Isolation of Salmonella from Meat Samples and Characterization by Enterobacterial Repetitive Intergenic Consensus–Polymerase Chain Reaction and Antibiotics Test

Abstract

Salmonella is one of the most important foodborne pathogens associated with severe diseases in animals and humans. Meat samples are considered as one of the main sources of Salmonella infections. Consequently, the survey of Salmonella contamination in meat samples is of outmost importance for the control and prevention of severe diseases. In this study, 250 meat samples were selected for surveys of Salmonella contaminations. Results indicated that 12% (n=30) of samples tested were positive to Salmonella. The genetic characterization of 30 Salmonella was studied by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and 22 of ERIC-PCR types were found with D of 94.8%. In addition, the resistant characterization was also carried out using nine antibiotics test, and nine resistant patterns were observed with D of 88.7%. A good correlation was also observed between ERIC-PCR fingerprinting and resistant patterns in some Salmonella such as SAL 6 and SAL 7.

Off-label use of ceftiofur in one-day chicks triggers a short-term increase of ESBL-producing E. coli in the gut

“…Afterwards, the DNA extracted from confirmed ESBL-producing E . coli was adjusted to 50 ng/ μL using a microvolume spectrometer (Colibri, Titertek Berthold, Germany) and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) was used as genotyping method, as previously described [ 13 ]. Shortly, reactions were performed in 25 μL containing 1 pmol of primer, 200 mM of each dNTP, 3 mM of MgCl 2 , 100 ng of genomic DNA, and 1U of Taq DNA polymerase (Invitrogen, Brazil).…”

Genetic Diversity Profiles of Salmonella typhimurium Isolated from Foods of Animal Origin

“…S. typhimurium (7) isolates were selected and fingerprinted using ERIC-PCR assay as described by Ye et al, (2011). Genotyping of Salmonella isolates was done using the oligonucleotide primers, ERIC-1 (5'-ATG TAA GCT CCT GGG GAT TCA C-3') and ERIC-2 (5'-AAG TAA GTG ACT GGG GTG AGC G-3') primer pair targeting the amplification of conserved ERIC sequences in the chromosomal DNA of Salmonella isolates, leading to amplicon patterns specific for an individual organism (Versalovic et al, 1991).…”

Ecological prevalence, genetic diversity, and multidrug resistance of Salmonella enteritidis recovered from broiler and layer chicken farms

“…Salmonella infections, one of the most common foodborne illnesses, continue to pose a global public health risk ( Ye et al, 2011 ; Eguale et al, 2018 ; El-Saadony et al, 2022 ). In the 1950s, the World Health Organization ( WHO ) and the United Nations Food and Agriculture Organization ( FAO ) identified Salmonella as a serious zoonotic bacterium with financial consequences ( Akinola et al, 2019 ; Marouf et al, 2022 ).…”