Isolation of<i>frankia</i>strains from root nodules of<i>myrica rubra</i>

Hiyoshi, Tora; Sasakawa, Hideo; Yatazawa, Michihiko

doi:10.1080/00380768.1988.10415584

Cited by 10 publications

(6 citation statements)

References 18 publications

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“…Frankia strains AH1, AHm01, CaE04, EP01, EU05, and EUr01 were isolated using the differential filtration method [27] from root nodules collected in the field (Table 1). Lobes of fresh nodules were sterilized with 1% sodium hypochlorite for 5 min, washed with sterilized water, and homogenized in a mortar.…”

Section: Methodsmentioning

confidence: 99%

Different dynamics of genome content shuffling among host-specificity groups of the symbiotic actinobacterium Frankia

et al. 2014

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BackgroundFrankia is a genus of soil actinobacteria forming nitrogen-fixing root-nodule symbiotic relationships with non-leguminous woody plant species, collectively called actinorhizals, from eight dicotyledonous families. Frankia strains are classified into four host-specificity groups (HSGs), each of which exhibits a distinct host range. Genome sizes of representative strains of Alnus, Casuarina, and Elaeagnus HSGs are highly diverged and are positively correlated with the size of their host ranges.ResultsThe content and size of 12 Frankia genomes were investigated by in silico comparative genome hybridization and pulsed-field gel electrophoresis, respectively. Data were collected from four query strains of each HSG and compared with those of reference strains possessing completely sequenced genomes. The degree of difference in genome content between query and reference strains varied depending on HSG. Elaeagnus query strains were missing the greatest number (22–32%) of genes compared with the corresponding reference genome; Casuarina query strains lacked the fewest (0–4%), with Alnus query strains intermediate (14–18%). In spite of the remarkable gene loss, genome sizes of Alnus and Elaeagnus query strains were larger than would be expected based on total length of the absent genes. In contrast, Casuarina query strains had smaller genomes than expected.ConclusionsThe positive correlation between genome size and host range held true across all investigated strains, supporting the hypothesis that size and genome content differences are responsible for observed diversity in host plants and host plant biogeography among Frankia strains. In addition, our results suggest that different dynamics of shuffling of genome content have contributed to these symbiotic and biogeographic adaptations. Elaeagnus strains, and to a lesser extent Alnus strains, have gained and lost many genes to adapt to a wide range of environments and host plants. Conversely, rather than acquiring new genes, Casuarina strains have discarded genes to reduce genome size, suggesting an evolutionary orientation towards existence as specialist symbionts.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-609) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Different dynamics of genome content shuffling among host-specificity groups of the symbiotic actinobacterium Frankia

et al. 2014

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“…Most actinorhizal plants are capable of achieving high rates of nitrogen fixation (Dillon and Baker 1982; Fukumoto et al . 1992; Hiyoshi et al . 1988; Sasakawa 1995; Tani et al .…”

Section: Introductionmentioning

confidence: 99%

ORIGINAL ARTICLE: Host range ofFrankiastrains isolated from actinorhizal plants growing in Japan and their relatedness based on 16S rDNA

Nagashima

Tani

Yamamoto

et al. 2008

Soil Science and Plant Nutrition

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CorrigendumThe publisher would like to draw the reader's attention to an error in the following article: Nagashima Y, Tani C, Yamamoto M and Sasakawa H: Host range of Frankia strains isolated from actinorhizal plants growing in Japan and their relatedness based on 16S rDNA. Soil Sci. and Plant. Nutr., 54, 379-386.On page 383, table 3 was published incorrectly.The article is reprinted on the following pages in it's entirety with the corrected table.The publisher apologizes for this error and any confusion it may have caused. Readers are advised to cite the original article (citation details above) and not this Corrigendum. AbstractThe host range of seven Frankia strains isolated from the root nodules of five actinorhizal plants in four genera growing in Japan and their relatedness based on 16S rDNA were examined. The Ceq1 strain isolated from Casuarina equisetifolia formed many nodules with high nitrogen fixation activity on the source host, C. equisetifolia. The Ceq1 strain formed nodules on Alnus sieboldiana, Alnus hirsta and Myrica rubra, but their nodulation ratio and nitrogen fixation activity were very low. The Asi1 strain isolated from A. sieboldiana, the Ahi1 strain isolated from A. hirsta, and the Mru1, Mru2 and Mru8 strains isolated from M. rubra formed many nodules on both Alnus species and M. rubra. The Ema2 strain isolated from Elaeagnus macrophylla formed nodules on both Elaeagnus species and M. rubra. Based on the aspect of nodulation and nitrogen fixation activity, Ceq1 strain is an effective strain only for Casuarina, five strains of Asi1, Ahi1, Mru1, Mru2 and Mru8 are effective strains for both Alnus and Myrica, and Ema2 strain is an effective strain for both Elaeagnus and Myrica. The results of phylogenetic analyses based on 16S rDNA sequences showed that seven strains were separated into two major clusters. Cluster 1 was further grouped into two subclusters, 1a and 1b. The Asi1, Ahi1, Mru1, Mru2 and Mru8 strains belonged to 1a and the Ceq1 strain belonged to cluster 1b. The Ema2 strain was classified into cluster 2. This phylogenetic classification of Frankia based on 16S rDNA sequences appears to be consistent with the host range determined using an inoculation test.

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“…We isolated an effective Frankia strain from the nodules of Myrica rubra (Hiyoshi et al 1988). Here we designate the strain as MrIl.…”

Section: Methodsmentioning

confidence: 99%

“…In Japan, species distributed among Alnus, Myrica, Elaeagnus, Casuarina, and Coriaria grow naturally. Most of the actinorhizal plants are capable of achieving higher rates of nitrogen fixation (Dilton and Baker 1982;Hiyoshi et al 1988;Silvester and Harris 1989;Fukumoto et al 1992) and the estimated amount of nitrogen fixed by those plants annually per unit area is comparable to that by legumes (Hibbs and Cromack 1990;Kondo and Yoneyama 1990).…”

mentioning

confidence: 99%

Isolation offrankiastrains from root nodules ofmyrica rubra

Cited by 10 publications

References 18 publications

Different dynamics of genome content shuffling among host-specificity groups of the symbiotic actinobacterium Frankia

Different dynamics of genome content shuffling among host-specificity groups of the symbiotic actinobacterium Frankia

ORIGINAL ARTICLE: Host range ofFrankiastrains isolated from actinorhizal plants growing in Japan and their relatedness based on 16S rDNA

Effect ofFrankiaInoculation on Growth and Nitrogen-Fixing Activity ofMyrica ruhraSeedlings Prepared Aseptically

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