Isolation of Campylobacter pyloridis from Human Gastric Mucosa and Characterization of the Isolates

Abstract

Biopsy specimens of human gastric mucosa of patients with gastric complaints and subjected to endoscopic examination were cultured microaerobically, and Campylobacter pyloridis was detected in 46 out of 80 cases (57.5%). The organism was found in 13 out of 22 patients with gastritis, 11 out of 16 with gastric ulcer scar, 7 out of 16 with gastric ulcer, 3 out of 9 with gastric polyp, 4 out of 5 with gastric carcinoma, 2 out of 2 with esophagus carcinoma, and 6 out of 9 with other gastric diseases. The isolates were identified as C. pyloridis, demonstrating its characteristic features such as positive for oxidase and catalase, negative for reduction of nitrite and nitrate, positive for urease, no growth at 25 C, growth at 37 C, not tolerant to 1% glycine, and resistant to nalidixic acid. Positive alkaline phosphatase activity was considered as an additional feature characteristic for the strains of C. pyloridis. The major cellular fatty acids were tetradecanoic acid and 19-carbon-cyclopropane acid. This pattern is unique among Campylobacter species. The survival of the organism for a longer period than 60 min at pH 2.5 indicates its significant resistance to acidic environment.

Bacterial activity of a new antiulcer agent, ecabet sodium, against Helicobacter pylori under acidic conditions

“…The optimal pH range for growth of H. pylori has been reported to be 6.0 to 8.0, and the organism cannot survive at pHs below 3.0 (16,20). However, H. pylori has been frequently isolated from the gastric juice of patients at pHs below 3.0 (16).…”

Minimal standards for describing new species belonging to the families Campylobacteraceae and Helicobacteraceae: Campylobacter, Arcobacter, Helicobacter and Wolinella spp.

“…Results for the following tests are required: (i) oxidase activity, by use of any conventional method [28]; (ii) catalase activity, with percentage of reagent solution and time of observation given; (iii) nitrate reduction, preferably by the plate method of Cook [38]; (iv) indoxyl acetate hydrolysis, preferably using a disc method [28] with percentage of reagent solution, volume of impregnation and time of observation given; (v) urease activity, using a rapid method [39]; (vi) alkaline phosphatase activity [40], with time of observation given; (vii) hippurate hydrolysis [41]; and (viii) selenite reduction [28]. Growth on media containing the following compounds using standardized methods [26,27] should be determined: 2.0 % NaCl, 1 % glycine and 0.04 % triphenyl tetrazolium chloride (TTC): the ability of strains to reduce the latter should also be recorded when growth is observed.…”

Antibacterial Activity of Hydrolyzable Tannins Derived from Medicinal Plants against Helicobacter pylori

“…In this study, two standard strains (NCTC 11638 and ATCC 43504) and 30 clinical isolates of H. pylori were used. The clinical isolates were obtained from nine patients with chronic gastritis, ten patients with gastric ulcer and eleven patients with duodenal ulcer, and their identification was based on standard biochemical tests (22). Stock cultures were stored at Ϫ80 C in brain heart infusion broth (Difco, Detroit, Mich., U.S.A.) supplemented with 10% horse serum and 10% glycerol.…”