Isolation of IgG Associated with Human Placenta

Ignat’eva, N. V.; Зиганшина, М. М.; Шилова, Н. В.; Khasbiullina, Nailya; Bovin, Nicolai V.; Tyutyunnik, Tyutyunnik V.L.; Сухих, Г. Т.

doi:10.1007/s10517-019-04474-4

Cited by 4 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As a central factor in immune mediation, the elevated levels of secretory IgA in the colon help to prevent intestinal damage and restore mucosal barrier function ( 53 ). IgG exerts important protective and regulatory effects in the placental barrier ( 54 ) and IgM serves an important role in its own immune regulation ( 55 ). In the present study, GI increased the levels of Ig in the UC mice to stimulate an immune response.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of Gloeostereum�incarnatum on ulcerative colitis via modulation of Nrf2/NF‑κB signaling in C57BL/6 mice

Liu

Zhang

et al. 2020

Mol Med Rep

View full text Add to dashboard Cite

Chronic non-specific inflammatory cell infiltration of the colon is generally considered to be the cause of ulcerative colitis (UC). Gloeostereum incarnatum (GI), a fungus rich in amino acids and fatty acids, exhibits a variety of biological functions. In the present study, GI was identified to contain 15 fatty acids, 17 amino acids and 11 metallic elements. The protective effect of GI against UC was investigated in C57BL/6 mice with UC induced by free drinking 3.5% dextran sulfate sodium (DSS). After a 21-day oral administration, GI prevented weight loss, enhancement of the disease activity index and colonic pathological alterations in mice with UC. GI reduced the levels of pro-inflammatory factors including interleukin (IL)-1β, IL-2, IL-6 and IL-12, tumor necrosis factor α and -β, interferon α and -γ, and pro-oxidative factors including reactive oxygen species and nitric oxide. In addition, it enhanced the levels of immunological factors including immunoglobulin (Ig)A, IgM and IgG, and antioxidative factors including superoxide dismutase and catalase in the serum and/or colon tissues. GI enhanced the expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins and suppressed the phosphorylation of NF-κB signaling in colon tissues. Together, GI was shown to alleviate the physiological and pathological state of DSS-induced UC in mice via its antioxidant and anti-inflammatory functions, which may be associated with its modulation of the activation of Nrf2/NF-κB signaling.

show abstract

Section: Discussionmentioning

confidence: 99%

Protective effect of Gloeostereum�incarnatum on ulcerative colitis via modulation of Nrf2/NF‑κB signaling in C57BL/6 mice

Liu

Zhang

et al. 2020

Mol Med Rep

View full text Add to dashboard Cite

show abstract

“…The plAbs profiles were analysed using a PGA. Previously, it was shown that antibodies eluted from the placenta belong only to IgG class 27 . In our study, both M and G antibodies were investigated, but the signal level of M class antibodies appeared below the selected cut‐off (465 RFU).…”

Section: Resultsmentioning

confidence: 75%

“…Previously, it was shown that antibodies eluted from the placenta belong only to IgG class. 27 In our study, both M and G antibodies were investigated, but the signal level of M class antibodies appeared below the selected cut-off (465 RFU). This finding was further confirmed turbidimetrically (data not shown), and therefore, finally the placental eluates were considered to be free from plAbs (IgM).…”

Section: Glycan-binding Profiles Of Antibodies Eluted From Placenta A...mentioning

confidence: 85%

See 1 more Smart Citation

Repertoire of glycan‐binding placenta‐associated antibodies in healthy pregnancy and in pre‐eclampsia

et al. 2022

View full text Add to dashboard Cite

A possible mechanism of the immune tolerance in pregnancy is production of blocking antibodies which reside in placenta and protect foetal allogeneic cells from the mother's immune system. Their epitope specificity, as well as the nature of the biomolecules masked by them, is unknown. For better understanding of this phenomenon, we attempted to characterize the specificity of antibodies isolated from placentas of women with healthy pregnancy and pre‐eclampsia (PE). It was found that: (1) the repertoire of placental antibodies is significantly less variable and qualitatively different from the peripheral blood; (2) with PE, the repertoire of placental antibodies is narrower than in healthy pregnancy; (3) some antibodies are found almost exclusively in the placenta, and some – only in the placenta of healthy women.

show abstract

“…Kdn-гликаны получены в виде индивидуальных аномеров (95% чистоты по данным ВЭЖХ и ЯМР) [5], конфигурация сиалозидной связи была подтверждена химическими сдвигами и константами спин-спинового взаимодействия, типичными для Н-3 протонов Kdn, как описано в [5]. Сыворотки крови и элюаты из плаценты анализировали с использованием гликочипа как описано в [4] и [8] соответственно. Соответствие образцов и форматов чипа приведено в табл.…”

Section: Kdn-гликаныunclassified

Antibodies Against Unusual Forms of Sialylated Glycans

et al. 2022

View full text Add to dashboard Cite

Previous studies have shown that in the blood of healthy donors (1) there are no natural antibodies against sialylated glycoproteins, which contain Neu5Ac (N-acetylneuraminic acid) as the most widespread form of human sialic acid, and (2) there is a moderate level of antibodies capable of binding unnatural oligosaccharides, where Neu5Ac is beta-linked to a typical mammalian glycan core. In the present study, we investigated antibodies against Neu5Ac in more detail and verified the presence of Kdn (2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid) as a possible cause behind their appearance in humans, taking into account the expected cross-reactivity to Kdn glycans, which are found in bacterial glycoconjugates in both the - and -forms. We observed the binding of peripheral blood immunoglobulins to sialyllactosamines (where sialyl is Kdn or neuraminic acid) in only a very limited number of donors, while the binding to monosaccharide Kdn occurred in all samples, regardless of the configuration of the glycosidic bond of the Kdn moiety. In some individuals, the binding level of some of the immunoglobulins was high. This means that bacterial Kdn glycoconjugates are very unlikely to induce antibodies to Neu5Ac glycans in humans. To determine the reason for the presence of these antibodies, we focused on noninfectious pathologies, as well as on a normal state in which a significant change in the immune system occurs: namely, pregnancy. As a result, we found that 2/3 of pregnant women have IgM in the blood against Neu5Ac2-3Gal1-4GlcNAc. Moreover, IgG class antibodies against Neu5Ac2-3Gal1-4GlcNAc and Neu5Ac2-6Gal1-4GlcNAc were also detected in eluates from the placenta. Presumably, these antibodies block fetal antigens.

show abstract

Isolation of IgG Associated with Human Placenta

Cited by 4 publications

References 8 publications

Protective effect of Gloeostereum�incarnatum on ulcerative colitis via modulation of Nrf2/NF‑κB signaling in C57BL/6 mice

Protective effect of Gloeostereum�incarnatum on ulcerative colitis via modulation of Nrf2/NF‑κB signaling in C57BL/6 mice

Repertoire of glycan‐binding placenta‐associated antibodies in healthy pregnancy and in pre‐eclampsia

Antibodies Against Unusual Forms of Sialylated Glycans

Contact Info

Product

Resources

About