Isolation of C. dubliniensis from insulin‐using diabetes mellitus patients

Abstract

The identification of the novel candidal species, C. dubliniensis, from oral swab studies of HIV-seropositive and -seronegative individuals has led to speculation that such a species may also reside in the oral cavity of other patient groups. In this study involvement of the newly described species, C. dubliniensis, was investigated in oral carriage and disease in 414 insulin-using diabetes mellitus patients. Seventy-seven percent of the diabetic patients carried candidal species in the oral cavity. C. albicans was the most commonly identified candidal species. This was followed by C. dubliniensis, which was isolated on 64 occasions. Colonisation with multiple candidal species was common, and C. dubliniensis was present in both carriage and disease states. Seven patients without signs of oral disease had C. dubliniensis isolated as the sole candidal species, while the same species was associated with various forms of oral candidosis in six patients.

Use of denaturing gradient gel electrophoresis for the identification of mixed oral yeasts in human saliva

“…Our isolation of C. dubliniensis together with C. albicans is in agreement with previous reports (Willis et al, 2000). This apparently high prevalence of C. dubliniensis may reflect misidentification in some earlier studies, as in the present study C. dubliniensis could not be identified reliably by CHROMagar Candida.…”

Incidence and Virulence Traits of Candida Dubliniensis Isolated From Clinically Suspected Patients

“…Due to the use of inefficient sampling and identification methods or the sampling on the wrong anatomic sites, the prevalence of C. dubliniensis in normal healthy individuals has been underestimated probably [2]. In a study reported that, on an Irish population of normal healthy individuals, only 3.5% of individuals were found to carry C. dubliniensis in the oral cavity while the prevalence of this species in the vagina was found to be even lower [24] and relatively high prevalence rates of C. dubliniensis have been found in oral cavities of patients suffering from diabetes [22]. As well as being associated with oral infections, C. dubliniensis has also been identified in a wide range of other anatomic sites [2].…”

“…Despite the effort that has been expended to identify C. dubliniensis in clinical samples, a definitive assessment of the prevalence of this species is still lacking [2]. C. dubliniensis have been isolated from a wide range of geographical locations including Europe, North and South America, and Australia [22]. It recovered from several body sites in many populations, but it is most frequently recovered from the oral cavities of HIV-infected patients [6], and 15-30% has been reported from oral cavities of HIV-infected and AIDS patients [23].…”

Distribution of Candida dubliniensis from Denture Membrane Surfaces

“…Diabetic and HIV-negative individuals also exhibited a high prevalence of C. dubliniensis in oral carriage and disease states (2). As C. dubliniensis is very closely related to Candida albicans, it is difficult to distinguish these species from individual clinical samples.…”