Isolation of
            <i>Lactobacillus</i>
            strain from curdled milk and investigation of their antimycotoxinogen activity

Ali, Mounira Kara; Chaouche, Noreddine Kacem

doi:10.1111/jfpp.13841

Cited by 4 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The optimal environment for an esterase to catalyze the hydrolysis of the bonds in a carboxyl ester made of short-chain fatty acids is aqueous solution. Esterases can also catalyze the synthesis and transesterification of esters in water-free or water-restricted media [ 2 – 4 ]. Esterases are used in a number of industrial applications in the manufacture of pharmaceuticals, cosmetics, textile, paper, leather, animal foods, foodstuffs (products of dairy), detergents, and environmental management.…”

Section: Introductionmentioning

confidence: 99%

A scalable overexpression of a thermostable recombinant poly-histidine tag carboxyl esterase under lambda promoter: purification, characterization, and protein modelling

Soliman,

Ali,

Duab

et al. 2023

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background As a white biotechnological trend, esterases are thought to be among the most active enzymes’ classes in biocatalysis and synthesis of industrially importance organic compounds. Esterases are used in many applications such as the manufacture of pharmaceuticals, cosmetics, leather, textile, paper, food, dairy products, detergents, and treatment of some environmental pollutants. Results A poly-histidine moiety was added to the C-terminal end of the Geobacillus sp. gene encoding carboxyl esterase (EstB, ac: KJ735452) to facilitate one-step purification. This recombinant protein was successfully expressed in Escherichia coli (E. coli) under control of Lambda promoter (λ). An open reading frame (ORF) of 1500 bps encoding a polypeptide of 499 amino acid residues and a calculated molecular weight (54.7 kD) was identified as carboxyl-esterase B due to its conserved glycine-X-serine-X-glycine motif (G-X-S-X-G) and its high similarity toward other carboxyl esterases, where the 3-D tertiary structure of EstB was determined based on high homology % (94.8) to Est55. The expression was scaled up using 7-L stirred tank bioreactor, where a maximum yield of enzyme was obtained after 3.5 h with SEA 51.76 U/mg protein. The expressed protein was purified until unity using immobilized metal affinity chromatography (IMAC) charged with cobalt and then characterized. The purified enzyme was most active at pH 8.0 and remarkably stable at pH (8–10). Temperature optimum was recorded at 65 °C, and it kept 70% of its activity after 1-h exposure to 60 °C. The active half-live of enzyme was 25 min at 70 °C and a calculated T melting (Tm) at 70 °C. The determined reaction kinetics Michaelis–Menten constant (Km), maximum velocity rate (Vmax), the turnover number (Kcat), and catalytic efficiency (Kcat/Km) of the pure enzyme were found 22.756 mM, 164.47 U/ml (59.6 min−1), and (2.619 mol/ min), respectively. Conclusion Creation of a recombinant 6 × -His estB derived from a thermophile Geobacillus sp. was performed successfully and then overexpressed under λ-promoter. In a bench scale bioreactor, the overexpression was grown up, followed by one-step purification and biochemical characterization. The recorded promising pH and temperature stability properties suggest that this expressed carboxyl esterase could be used in many industrial sectors.

show abstract

Section: Introductionmentioning

confidence: 99%

A scalable overexpression of a thermostable recombinant poly-histidine tag carboxyl esterase under lambda promoter: purification, characterization, and protein modelling

Soliman,

Ali,

Duab

et al. 2023

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…These bacteria contribute significantly to improve colonization resistance in the gastrointestinal tract by producing different substances (bacteriocins such as lacticin and nisin) useful against various enteric pathogens, such as Bacillus spp., Escherichia coli , Klebsiella spp., Proteus spp., Pseudomonas spp., Shigella spp., Salmonella spp., and Vibrio spp. Such outcomes can be attributed to the probiotic properties of LAB [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

First report on the probiotic potential of <i>Mammaliicoccus sciuri</i> isolated from raw goat milk

Naqqash

WAZIR

Aslam

et al. 2022

Bioscience of Microbiota, Food and Health

View full text Add to dashboard Cite

Probiotics are considered effective microbial dietary supplements that provide beneficial effects to consumers, usually by restoring or improving gut microflora. Goat milk is one of the rich sources of probiotics as well as nutrients. Therefore, the primary aim of this research was to isolate and evaluate the potential of novel indigenous probiotic strains present in goat milk. Six different raw goat milk samples were collected from different areas of Multan, Pakistan. For bacterial characterization, samples were cultured and isolated on MRS agar plates for different morphological and biochemical tests. The probiotic potential of the six isolates, all of which were gram positive (G1, G2, G3, G4, G5, and G6) and five of which were catalase negative (all except G1), were assessed via a milk coagulation assay and antimicrobial activity, pH tolerance, phenol tolerance, and sodium chloride (NaCl) tolerance tests, which revealed that all the isolates coagulated in milk and showed protease and lipase activity, except G3. All six isolates showed tolerance against 0.2% phenol and 2–4% NaCl and were able to survive in both alkaline and acidic conditions. Only five isolates showed antimicrobial activity against indicator strain Aspergillus niger strain STA9, validating their probiotic nature. The most potent bile-tolerant and bacteriocin-producing isolate, G1, also showed γ-hemolytic activity and resistance to penicillin but showed susceptibility to other antibiotics. The lactic acid-producing (0.60% titratable acidity) G1 isolate was identified as a novel strain of Mammaliicoccus sciuri based on 16S rDNA sequencing. The above findings suggest that the potent M. sciuri GMN01 strain can serve as a potential probiotic strain. A potent probiotic strain isolated from raw goat milk could be utilized as a dietary supplement, and goat milk could become an alternative to other sources of milk, particularly cow milk. However, safety aspects of this strain require further investigation because the present safety tests are insufficient to conclude that the GMN01 isolate is safe.

show abstract

“…Ее принимают при колите, для снижения риска развития диареи во время приема антибиотиков, для улучшения состояния при СРК (синдроме раздраженного кишечника). [3]…”

unclassified

Простокваша: Состав И Преимущества, Доказанные Исследованиями

Елисеева Татьяна

2022

ЖЗПД

View full text Add to dashboard Cite

В статье рассмотрены основные свойства простокваши и её воздействие на организм человека. Проведен систематический обзор современной специализированной литературы и актуальных научных данных. Указан химический состав и пищевая ценность продукта, рассмотрено использование простокваши в различных видах медицины и эффективность её применения при различных заболеваниях. Отдельно проанализированы потенциально неблагоприятные эффекты простокваши на организм человека при определенных медицинских состояниях и заболеваниях. Расширенная HTML версия статьи приведена на сайте edaplus.info.

show abstract

Isolation of Lactobacillus strain from curdled milk and investigation of their antimycotoxinogen activity

Cited by 4 publications

References 28 publications

A scalable overexpression of a thermostable recombinant poly-histidine tag carboxyl esterase under lambda promoter: purification, characterization, and protein modelling

A scalable overexpression of a thermostable recombinant poly-histidine tag carboxyl esterase under lambda promoter: purification, characterization, and protein modelling

First report on the probiotic potential of <i>Mammaliicoccus sciuri</i> isolated from raw goat milk

Простокваша: Состав И Преимущества, Доказанные Исследованиями

Contact Info

Product

Resources

About