/activin-responsive genes, the selective Smad2 induction by FSH/activin A could allow FSH to efficiently modulate the transcriptional readout of activin A signaling in avian granulosa cells.

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Isolation of inhibin from bovine follicular fluid

Cited by 494 publications

References 17 publications

Effects of bovine follicular fluid and partially purified bovine inhibin on FSH and LH release by bovine pituitary cells in culture

Effects of bovine follicular fluid and partially purified bovine inhibin on FSH and LH release by bovine pituitary cells in culture

Regulation of ovarian function by the TGF-beta superfamily and follistatin

Activin A Signaling Induces Smad2, but Not Smad3, Requiring Protein Kinase A Activity in Granulosa Cells from the Avian Ovary

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