Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (<i>Rosa canina</i> L.)

Saaby, Lasse; Jäger, Anna K.; Moesby, Lise; Hansen, Erik W.; Christensen, Søren Brøgger

doi:10.1002/ptr.3241

Cited by 65 publications

(42 citation statements)

References 28 publications

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“…Rosa canina fruits (rose hip) were reported to contain antioxidant nutrients and an anti-inflammatory galactolipid (Kirkeskov et al, 2011). DCM extracts from rose hip powder and the triterpene acids oleanolic acid and ursolic acid (IC 50 21±6 μM) isolated thereof significantly and concentration dependently inhibited LPS-induced IL-6 release from Mono Mac 6 cells (Saaby et al, 2011). Rose hip powder extracts of different polarity were tested for inhibition of COX-1, COX-2, and 5-LOX-mediated leukotriene B4 (LTB4) formation as well as for DPPH-radical-scavenging capacity.…”

Section: Resultsmentioning

confidence: 99%

Ethnopharmacological in vitro studies on Austria's folk medicine—An unexplored lore in vitro anti-inflammatory activities of 71 Austrian traditional herbal drugs

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Mihály-Bison

et al. 2013

Journal of Ethnopharmacology

207

130

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Ethnopharmacological relevanceIn Austria, like in most Western countries, knowledge about traditional medicinal plants is becoming scarce. Searching the literature concerning Austria's ethnomedicine reveals its scant scientific exploration.Aiming to substantiate the potential of medicinal plants traditionally used in Austria, 63 plant species or genera with claimed anti-inflammatory properties listed in the VOLKSMED database were assessed for their in vitro anti-inflammatory activity.Material and methods71 herbal drugs from 63 plant species or genera were extracted using solvents of varying polarities and subsequently depleted from the bulk constituents, chlorophylls and tannins to avoid possible interferences with the assays. The obtained 257 extracts were assessed for their in vitro anti-inflammatory activity. The expression of the inflammatory mediators E-selectin and interleukin-8 (IL-8), induced by the inflammatory stimuli tumor necrosis factor alpha (TNF-α) and the bacterial product lipopolysaccharide (LPS) was measured in endothelial cells. The potential of the extracts to activate the nuclear factors PPARα and PPARγ and to inhibit TNF-α-induced activation of the nuclear factor-kappa B (NF-κB) in HEK293 cells was determined by luciferase reporter gene assays.ResultsIn total, extracts from 67 of the 71 assessed herbal drugs revealed anti-inflammatory activity in the applied in vitro test systems. Thereby, 30 could downregulate E-selectin or IL-8 gene expression, 28 were strong activators of PPARα or PPARγ (inducing activation of more than 2-fold at a concentration of 10 µg/mL) and 21 evoked a strong inhibition of NF-κB (inhibition of more than 80% at 10 µg/mL).ConclusionOur research supports the efficacy of herbal drugs reported in Austrian folk medicine used for ailments associated with inflammatory processes. Hence, an ethnopharmacological screening approach is a useful tool for the discovery of new drug leads.

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Section: Resultsmentioning

confidence: 99%

Ethnopharmacological in vitro studies on Austria's folk medicine—An unexplored lore in vitro anti-inflammatory activities of 71 Austrian traditional herbal drugs

Vogl

Picker

Mihály-Bison

et al. 2013

Journal of Ethnopharmacology

207

130

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“…Several natural triterpenes inhibit IKK and block NF-κB activation. In this way, OA and UA reduce tumor necrosis factor-α–induced E-selectin expression in human endothelial cells (57), inhibit IL-6 release in lipopolysaccharide-activated Mono Mac 6 cells (58), and suppress the endothelin-1 pathway in Zucker diabetic rats (59). BA also represses NF-κB in human colorectal carcinoma HCT116, colon carcinoma Caco-2, and lung carcinoma H1299 cells, with these effects being correlated with its ability to inhibit IKK (60).…”

