Isolation of infectious pancreatic necrosis (IPN) virus from non‐salmonid fish

Adair, B. M.; Ferguson, H. W.

doi:10.1111/j.1365-2761.1981.tb01110.x

Cited by 26 publications

(13 citation statements)

References 11 publications

(15 reference statements)

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“…Cells were grown in MEM with Earle's salts (Flow Laboratories) supplemented with 10 ~ foetal calf serum (MEM-10), penicillin (100 international units/ml), streptomycin (100 ~tg/ml), gentamicin (25 ~tg/ml) and fungizone (0-25 ~tg/ml). Procedures for virus isolation were similar to those described by Adair & Ferguson (1981). Tissue samples from different organs of diseased rainbow trout or eel were homogenized with nine volumes of MEM containing double strength antibiotics.…”

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confidence: 99%

Comparison of RNAs and Polypeptides of Infectious Pancreatic Necrosis Virus Isolates from Eel and Rainbow Trout

Hsu

Chen

1989

Journal of General Virology

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SUMMARYThirteen isolates of infectious pancreatic necrosis virus (IPNV) from eel and rainbow trout in Taiwan were compared with the selected serotypes AB, SP and VR-299 by PAGE analysis of their RNA genomes and early polypeptides. All the IPNV isolates from eels (1984 to 1986) from Lu Kang and Ping Tung, and from rainbow trout eggs and fry from Dan Sway (1985 to 1986) were most closely related to the AB serotype. However four rainbow trout isolates from Dan Sway (1984) had a similar RNA pattern to that of VR-299, whereas their early polypeptides were different, showing evidence of some mutations. Both RNA and polypeptide PAGE patterns were used to distinguish the isolates from known selected IPNV strains showing that this approach can be used for epizootiological studies.

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confidence: 99%

Comparison of RNAs and Polypeptides of Infectious Pancreatic Necrosis Virus Isolates from Eel and Rainbow Trout

Hsu

Chen

1989

Journal of General Virology

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“…The isolated virus was not pathogenic to the original host nor to rainbow trout fry. The IPNV-Ab-subtype has been isolated from rainbow trout UBRGENSEN, 1971); from goldfish, discus fish and bream (ADAIR and FERGUSON, 1981); from southern flounder (MCALLISTER et al, 1983); from Japanese eel and tilapia . The IPNV-Ab-subtyp usually has a low virulence.…”

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The existence of three different viral agents in a tumour bearing European eel (Anguilla anguilla)

Ahne¹,

Thomsen²

1985

Zentralblatt für Veterinärmedizin Reihe B

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Summary Three different viral agents were isolated from a tumour bearing eel. Two of the agents were identified as IPNV‐subtype Ab and as Rhabdovirus anguilla subtype EVX. The third syncytia‐forming agent has not as yet been classified. Zusammenfassung Das Vorkommen 3 verschiedener Viren bei einem Aal mit Stomatopapillom Von einem tumortragenden Aal konnten 3 verschiedene Viren isoliert werden. 2 der Erreger wurden als IPNV‐Subtyp Ab und als Rhabdovirus anguilla‐Subtyp EVX identifiziert. Das 3.syncytienbildende Agens konnte bisher noch nicht klassifiziert werden. Résumé Présence de trois virus différents dans un stomatopapillome chez une anguille Trois virus différents ont pu être isolés chez une anguille atteinte d'une tumeur. Deux virus ont été identifiés comme IPNV — sous‐type Ab et comme Rhabdovirus anguilla, sous‐type EVX. Le troisième agent, syncytia‐formateur, n'a pas pu être encore classé. Resumen La existencia de tres agentes virales diferentes en una anguila europea (Anguilla anguilla) con estomatopapiloma De una anguila, que era portadora de un tumor, se pudieron aislar 3 virus diferentes. 2 de estos agentes fueron identificados como IPNV‐subtipo Ab y como Rhabdovirus anguilla — subtipo EVX. El tercer agente, sinciciógeno, no pudo ser clasificado hasta la fecha.

