Isolation of <i>Streptomyces</i> Species from Soil

Kharel, Madan K.; Shepherd, Micah D.; Nybo, S. Eric; Smith, Michael L.; Bosserman, Mary A.; Rohr, Jürgen

doi:10.1002/9780471729259.mc10e04s19

Cited by 20 publications

(25 citation statements)

References 6 publications

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“…Although the temperature used in this modified method was lower than that in Kharel et al (2010), we managed to isolate Streptomyces strains from the soil samples from King George Island. The isolates exhibited morphometric and ultrastructural characteristics consistent with the Streptomyces genus (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…1). A modified method described by Kharel et al (2010) was used to isolate Streptomyces species and kill most of the non-spore-forming microbes. One gram of soil sample was weighed and suspended in 10 ml of sterile distilled water.…”

Section: Strain Isolationmentioning

confidence: 99%

“…The suspension was heated at 40°C for 30 min and serially diluted up to 10 3 . Each dilution was spread (0.1 ml) onto the Actinomycete Isolation Agar (Difco) and incubated at room temperature for 7 days (Kharel et al 2010).…”

Section: Strain Isolationmentioning

confidence: 99%

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Isolation and characterization of Antarctic psychrotrophStreptomycessp. strain INACH3013

et al. 2016

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An actinobacterial strain with antimicrobial activity, INACH3013, was isolated from soil collected from Antarctica. The taxonomic status of the isolate was established using a polyphasic approach. The strain was identified as belonging to the genus Streptomyces based on the scanning electron microscopic observation and partial 16S rRNA gene sequence analysis. The sequence analysis revealed that strain INACH3013 is closely related to Streptomyces fildesensis (99.8%), S. beijiangensis (98.1%) and S. purpureus (97.2%). A phylogenetic tree constructed using the partial 16S rRNA gene sequences of strain INACH3013 and closely related strains revealed that INACH3013 fell into the same subclade as S. fildesensis and S. purpureus. Strain INACH3013 was observed to be psychrotolerant, slightly halotolerant (up to 5% NaCl) and capable of inhibiting the growth of seven Gram-negative and eight Gram-positive foodborne pathogens. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of the strain is KJ624755.

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Section: Discussionmentioning

confidence: 99%

Section: Strain Isolationmentioning

confidence: 99%

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Isolation and characterization of Antarctic psychrotrophStreptomycessp. strain INACH3013

et al. 2016

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“…Streptomyces are Gram-positive bacteria and are the largest representative genus of actinomycetes, which are found mostly in soil 34 . They are industrially useful microorganisms that produce a wide variety of antibiotics, and several studies have been carried to investigate the biological effects of Streptomyces species isolated from soil samples 35,36,37 .…”

Section: Discussionmentioning

confidence: 99%

Insecticidal activity of the metabolites of sand soil bacteria in Northern Senegal

Dahmana¹,

Sambou²,

Raoult³

et al. 2020

Preprint

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BackgroundVector-borne deadly diseases cause more than 700,000 deaths annually. They are transmitted by several vectors, among which the mosquito is the most important. Chemical compounds often have devastating side effects, which has led to the abandonment of the majority of them. Biological control has been performed by using formulations of Bacillus sphaericus and Bacillus thuringiensis, but their intensive use has led to the emergence of resistance. Currently, there is an urgent need to develop new alterative molecules from bacteria to use in mosaics or in rotation with already known insecticides and bacteria for the control of vectors, especially mosquitos. Here, we attempted to identify bacterial species with potential anti-mosquito actions.ResultsAmong 208 strains isolated from dry sandy soil from Senegal, eleven bacterial strains from the Bacillus genus (5) and Actinomycetales order (6) were chosen for the following experiments. Then, we tested their secondary metabolites, which were obtained from the supernatant fraction, and their cell wall and cytoplasmic compounds, which were found in the pellet fraction, in 3rd and early 4th Aedes albopictus larvae instars and compared the larval mortality rate with that obtained by using a commercial product. A total of 4/11 (36.4%) of the isolated species exhibited insecticidal activity. B. nealsonii, which is not a well-known bacterium, had the highest larvicidal effect with 70% larval mortality, which highlighted for the first time. The Streptomyces spp. we isolated seem to be potential new species, and 3/5 (60%) of them exhibited insecticidal activity.ConclusionThe bacterial strains isolated in this study may be used to identify the molecular mechanisms of their insecticidal activity.

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“…Experiment 3 could be completed using a different array of bacterial isolates from public, private, or student-generated (depending on class time) culture collections. The bacterial cultures used in the field test of this module utilized Streptomyces species obtained from outdoor air (Weber et al, unpublished data), but this genus can be isolated from soils by using methods described by Kharel et al ( 12 ).…”

Section: Discussionmentioning

confidence: 99%

Hormones and Antibiotics in Nature: A Laboratory Module Designed to Broaden Undergraduate Perspectives on Typically Human-Centered Topics

Weber

2014

J Microbiol Biol Educ.

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Bringing discovery-based research into undergraduate laboratory courses increases student motivation and learning gains over traditional exercises that merely teach technique or demonstrate well-documented phenomena. Laboratory experiences are further enhanced when they are designed to challenge student perspectives on topics relevant to their lives. To this end, a laboratory module on antibiotics and hormones, which are generally discussed in the context of human health, was developed for students to explore the multifaceted roles of antibiotics and hormones in nature (e.g. interspecies communication) via reading primary scientific literature and performing discovery-based experiments. The main objective of this module was to increase the general biological literacy of students as determined by their ability to connect the Five Core Concepts of Biological Literacy (American Association for the Advancement of Science, Vision and Change in Undergraduate Education: A Call to Action, 2011) to the topics “hormones” and “antibiotics” in pre- and postmodule surveys. After discussing unpublished research findings, cell biology students performed experiments demonstrating that: 1) fungi may promote fern growth via hormone production, 2) novel bacterial isolates in the genus Streptomyces produce antifungal compounds, and 3) subinhibitory antibiotic concentrations may enhance soil bacterial growth. The third finding provided evidence supporting a hypothesis framed in a scientific article that students read and discussed. Student perspectives on premodule surveys focused on roles of hormones and antibiotics in the human body (e.g. development, fighting infection), but their broadened postmodule perspectives encompassed the roles of these molecules in organismal communication and possibly the evolution of multicellularity.

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Isolation of Streptomyces Species from Soil

Cited by 20 publications

References 6 publications

Isolation and characterization of Antarctic psychrotrophStreptomycessp. strain INACH3013

Isolation and characterization of Antarctic psychrotrophStreptomycessp. strain INACH3013

Insecticidal activity of the metabolites of sand soil bacteria in Northern Senegal

Hormones and Antibiotics in Nature: A Laboratory Module Designed to Broaden Undergraduate Perspectives on Typically Human-Centered Topics

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