Section: Improvement Of the Insulin Responsementioning

confidence: 99%

Biochemical Basis of the Antidiabetic Activity of Oleanolic Acid and Related Pentacyclic Triterpenes

et al. 2013

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Oleanolic acid (OA), a natural component of many plant food and medicinal herbs, is endowed with a wide range of pharmacological properties whose therapeutic potential has only partly been exploited until now. Throughout complex and multifactorial mechanisms, OA exerts beneficial effects against diabetes and metabolic syndrome. It improves insulin response, preserves functionality and survival of β-cells, and protects against diabetes complications. OA may directly modulate enzymes connected to insulin biosynthesis, secretion, and signaling. However, its major contributions appear to be derived from the interaction with important transduction pathways, and many of its effects are consistently related to activation of the transcription factor Nrf2. Doing that, OA induces the expression of antioxidant enzymes and phase II response genes, blocks NF-κB, and represses the polyol pathway, AGEs production, and hyperlipidemia. The management of type 2 diabetes requires an integrated approach, which includes the early intervention to prevent or delay the disease progression, and the use of therapies to control glycemia and lipidemia in its late stages. In this sense, the use of functional foods or drugs containing OA is, undoubtedly, an interesting path.

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“…OA is also found in soybeans (Zhang and Popovich, 2009), filamentous fungi, and Sedium aizoon (Laszczyk, 2009). Ginseng, Thunder-God-Vine, rose hip powder, and many medicinal herbs contain OA as a natural product (Liu, 1995; Saaby et al, 2011). The concentrations of OA can be as high as 1–3% as in Ginseng, papaya fruit, olive fruit, apple skin, and dark plums (Pollier and Goossens, 2012).…”

Section: Introductionmentioning

confidence: 99%

Oleanolic acid alters bile acid metabolism and produces cholestatic liver injury in mice

Liu

Zhang

et al. 2013

Toxicology and Applied Pharmacology

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Oleanolic acid (OA) is a triterpenoids that exists widely in plants. OA is effective in protecting against hepatotoxicants. Whereas a low dose of OA is hepatoprotective, higher doses and longer-term use of OA produce liver injury. This study characterized OA-induced liver injury in mice. Adult C57BL/6 mice were given OA at doses of 0, 22.5, 45, 90, and 135 mg/kg, s.c., daily for 5 days, and liver injury was observed at doses of 90 mg/kg and above, as evidenced by increases in serum activities of alanine aminotransferase and alkaline phosphatase, increases in serum total bilirubin, as well as by liver histopathology. OA-induced cholestatic liver injury was further evidenced by marked increases of both unconjugated and conjugated bile acids (BAs) in serum. Gene and protein expression analysis suggested that livers of OA-treated mice had adaptive responses to prevent BA accumulation by suppressing BA biosynthetic enzyme genes (Cyp7a1, 8b1, 27a1, and 7b1); lowering BA uptake transporters (Ntcp and Oatp1b2); and increasing a BA efflux transporter (Ostβ). OA increased the expression of Nrf2 and its target gene, Nqo1, but decreased the expression of AhR, CAR and PPARα along with their target genes, Cyp1a2, Cyp2b10 and Cyp4a10. OA had minimal effects on PXR and Cyp3a11. Taken together, the present study characterized OA-induced liver injury, which is associated with altered BA homeostasis, and alerts its toxicity potential.

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Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa canina L.)

Cited by 65 publications

References 28 publications

Ethnopharmacological in vitro studies on Austria's folk medicine—An unexplored lore in vitro anti-inflammatory activities of 71 Austrian traditional herbal drugs

Ethnopharmacological in vitro studies on Austria's folk medicine—An unexplored lore in vitro anti-inflammatory activities of 71 Austrian traditional herbal drugs

Biochemical Basis of the Antidiabetic Activity of Oleanolic Acid and Related Pentacyclic Triterpenes

Oleanolic acid alters bile acid metabolism and produces cholestatic liver injury in mice

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