Isolation of Legionella oakridgensis from Two Patients with Pleural Effusion Living in the Same Geographical Area

Abstract

Two cases of Legionnaire's disease caused by Legionella oakridgensis were diagnosed at the university hospital in Nantes, France. The two patients' isolates were identified by means of phenotyping and genotyping methods. Epidemiological investigations concluded that the first case was hospital acquired while the second case was considered community acquired.

Molecular typing of the Legionella pneumophila population isolated from several locations in a contaminated water network

“…Band tolerance (maximum tolerance in percent of the curve to match bands) was 1%. Pacheco et al, 1996Pacheco et al, , 1997aPacheco et al, ,b, 1998Osek, 1998 ERIC21 5′-AAG TAA GTG ACT GGG GTG AGC G-3′ Van Belkum et al, 1996;Zietz, 2001 SK2 5′-CGG CGG CGG CGG-3′ Lo Presti et al, 1998Presti et al, , 1999Presti et al, , 2000 Taq Stoffel Buffer (10×) (Applied Biosystems, Foster City, CA) 10 mM 10 mM 10 mM 10 mM MgCl 2 (Applied Biosystems, Foster City, CA) 3 mM 2 mM 2 mM 3 mM AmpliTaq ® DNA Polymerase, Stoffel Fragment (Applied Biosystems, CA) 2 u 2 u 2 u 2 u dNTP (Applied Biosystems, Foster City, CA) 200 µM 100 µM 100 µM 200 µM Primer (Genset Oligo, France) 0.8 µM 0.5 µM 1.0 µM 1.0 µM DNA 10 ng 10 ng 10 ng 10 ng Primer hybridisation temperature 36°C 36°C 25°C 30°C…”

Detection of Legionella DNA by PCR of whole-blood samples in a mouse model

“…In the present study, we evaluated PCR on blood samples by comparing the results of this assay with the results of PCR on BALF samples, culture of blood and BALF samples and urinary antigen detection in a mouse model of Legionella pneumonia. Previous studies have reported the usefulness of PCR as a diagnostic test for Legionella pneumonia using respiratory specimens (Cloud et al , 2000; Hirakata et al , 1996; Jaulhac et al , 1998; Jonas et al , 1995; Lo Presti et al , 2000; Weir et al , 1998), with a sensitivity of ≥90 % (Matsiota-Bernard et al , 1994). However, cough is non-productive in most patients with Legionella pneumonia, and clinical symptoms deteriorate rapidly and severely.…”

“…Recently, enzyme immunoassay (EIA) for the detection of Legionella antigen in urine has been used for diagnosis of Legionella pneumonia, with a reported sensitivity of 63–77 % (Benson et al , 2000; Dominguez et al , 1998; Stout & Yu, 1997). PCR has also been used as a rapid diagnostic method, employing samples of BAL fluid (BALF) (Cloud et al , 2000; Jaulhac et al , 1998; Jonas et al , 1995; Matsiota-Bernard et al , 1994; Weir et al , 1998) or pleural effusion (Breiman & Butler, 1998; Hirakata et al , 1996; Lo Presti et al , 2000). However, PCR detection of L. pneumophila using non-invasive specimens is preferable, particularly by family physicians and non-pulmonologists.…”