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“…Two of these serotypes, AB and SP (Jorgensen & Kehlet, 1971), are found only in Europe. Both the AB and SP serotypes have been detected in trout, in several nonsalmonid species and in elvers in Europe (Hudson et al, 1981 ;Adair & Ferguson, 1981 ;Castric & Chastel, 1980; Munro et al, 1976; Ahne, 1980). The third serotype of IPNV is prevalent in North America and is represented by reference strain VR-299 (Wolf & Quimby, 1971).…”

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Biochemical Characterization of Eel Virus European

Hedrick

Okamoto

Sano

et al. 1983

Journal of General Virology

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SUMMARYThe polypeptide and RNA compositions of purified virions of eel virus European were compared to those of three strains (VR-299, SP and AB) of infectious pancreatic necrosis virus (IPNV) isolated from trout. All three IPNV strains could be distinguished by the relative mobilities of either the virion polypeptides or the doublestranded RNA genome segments. The eel virus had a similar polypeptide profile to strain AB IPNV but differences between the two viruses in the migration of genome segments indicated each was unique. Annual epizootics among pond-cultured Japanese eels (Anguilla japonica) in Shizuoka prefecture, Japan have been reported since 1969 (Egusa, 1970). Environmental and physiological factors alone or in combination with a bacterium of the genus Cytophaga were believed to be the major causes of the observed epizootics (Egusa et al., 1971 ;Egusa, 1976). Virological examinations of diseased eels, however, revealed the presence of a previously undescribed virus (Sano, 1976). Examinations of eels from farms in 10 different locations indicated that this same virus was present in several populations of cultured eels in Japan (Sano et al., 1981). This disease outbreak followed the importation of European eels (Anguilla anguilla) to Shizuoka prefecture. The virus was also isolated from these imported eels. Because the virus showed an antigenic relationship to a European strain of infectious pancreatic necrosis virus (IPNV), it was designated eel virus European (EVE).Infectious pancreatic necrosis virus is a known pathogen of hatchery reared brook (Salvelinus fontinalis) and rainbow (Salmo gairdneri) trout and is found in many countries where these fish are cultured (Wolf, 1966). Three major serological groups or serotypes of IPNV have been identified (Okamoto et al., 1983;Macdonald & Gower, 1981). Two of these serotypes, AB and SP (Jorgensen & Kehlet, 1971), are found only in Europe. Both the AB and SP serotypes have been detected in trout, in several nonsalmonid species and in elvers in Europe (Hudson et al., 1981 ;Adair & Ferguson, 1981 ;Castric & Chastel, 1980; Munro et al., 1976; Ahne, 1980). The third serotype of IPNV is prevalent in North America and is represented by reference strain VR-299 (Wolf & Quimby, 1971).Eel virus European (EVE) is similar to IPNV in particle size and morphology, cytopathogenic effect in cell culture and buoyant density in caesium chloride (Nishimura et al., 1981). Unlike the VR-299 and SP strains, EVE was not pathogenic for rainbow trout even though the virus was found to replicate to high concentrations in several internal organs (Sano et aL, 1981). Similar results were obtained with the AB strain of IPNV which was less virulent for rainbow trout (Jorgensen & Kehlet, 1971) than SP or VR-299 IPNV. Cross-neutralization studies have shown EVE to be closely related to the AB strain of IPNV (Okamoto et al., 1983). The results presented here provide the first comparisons of the RNA and polypeptides of VR-299, SP and AB IPNV with EVE. From this study, we have confirmed that E...

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Isolation of infectious pancreatic necrosis (IPN) virus from non‐salmonid fish

Cited by 26 publications

References 11 publications

Comparison of RNAs and Polypeptides of Infectious Pancreatic Necrosis Virus Isolates from Eel and Rainbow Trout

Comparison of RNAs and Polypeptides of Infectious Pancreatic Necrosis Virus Isolates from Eel and Rainbow Trout

The existence of three different viral agents in a tumour bearing European eel (Anguilla anguilla)

Biochemical Characterization of Eel Virus European

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